LOCUS       sequence-343665-        4652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp13
FEATURES             Location/Qualifiers
     source          1..4652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1963..2062)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2079..2098)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2080..2098)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2103..2119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2103..2119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2550..3341
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3434..4022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3923..3942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4176..4193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4352..4379)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4471..4557)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4606..4628
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4620..4637
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4621..4637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg ctgtaggtgc ttgtgtattg tgcaattcac agacttcact tcgttgcggt
      181 gcctgtatta ggaggccatt cctatgttgc aagtgctgct atgaccatgt catttcaaca
      241 tcacacaaat tagtgttgtc tgttaatccc tatgtttgca atgccccagg ttgtgatgtc
      301 actgatgtga cacaactgta tctaggaggt atgagctatt attgcaagtc acataagcct
      361 cccattagtt ttccattatg tgctaatggt caggtttttg gtttatacaa aaacacatgt
      421 gtaggcagtg acaatgtcac tgacttcaat gcgatagcaa catgtgattg gactaatgct
      481 ggcgattaca tacttgccaa cacttgtact gagagactca agcttttcgc agcagaaacg
      541 ctcaaagcca ctgaggaaac atttaagctg tcatatggta ttgccactgt acgcgaagta
      601 ctctctgaca gagaattgca tctttcatgg gaggttggaa aacctagacc accattgaac
      661 agaaactatg tctttactgg ttaccgtgta actaaaaata gtaaagtaca gattggagag
      721 tacacctttg aaaaaggtga ctatggtgat gctgttgtgt acagaggtac tacgacatac
      781 aagttgaatg ttggtgatta ctttgtgttg acatctcaca ctgtaatgcc acttagtgca
      841 cctactctag tgccacaaga gcactatgtg agaattactg gcttgtaccc aacactcaac
      901 atctcagatg agttttctag caatgttgca aattatcaaa aggtcggcat gcaaaagtac
      961 tctacactcc aaggaccacc tggtactggt aagagtcatt ttgccatcgg acttgctctc
     1021 tattacccat ctgctcgcat agtgtatacg gcatgctctc atgcagctgt tgatgcccta
     1081 tgtgaaaagg cattaaaata tttgcccata gataaatgta gtagaatcat acctgcgcgt
     1141 gcgcgcgtag agtgttttga taaattcaaa gtgaattcaa cactagaaca gtatgttttc
     1201 tgcactgtaa atgcattgcc agaaacaact gctgacattg tagtctttga tgaaatctct
     1261 atggctacta attatgactt gagtgttgtc aatgctagac ttcgtgcaaa acactacgtc
     1321 tatattggcg atcctgctca attaccagcc ccccgcacat tgctgactaa aggcacacta
     1381 gaaccagaat attttaattc agtgtgcaga cttatgaaaa caataggtcc agacatgttc
     1441 cttggaactt gtcgccgttg tcctgctgaa attgttgaca ctgtgagtgc tttagtttat
     1501 gacaataagc taaaagcaca caaggataag tcagctcaat gcttcaaaat gttctacaaa
     1561 ggtgttatta cacatgatgt ttcatctgca atcaacagac ctcaaatagg cgttgtaaga
     1621 gaatttctta cacgcaatcc tgcttggaga aaagctgttt ttatctcacc ttataattca
     1681 cagaacgctg tagcttcaaa aatcttagga ttgcctacgc agactgttga ttcatcacag
     1741 ggttctgaat atgactatgt catattcaca caaactactg aaacagcaca ctcttgtaat
     1801 gtcaaccgct tcaatgtggc tatcacaagg gcaaaaattg gcattttgtg cataatgtct
     1861 gatagagatc tttatgacaa actgcaattt acaagtctag aaataccacg tcgcaatgtg
     1921 gctacattac aacttaatta agacgcgatc gcggcctcat gggcccagct ttcttgtaca
     1981 aagttggcat tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca
     2041 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2101 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2161 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     2221 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     2281 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2341 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2401 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2461 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2521 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     2581 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2641 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2701 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2761 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2821 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2881 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2941 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3001 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     3061 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     3121 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     3181 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3241 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3301 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     3361 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4081 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4141 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4201 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4261 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     4321 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4381 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4441 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4501 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4561 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     4621 gtaaaacgac ggccagtctt aagctcgggc cc
//
