LOCUS       sequence-343686-        4763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp2
FEATURES             Location/Qualifiers
     source          1..4763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2074..2173)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2190..2209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2191..2209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2214..2230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2214..2230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2661..3452
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3545..4133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4034..4053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4287..4304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4463..4490)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4582..4668)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4717..4739
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4731..4748
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4732..4748
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg cagtcactcg ctatgtcgac aacaatttct gtggcccaga tgggtaccct
      181 cttgattgca tcaaagattt tctcgcacgc gcgggcaagt caatgtgcac tctttccgaa
      241 caacttgatt acatcgagtc gaagagaggt gtctactgct gccgtgacca tgagcatgaa
      301 attgcctggt tcactgagcg ctctgataag agctacgagc accagacacc cttcgaaatt
      361 aagagtgcca agaaatttga cactttcaaa ggggaatgcc caaagtttgt gtttcctctt
      421 aactcaaaag tcaaagtcat tcaaccacgt gttgaaaaga aaaagactga gggtttcatg
      481 gggcgtatac gctctgtgta ccctgttgca tctccacagg agtgtaacaa tatgcacttg
      541 tctaccttga tgaaatgtaa tcattgcgat gaagtttcat ggcagacgtg cgactttctg
      601 aaagccactt gtgaacattg tggcactgaa aatttagtta ttgaaggacc tactacatgt
      661 gggtacctac ctactaatgc tgtagtgaaa atgccatgtc ctgcctgtca agacccagag
      721 attggacctg agcatagtgt tgcagattat cacaaccact caaacattga aactcgactc
      781 cgcaagggag gtaggactag atgttttgga ggctgtgtgt ttgcctatgt tggctgctat
      841 aataagcgtg cctactgggt tcctcgtgct agtgctgata ttggctcagg ccatactggc
      901 attactggtg acaatgtgga aaccttgaat gaggatctcc ttgagatact gagtcgtgaa
      961 cgtgttaaca ttaacattgt tggcgatttt catttgaatg aagaggttgc catcattttg
     1021 gcatctttct ctgcttctac aagtgccttt attgacacta taaagagtct tgattacaag
     1081 tctttcaaaa ccattgttga gtcctgcggt aactataaag ttaccaaggg aaagcccgta
     1141 aaaggtgctt ggaacattgg acaacagaga tcagttttaa caccactgtg tggttttccc
     1201 tcacaggctg ctggtgttat cagatcaatt tttgcgcgca cacttgatgc agcaaaccac
     1261 tcaattcctg atttgcaaag agcagctgtc accatacttg atggtatttc tgaacagtca
     1321 ttacgtcttg tcgacgccat ggtttatact tcagacctgc tcaccaacag tgtcattatt
     1381 atggcatatg taactggtgg tcttgtacaa cagacttctc agtggttgtc taatcttttg
     1441 ggcactactg ttgaaaaact caggcctatc tttgaatgga ttgaggcgaa acttagtgca
     1501 ggagttgaat ttctcaagga tgcttgggag attctcaaat ttctcattac aggtgttttt
     1561 gacatcgtca agggtcaaat acaggttgct tcagataaca tcaaggattg tgtaaaatgc
     1621 ttcattgatg ttgttaacaa ggcactcgaa atgtgcattg atcaagtcac tatcgctggc
     1681 gcaaagttgc gatcactcaa cttaggtgaa gtcttcatcg ctcaaagcaa gggactttac
     1741 cgtcagtgta tacgtggcaa ggagcagctg caactactca tgcctcttaa ggcaccaaaa
     1801 gaagtaacct ttcttgaagg tgattcacat gacacagtac ttacctctga ggaggttgtt
     1861 ctcaagaacg gtgaactcga agcactcgag acgcccgttg atagcttcac aaatggagct
     1921 atcgttggca caccagtctg tgtaaatggc ctcatgctct tagagattaa ggacaaagaa
     1981 caatactgcg cattgtctcc tggtttactg gctacaaaca atgtctttcg cttaaaaggg
     2041 ggtcttaatt aagacgcgat cgcggcctca tgggcccagc tttcttgtac aaagttggca
     2101 ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2161 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2221 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2281 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2341 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2401 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2461 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2521 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2581 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2641 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2701 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2761 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2821 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2881 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2941 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3001 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3061 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3121 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3181 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3241 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3301 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3361 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3421 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3481 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4201 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4261 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4381 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4441 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4501 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4561 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4621 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     4681 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     4741 cggccagtct taagctcggg ccc
//
