LOCUS       sequence-343688-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of murine Flag-Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt1
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2812..2877
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4332..4904
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4355..4906
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4499..4516)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4906..6336
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    6350..6938
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6403..6423)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6821..6832)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7145..7778
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7906..7922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7906..7922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7919..7941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7930..7946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7954..7960,7961..7978,7979..7984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7999..8020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8137..8154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8308..8896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8388..8407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9067..9858,9859..9927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9690..9709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9928..10032)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10080..10214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10117..10136)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10171..10190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcat ggactacaaa
     2821 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt
     2881 atgaacgacg tagccattgt gaaggagggc tggctgcaca aacgagggga atatattaaa
     2941 acctggcggc cacgctactt cctcctcaag aacgatggca cctttattgg ctacaaggaa
     3001 cggcctcagg atgtggatca gcgagagtcc ccactcaaca acttctcagt ggcacaatgc
     3061 cagctgatga agacagagcg gccaaggccc aacaccttta tcatccgctg cctgcagtgg
     3121 accacagtca ttgagcgcac cttccatgtg gaaacgcctg aggagcggga agaatgggcc
     3181 accgccattc agactgtggc agatggactc aagaggcagg aagaagagac gatggacttc
     3241 cgatcaggct cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag
     3301 cccaagcacc gtgtgaccat gaacgagttt gagtacctga agctactggg caagggcacc
     3361 tttgggaagg tgattctggt gaaagagaag gccacaggcc gctactatgc catgaagatc
     3421 ctcaagaagg aggtcatcgt cgccaaggat gaggttgccc acacgcttac tgagaaccgc
     3481 gtgcttcaga actctaggca tcccttcctt acggccctca agtactcatt ccagacccac
     3541 gaccgcctct gctttgtcat ggagtatgcc aacgggggcg agctcttctt ccacctgtct
     3601 cgagagcgtg tgttctccga ggaccgggcc cgcttctatg gtgcggagat tgtgtctgcc
     3661 ctggactact tgcactccga gaagaacgtg gtgtaccggg acctgaagct ggagaacctc
     3721 atgctggaca aggatgggca catcaagata acggacttcg ggctgtgcaa ggaggggatc
     3781 aaggacggtg ccactatgaa gacattctgc ggaacgccgg agtacctggc ccctgaggtg
     3841 ctggaggaca acgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac
     3901 gagatgatgt gtggccgcct gcccttctac aaccaggacc acgagaagct gttcgagctg
     3961 atcctcatgg aggagatccg cttcccgcgc acactcggcc ctgaggccaa gtccctgctc
     4021 tccgggctgc tcaagaagga ccctacacag aggctcggtg ggggctccga ggatgccaag
     4081 gagatcatgc agcaccggtt ctttgccaac atcgtgtggc aggatgtgta tgagaagaag
     4141 ctgagcccac ctttcaagcc ccaggtcacc tctgagactg acaccaggta tttcgatgag
     4201 gagttcacag ctcagatgat caccatcacg ccgcctgatc aagatgacag catggagtgt
     4261 gtggacagtg agcggaggcc gcacttcccc cagttctcct actcagccag tggcacagcc
     4321 tgaggatccc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     4381 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     4441 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     4501 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     4561 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     4621 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     4681 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc
     4741 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     4801 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc
     4861 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggt gagcaagggc
     4921 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     4981 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5041 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     5101 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     5161 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     5221 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     5281 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5341 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     5401 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     5461 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     5521 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     5581 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     5641 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     5701 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5761 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     5821 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     5881 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5941 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     6001 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     6061 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     6121 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     6181 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     6241 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     6301 ctgttcctgt acggcatgga cgagctgtac aagtgaacgc gtctggaaca atcaacctct
     6361 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6421 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6481 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6541 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6601 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6661 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6721 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6781 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6841 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6901 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     6961 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7021 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7081 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     7141 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7201 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7261 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7321 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7381 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7441 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7501 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7561 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7801 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     7861 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10021 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10201 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10261 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10321 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10381 t
//
