LOCUS       sequence-343692-       16397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16397)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-nl.EGFP-tdTomato
FEATURES             Location/Qualifiers
     source          1..16397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3370..3391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3631..3650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3978..3999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4054..4074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4078..4098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4102..4122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4221..5651
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             7826..7837
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9629..9640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12836..12854)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12850..12897
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12925..12941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12925..12941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12938..12960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12949..12965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12973..12979,12980..12997,12998..13003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13018..13039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13156..13173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13327..13915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13407..13426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14086..14877,14878..14946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14709..14728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14947..15051)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15078..15534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15165..15184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15375..15396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15660..15682
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15674..15691
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15675..15691
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15761..16064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16065..16268
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16219..16239
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16313..16332
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16313..16331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa ggctcgagct gatccaaaaa agaagagaaa ggtagatcca
     4081 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccag ataactgatc
     4141 ataatcagcc ataccacatt tggaattcgt cgagggggcg cgccgctaga catgaaaaaa
     4201 actaacactc ctccggtacc atggtgagca agggcgagga ggtcatcaaa gagttcatgc
     4261 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     4321 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     4381 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     4441 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     4501 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     4561 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     4621 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     4681 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     4741 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     4801 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     4861 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg
     4921 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt
     4981 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     5041 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     5101 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     5161 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     5221 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     5281 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     5341 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     5401 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     5461 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     5521 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     5581 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
     5641 tgtacaagta ggctagcggc gcctagccgg tcatcctttt gacgattcca gtcccgagga
     5701 taacctcctc tcgggattgg ggggaatctt tggatccagt agtcctcctt gaactccgtc
     5761 caacagggca gattcaagag tcataagact ttcattaatc atttcagttg atcagacatg
     5821 gtcgtgtaga ttctcttaat acgggagatc ttctagcagt ttcagtgacc aacggtgctt
     5881 tcattctcca ggaactgata ccaaaggttg tggacaggcc aaggggtact tcggatgact
     5941 ctgtgcttgg gcacagaaag aggtcgtagt gtgccccccg atagcggact cgacatgaat
     6001 caactaagaa aggcaatctg cctcccatga tggacataag caatagttca caaccatctt
     6061 gcatctcagt gaagtgtaca taactattga gggctgggtc atctaagcat ttcagtcgag
     6121 aaaaaaactg tagaccaaaa gaacaactag caacacttct catccagaga cccatatcaa
     6181 gatgctagat ccgggagagg tttatgatga ccctattgat ccaattgagt cagaggctga
     6241 acccagagga acccccactg tccccaacat cttgaggaac tccgactaca atctcaattc
     6301 tcctttgata gaggatcctg ccaaactaat gttagaatgg ttgaagacag ggaacagacc
     6361 ttatcggatg actttgacag acaattgctc caggtcttac aaagttttga aagattattt
     6421 caagaaagta gatttgggtt ctctcaaagt gggcggaact gctgcacagt caatggtttc
     6481 tctctggttg tgtggtgccc actctgaatc aaacaggagc cggagatgta taaccgactt
     6541 ggcccatttc tattccaagt catcccccat agagaagcta ttgaattgta cgctaggaaa
     6601 cagaggcctg agaatcccac cagagggggt gttaaattgc ctcgagaggg tcaattatga
     6661 caaggcattt gggaggtatc tggccaacac gtattcctct tacttgtttt tccatgtaat
     6721 caccttatac atgaatgcct tagactggga agaggaaaag accatcctgg cattatggaa
     6781 agatataacc tcagtggata ccgagaagga cttggtcaaa ttcaaagatc aaatatgggg
     6841 actgttgatt gtgacaaagg actttgttta ctctcagagt tcaaactgtc tttttgacag
     6901 aaactacaca ctgatgctaa aggatctttt cttgtctcga ttcaactcct taatgattct
     6961 gctttctccc cctgagcccc gatactcaga tgacttaata tctcagctgt gccagctata
     7021 catcgctggg gatcaagtct tgtccatgtg tgggaactcc ggctatgaag tcatcaaaat
     7081 attggagcca tatgtcgtga acagtttggt ccagagggca gagaagttta ggcctctcat
     7141 ccaccccttg ggagactttc ctatgttcat aaaagacaag gtgaatcaac ttgaagggac
     7201 ttttggtccc agtgcaaaga ggttttttag ggttttagat caattcgaca acatacatga
     7261 cttagtattt gtgtatggct gttacagaca ttgggggcac ccctatatag attatcggaa
     7321 gggtctgtcg aaactatatg atcaagttca cattaagaaa gtaatagata agtcctacca
     7381 ggagtgttta gcaagtgact tggccagaag gatcctcaga tggggatttg acaagtactc
     7441 caaatggtat ctagattcga gattccttgc ccgagaccac cccttgactc cttatgtcaa
     7501 gacccaaaca tggccaccca aacatatagt agacttggtg ggggacacat ggcataagct
     7561 cccgatcacg cagatctttg aaattcctga atcaatggac ccgtcagaga tactggatga
     7621 caaatcacat tctttcacca gaacaaggtt agcttcttgg ctgtcagaga accgaggggg
     7681 gcctgttcct agcgagaagg tcattatcac ggccctgtct aagccacctg tcaatccccg
     7741 agagtttttg aaatctatag acctcggagg attgccagat gaggatttga taattggcct
     7801 caaaccaaag gaacgggagt tgaagatcga gggccgattc tttgctctaa tgtcatggaa
     7861 tctaagatta tattttgtca tcaccgaaaa gctcttggcc aactacattt tgccactttt
     7921 tgacgcactg actatgacag acaacctgaa caaggtgttc aaaaagttga tcgacagggt
     7981 caccgggcaa gggcttttgg actattctag ggtcacatac gcatttcacc tggactatga
     8041 gaaatggaac aatcatcaaa gattggagtc aacagaggat gtattctctg tcctagatca
     8101 ggtgtttgga ttgaagaggg tgttttctag aacacacgag ttttttcaga agtcctggat
     8161 ctattattca gacagatcag acctcattgg gttatgggag gaccaaatat attgcttgga
     8221 tatgtctaac ggcccaacct gctggaatgg ccaagatggc gggctagagg gcttgcggca
     8281 gaagggctgg agtctagtca gtttattaat gatagataga gaatctcaaa ccaggaacac
     8341 aagaaccaag atactagctc aaggagacaa ccaggttctg tgtcctacat acatgttgtc
     8401 accgggattg tctcaagagg ggcttctcta tgagttagag agcatatcaa ggaatgcact
     8461 ctcaatatac cgagctatcg aggaaggagc atctaagctg gggctgatca tcaagaagga
     8521 agagaccatg tgtagttatg actttctcat atatgggaag acccccttat ttcgaggcaa
     8581 catattggtg cctgaatcca aaagatgggc ccgagtctct tgcatctcta acgaccaaat
     8641 agtcaacctc gccaatataa tgtcgacagt atccaccaat gcgctgacag tggcacaaca
     8701 ctctcaatct ctgatcaaac ctatgaggga ttttctgctc atgtcagtac aggcagtttt
     8761 ccactacctg ttgtttagcc caatcctaaa aggcagagtt tataagattc tgagtgctga
     8821 aggggagagc tttctcctag ccatgtcgcg gataatctac ctagatcctt ctttgggagg
     8881 ggtgtctgga atgtctctcg ggaggttcca tatacgtcag ttctcagacc ctgtctctga
     8941 agggttgtca ttctggagag agatctggtt aggctctcat gagtcctgga ttcacgcgtt
     9001 gtgtcaggag gccgggaacc ccgatcttgg agagagaaca ctcgagagct tcactcgcct
     9061 tttagaagat cctactacct taaatatcaa aggaggggcc agccctacca ttctactcaa
     9121 ggatgctatc agaaaggctc tgtacgacga ggtggacaag gtggaaaatt cagagtttcg
     9181 agaggcaatc ctgctgtcca agacccatag agataacttt atactctttt taaaatctgt
     9241 tgagcctctg ttccctcgat ttctcagtga gctcttcagt tcgtctttct tgggaatacc
     9301 ggagtcaatc attggactga tacaaaactc ccggacaata agaaggcagt ttagaaagag
     9361 tctctcaaga actttagaag agtccttcta caactcagag atccacggga ttaatcggat
     9421 aacccagaca cctcaaaggg tcggaagggt gtggccttgc tcttcagaga gggcagatct
     9481 acttagggag atctcttggg ggaggaaagt ggtaggcacg acagttcctc acccttccga
     9541 gatgttggag ttgtttccca aatcctccat ttcctgcact tgtggagcaa cagggggagg
     9601 caatcctaga gtctctgtat cagtactccc gtccttcgat cagtcatttt tctcacgggg
     9661 ccccctaaag ggatacttgg gctcgtccac ctccatgtca acccagctat tccatgcatg
     9721 ggaaaaagtc actaatgttc atgtggtgaa aagggctcta tcgttaaaag aatctataaa
     9781 ctggttcatc aataggaatt ccaatttggc tcaaactcta attggaaaca tcatgtctct
     9841 gacaggccct gatttccctc tagaagaggc ccctgttttc aaacggacag ggtcagcctt
     9901 gcataggttc aagtctgcca gatacagcga aggagggtat tcttctgttt gccctaacct
     9961 tctctcccat atctctgtta gtacagacac tatgtctgat ttgacccaaa acgggaagaa
    10021 ctatgatttc atgtttcagc cattgatgct ttatgcgcaa acatggacat cggaactggt
    10081 acagagggat acaagactta gagactccac gtttcactgg caccttcggt gcaacagatg
    10141 tgtgaggccc attgatgata taacactgga aacttctcag atcttcgagt tcccggatgt
    10201 gtcaaaaagg atatccagga tggtttctgg agctgtccct caatttcaga agcttcctga
    10261 tatccgtcta agaccaggtg attttgaatc cctaagtggt agagaaaagt ctcgccatat
    10321 agggtcagct caggggctct tatactcaat cttagtagca attcacgact caggatacaa
    10381 tgatgggacc atcttccctg tcaacatata cggcaaagtt tcccccagag actatttgag
    10441 agggcttgca agagggatct tgatagggtc ctcgatttgc ttcttgacac gaatgacaaa
    10501 tattaacatt aacagacctc ttgaattgat ctcaggggta atttcctata ttctcctgag
    10561 gctggataat catccctctc tgtatataat gcttagagaa ccgtctctta gaggagaaat
    10621 attctctatc cctcagaaaa tccccgccgc ttacccaacc actatgagag aaggcaacag
    10681 atcgattttg tgttacctcc aacacgtgct acgctatgag cgagaggcaa tcacggcgtc
    10741 cccggagaat gactggctgt ggatcttctc agacttcaga agtgtgaaaa tgacgtactt
    10801 gaccctcatt acctaccagt ctcacctcct actccagagg gttgagagaa acttgtctaa
    10861 gagtatgaga gctactctgc gacaaatgag ttccttaatg aggcaagtgc tgggtgggca
    10921 cggagaagat accttggagt cagacgatga cattcaacga ttactaaaag actctttgcg
    10981 aaagacaagg tgggtggatc aagaggtgcg ccatgcagct agaaccatga atggagatta
    11041 cagccccgac aagaaagtat cccacaaggc aggatgttca gaatgggtct gctctgctca
    11101 acagattgcc gtctccacct cagccaaccc ggcccctgtc tcagagcttg acattagggc
    11161 cctctctaag aggtttcaaa accccttgat ctcgggcctg agagtggttc agtgggcaac
    11221 cggtgcccat tataagctta agcctattct agatgatcta aatgttttcc catctctctg
    11281 tcttgtaatt ggagacgggt cagggggaat atcaagggca gttctcaaca tgtttccaga
    11341 ttctaagctt gtgttcaaca gcctattgga ggtgaatgat ctgatggctt ccggaacaca
    11401 tccactgcct ccttcagcaa tcatgagtgg aggagatgac atcatctcca gagtgataga
    11461 ctttgactca atatgggaga aaccgtccga cctgaggaac tcggccacct ggagatactt
    11521 ccagtcggtt caaaaacagg tcaacatgtc gtatgacctc attatttgtg atgcagaagt
    11581 tactgatatt gcatctatca accggataac tctgttgatg tctgatttcg cattgtctat
    11641 agatggacca ctttatctgg tcttcaaaac ttacgggact atgctagtaa acccggacta
    11701 taaagctatt caacatctgt caagagcgtt cccttcggtc acagggtttg taacccaagt
    11761 aacttcatcc ttttcttctg agctatacct ccggttctct aaacgaggaa agtttttcag
    11821 ggacgccgag tacttgacct cttccaccct tcgagagatg agccttgtgt tgttcaattg
    11881 cagcagcccc aaaagtgaga tgcagagagc tcgttcctta aactatcaag acctggtaag
    11941 gggatttcct gaagagatca tatcaaatcc ttacaacgaa atgatcataa ctctgattga
    12001 caatgatgta gagtccttcc tagtccacaa gatggtggat gatcttgagc tacagagggg
    12061 aactctgtct aaagtggcta tcattatatc catcatgatc gttttttcca atagagtctt
    12121 caacatttcc aaacctttga ctgacccctt gttctacccc ccatctgatc ctaaaatcct
    12181 gaggcacttc aacatatgtt gcagtactat gatgtatcta tctaccgctt taggcgacgt
    12241 ccctaacttc gcaagacttc atgacctgta taacagacct ataacttgtt acttcagaaa
    12301 acaagttatt cgagggaata tttatctatc ttggagttgg tccgatgata ccccagtgtt
    12361 caagagagta gcctgtaatt ctagcttgag tctgtcatct cactggatca ggttgatcta
    12421 caagatagtg aagactacca gactcattgg cagcataaaa gacctatcag gagaggtaga
    12481 acgacatctt catgggtata acagatggat caccctcgag gatatccgat ctagatcatc
    12541 cctactagat tacagttgtt tgtaagccgg atattaccga aagcctgtgc atgctaaaat
    12601 tcttgtatga tgcatcttga aaaaaacaag atcttgaatc cggacctctg gttgtttgat
    12661 tgttttttcc atctttattg tttttttgtt aagcgtgggt cggcatggca tctccacctc
    12721 ctcgcggtcc gacctgggca tccgaaggag gacgcacgtc cactcggatg gctaagggag
    12781 ggcggggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
    12841 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
    12901 aaagtcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12961 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    13021 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13081 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    14041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    14101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    14161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    14221 accgcgagac ccacccttgg aggctccaga tttatcagca ataaaccagc cagccggaag
    14281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    14341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    14401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    14461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    14521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    14581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    14641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    14701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    15001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    15061 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    15121 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    15181 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    15241 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    15301 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    15361 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    15421 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaaatgagc
    15481 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
    15541 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    15601 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    15661 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tagttattaa tagtaatcaa
    15721 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    15781 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    15841 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    15901 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    15961 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    16021 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    16081 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    16141 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    16201 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    16261 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
    16321 ctcactatag ggagacccaa gctggctaga ttaagcgtct gatgagtccg tgaggacgaa
    16381 acccggcgta ccgggtc
//
