LOCUS       sequence-343693-       15769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15769)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 15769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-SypRFP
FEATURES             Location/Qualifiers
     source          1..15769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3323..3332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4283..4292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4289..5020
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             7198..7209
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9001..9012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12208..12226)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12222..12269
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12297..12313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12297..12313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12310..12332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12321..12337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12345..12351,12352..12369,12370..12375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12390..12411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12528..12545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12699..13287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12779..12798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13458..14249,14250..14318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14081..14100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14319..14423)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14450..14906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14537..14556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14747..14768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15032..15054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15046..15063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15047..15063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15133..15436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15437..15640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15591..15611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15685..15704
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15685..15703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggtacgg ccgccaccat ggacgtggtg aatcagctgg tggctggggg
     3361 tcagttccgg gtggtcaagg agccccttgg cttcgtgaag gtgctgcagt gggtctttgc
     3421 catcttcgcc tttgctacgt gcggcagcta caccggagag cttcggctga gcgtggagtg
     3481 tgccaacaag acggagagtg ccctcaacat cgaagtcgaa tttgagtacc cattcaggct
     3541 gcaccaagtg tactttgatg caccctcctg cgttaaaggg ggcactacca agatcttcct
     3601 agttggtgac tactcctcct cgggtgaatt ctttgtcacc gtggctgtgt ttgccttcct
     3661 ctactccatg ggggccctgg ccacctacat cttcctgcag aacaagtacc gagagaacaa
     3721 caaagggcca atgatggact tcctggccac agcagtgttc gctttcatgt ggctagttag
     3781 ctcatccgcc tgggccaaag gcctgtccga tgtgaagatg gccactgacc cagagaacat
     3841 tatcaaggag atgcctatgt gccgccagac aggaaacaca tgcaaggaac tgagggaccc
     3901 tgtgacttca ggactcaaca cctcggtggt gtttggcttc ctgaacctgg tgctctgggt
     3961 tggcaaccta tggttcgtgt tcaaggagac aggctgggcc gccccattca tgcgcgcacc
     4021 tccaggcgcc ccagaaaagc aaccagctcc tggcgatgcc tacggcgatg cgggctatgg
     4081 gcagggcccc ggaggctatg ggccccagga ctcctacggg cctcagggtg gttatcaacc
     4141 cgattacggg cagccagcca gcggcggtgg cggtggctac gggcctcagg gcgactatgg
     4201 gcagcaaggc tacggccaac agggtgcgcc cacctccttc tccaatcaga tgggcggagg
     4261 ccctgggatc caccggccgg tcgccaccat ggtgtctaag ggcgaagagc tgattaagga
     4321 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
     4381 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     4441 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag
     4501 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
     4561 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     4621 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
     4681 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct
     4741 gtaccccgct gacggcggcc tggaaggcag aaccgacatg gccctgaagc tcgtgggcgg
     4801 gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct
     4861 caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa
     4921 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     4981 actggggcac aaacttaatg gcatggacga gctgtacaag taagctagcg gcgcctagcc
     5041 ggtcatcctt ttgacgattc cagtcccgag gataacctcc tctcgggatt ggggggaatc
     5101 tttggatcca gtagtcctcc ttgaactccg tccaacaggg cagattcaag agtcataaga
     5161 ctttcattaa tcatttcagt tgatcagaca tggtcgtgta gattctctta atacgggaga
     5221 tcttctagca gtttcagtga ccaacggtgc tttcattctc caggaactga taccaaaggt
     5281 tgtggacagg ccaaggggta cttcggatga ctctgtgctt gggcacagaa agaggtcgta
     5341 gtgtgccccc cgatagcgga ctcgacatga atcaactaag aaaggcaatc tgcctcccat
     5401 gatggacata agcaatagtt cacaaccatc ttgcatctca gtgaagtgta cataactatt
     5461 gagggctggg tcatctaagc atttcagtcg agaaaaaaac tgtagaccaa aagaacaact
     5521 agcaacactt ctcatccaga gacccatatc aagatgctag atccgggaga ggtttatgat
     5581 gaccctattg atccaattga gtcagaggct gaacccagag gaacccccac tgtccccaac
     5641 atcttgagga actccgacta caatctcaat tctcctttga tagaggatcc tgccaaacta
     5701 atgttagaat ggttgaagac agggaacaga ccttatcgga tgactttgac agacaattgc
     5761 tccaggtctt acaaagtttt gaaagattat ttcaagaaag tagatttggg ttctctcaaa
     5821 gtgggcggaa ctgctgcaca gtcaatggtt tctctctggt tgtgtggtgc ccactctgaa
     5881 tcaaacagga gccggagatg tataaccgac ttggcccatt tctattccaa gtcatccccc
     5941 atagagaagc tattgaattg tacgctagga aacagaggcc tgagaatccc accagagggg
     6001 gtgttaaatt gcctcgagag ggtcaattat gacaaggcat ttgggaggta tctggccaac
     6061 acgtattcct cttacttgtt tttccatgta atcaccttat acatgaatgc cttagactgg
     6121 gaagaggaaa agaccatcct ggcattatgg aaagatataa cctcagtgga taccgagaag
     6181 gacttggtca aattcaaaga tcaaatatgg ggactgttga ttgtgacaaa ggactttgtt
     6241 tactctcaga gttcaaactg tctttttgac agaaactaca cactgatgct aaaggatctt
     6301 ttcttgtctc gattcaactc cttaatgatt ctgctttctc cccctgagcc ccgatactca
     6361 gatgacttaa tatctcagct gtgccagcta tacatcgctg gggatcaagt cttgtccatg
     6421 tgtgggaact ccggctatga agtcatcaaa atattggagc catatgtcgt gaacagtttg
     6481 gtccagaggg cagagaagtt taggcctctc atccacccct tgggagactt tcctatgttc
     6541 ataaaagaca aggtgaatca acttgaaggg acttttggtc ccagtgcaaa gaggtttttt
     6601 agggttttag atcaattcga caacatacat gacttagtat ttgtgtatgg ctgttacaga
     6661 cattgggggc acccctatat agattatcgg aagggtctgt cgaaactata tgatcaagtt
     6721 cacattaaga aagtaataga taagtcctac caggagtgtt tagcaagtga cttggccaga
     6781 aggatcctca gatggggatt tgacaagtac tccaaatggt atctagattc gagattcctt
     6841 gcccgagacc accccttgac tccttatgtc aagacccaaa catggccacc caaacatata
     6901 gtagacttgg tgggggacac atggcataag ctcccgatca cgcagatctt tgaaattcct
     6961 gaatcaatgg acccgtcaga gatactggat gacaaatcac attctttcac cagaacaagg
     7021 ttagcttctt ggctgtcaga gaaccgaggg gggcctgttc ctagcgagaa ggtcattatc
     7081 acggccctgt ctaagccacc tgtcaatccc cgagagtttt tgaaatctat agacctcgga
     7141 ggattgccag atgaggattt gataattggc ctcaaaccaa aggaacggga gttgaagatc
     7201 gagggccgat tctttgctct aatgtcatgg aatctaagat tatattttgt catcaccgaa
     7261 aagctcttgg ccaactacat tttgccactt tttgacgcac tgactatgac agacaacctg
     7321 aacaaggtgt tcaaaaagtt gatcgacagg gtcaccgggc aagggctttt ggactattct
     7381 agggtcacat acgcatttca cctggactat gagaaatgga acaatcatca aagattggag
     7441 tcaacagagg atgtattctc tgtcctagat caggtgtttg gattgaagag ggtgttttct
     7501 agaacacacg agttttttca gaagtcctgg atctattatt cagacagatc agacctcatt
     7561 gggttatggg aggaccaaat atattgcttg gatatgtcta acggcccaac ctgctggaat
     7621 ggccaagatg gcgggctaga gggcttgcgg cagaagggct ggagtctagt cagtttatta
     7681 atgatagata gagaatctca aaccaggaac acaagaacca agatactagc tcaaggagac
     7741 aaccaggttc tgtgtcctac atacatgttg tcaccgggat tgtctcaaga ggggcttctc
     7801 tatgagttag agagcatatc aaggaatgca ctctcaatat accgagctat cgaggaagga
     7861 gcatctaagc tggggctgat catcaagaag gaagagacca tgtgtagtta tgactttctc
     7921 atatatggga agaccccctt atttcgaggc aacatattgg tgcctgaatc caaaagatgg
     7981 gcccgagtct cttgcatctc taacgaccaa atagtcaacc tcgccaatat aatgtcgaca
     8041 gtatccacca atgcgctgac agtggcacaa cactctcaat ctctgatcaa acctatgagg
     8101 gattttctgc tcatgtcagt acaggcagtt ttccactacc tgttgtttag cccaatccta
     8161 aaaggcagag tttataagat tctgagtgct gaaggggaga gctttctcct agccatgtcg
     8221 cggataatct acctagatcc ttctttggga ggggtgtctg gaatgtctct cgggaggttc
     8281 catatacgtc agttctcaga ccctgtctct gaagggttgt cattctggag agagatctgg
     8341 ttaggctctc atgagtcctg gattcacgcg ttgtgtcagg aggccgggaa ccccgatctt
     8401 ggagagagaa cactcgagag cttcactcgc cttttagaag atcctactac cttaaatatc
     8461 aaaggagggg ccagccctac cattctactc aaggatgcta tcagaaaggc tctgtacgac
     8521 gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa tcctgctgtc caagacccat
     8581 agagataact ttatactctt tttaaaatct gttgagcctc tgttccctcg atttctcagt
     8641 gagctcttca gttcgtcttt cttgggaata ccggagtcaa tcattggact gatacaaaac
     8701 tcccggacaa taagaaggca gtttagaaag agtctctcaa gaactttaga agagtccttc
     8761 tacaactcag agatccacgg gattaatcgg ataacccaga cacctcaaag ggtcggaagg
     8821 gtgtggcctt gctcttcaga gagggcagat ctacttaggg agatctcttg ggggaggaaa
     8881 gtggtaggca cgacagttcc tcacccttcc gagatgttgg agttgtttcc caaatcctcc
     8941 atttcctgca cttgtggagc aacaggggga ggcaatccta gagtctctgt atcagtactc
     9001 ccgtccttcg atcagtcatt tttctcacgg ggccccctaa agggatactt gggctcgtcc
     9061 acctccatgt caacccagct attccatgca tgggaaaaag tcactaatgt tcatgtggtg
     9121 aaaagggctc tatcgttaaa agaatctata aactggttca tcaataggaa ttccaatttg
     9181 gctcaaactc taattggaaa catcatgtct ctgacaggcc ctgatttccc tctagaagag
     9241 gcccctgttt tcaaacggac agggtcagcc ttgcataggt tcaagtctgc cagatacagc
     9301 gaaggagggt attcttctgt ttgccctaac cttctctccc atatctctgt tagtacagac
     9361 actatgtctg atttgaccca aaacgggaag aactatgatt tcatgtttca gccattgatg
     9421 ctttatgcgc aaacatggac atcggaactg gtacagaggg atacaagact tagagactcc
     9481 acgtttcact ggcaccttcg gtgcaacaga tgtgtgaggc ccattgatga tataacactg
     9541 gaaacttctc agatcttcga gttcccggat gtgtcaaaaa ggatatccag gatggtttct
     9601 ggagctgtcc ctcaatttca gaagcttcct gatatccgtc taagaccagg tgattttgaa
     9661 tccctaagtg gtagagaaaa gtctcgccat atagggtcag ctcaggggct cttatactca
     9721 atcttagtag caattcacga ctcaggatac aatgatggga ccatcttccc tgtcaacata
     9781 tacggcaaag tttcccccag agactatttg agagggcttg caagagggat cttgataggg
     9841 tcctcgattt gcttcttgac acgaatgaca aatattaaca ttaacagacc tcttgaattg
     9901 atctcagggg taatttccta tattctcctg aggctggata atcatccctc tctgtatata
     9961 atgcttagag aaccgtctct tagaggagaa atattctcta tccctcagaa aatccccgcc
    10021 gcttacccaa ccactatgag agaaggcaac agatcgattt tgtgttacct ccaacacgtg
    10081 ctacgctatg agcgagaggc aatcacggcg tccccggaga atgactggct gtggatcttc
    10141 tcagacttca gaagtgtgaa aatgacgtac ttgaccctca ttacctacca gtctcacctc
    10201 ctactccaga gggttgagag aaacttgtct aagagtatga gagctactct gcgacaaatg
    10261 agttccttaa tgaggcaagt gctgggtggg cacggagaag ataccttgga gtcagacgat
    10321 gacattcaac gattactaaa agactctttg cgaaagacaa ggtgggtgga tcaagaggtg
    10381 cgccatgcag ctagaaccat gaatggagat tacagccccg acaagaaagt atcccacaag
    10441 gcaggatgtt cagaatgggt ctgctctgct caacagattg ccgtctccac ctcagccaac
    10501 ccggcccctg tctcagagct tgacattagg gccctctcta agaggtttca aaaccccttg
    10561 atctcgggcc tgagagtggt tcagtgggca accggtgccc attataagct taagcctatt
    10621 ctagatgatc taaatgtttt cccatctctc tgtcttgtaa ttggagacgg gtcaggggga
    10681 atatcaaggg cagttctcaa catgtttcca gattctaagc ttgtgttcaa cagcctattg
    10741 gaggtgaatg atctgatggc ttccggaaca catccactgc ctccttcagc aatcatgagt
    10801 ggaggagatg acatcatctc cagagtgata gactttgact caatatggga gaaaccgtcc
    10861 gacctgagga actcggccac ctggagatac ttccagtcgg ttcaaaaaca ggtcaacatg
    10921 tcgtatgacc tcattatttg tgatgcagaa gttactgata ttgcatctat caaccggata
    10981 actctgttga tgtctgattt cgcattgtct atagatggac cactttatct ggtcttcaaa
    11041 acttacggga ctatgctagt aaacccggac tataaagcta ttcaacatct gtcaagagcg
    11101 ttcccttcgg tcacagggtt tgtaacccaa gtaacttcat ccttttcttc tgagctatac
    11161 ctccggttct ctaaacgagg aaagtttttc agggacgccg agtacttgac ctcttccacc
    11221 cttcgagaga tgagccttgt gttgttcaat tgcagcagcc ccaaaagtga gatgcagaga
    11281 gctcgttcct taaactatca agacctggta aggggatttc ctgaagagat catatcaaat
    11341 ccttacaacg aaatgatcat aactctgatt gacaatgatg tagagtcctt cctagtccac
    11401 aagatggtgg atgatcttga gctacagagg ggaactctgt ctaaagtggc tatcattata
    11461 tccatcatga tcgttttttc caatagagtc ttcaacattt ccaaaccttt gactgacccc
    11521 ttgttctacc ccccatctga tcctaaaatc ctgaggcact tcaacatatg ttgcagtact
    11581 atgatgtatc tatctaccgc tttaggcgac gtccctaact tcgcaagact tcatgacctg
    11641 tataacagac ctataacttg ttacttcaga aaacaagtta ttcgagggaa tatttatcta
    11701 tcttggagtt ggtccgatga taccccagtg ttcaagagag tagcctgtaa ttctagcttg
    11761 agtctgtcat ctcactggat caggttgatc tacaagatag tgaagactac cagactcatt
    11821 ggcagcataa aagacctatc aggagaggta gaacgacatc ttcatgggta taacagatgg
    11881 atcaccctcg aggatatccg atctagatca tccctactag attacagttg tttgtaagcc
    11941 ggatattacc gaaagcctgt gcatgctaaa attcttgtat gatgcatctt gaaaaaaaca
    12001 agatcttgaa tccggacctc tggttgtttg attgtttttt ccatctttat tgtttttttg
    12061 ttaagcgtgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg catccgaagg
    12121 aggacgcacg tccactcgga tggctaaggg agggcgggga tccggctgct aacaaagccc
    12181 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
    12241 cctctaaacg ggtcttgagg ggttttttgc tgaaagtcgc gcttggcgta atcatggtca
    12301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    12361 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    12421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    12481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    12541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    12601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    12661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    12721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    12781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    12841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    12901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    12961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    13021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    13081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    13141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    13201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    13261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    13321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    13381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    13441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    13501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    13561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccaccctt ggaggctcca
    13621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    13681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    13741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    13801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    13861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    13921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    13981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    14041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    14101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    14161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    14221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    14281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    14341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    14401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
    14461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    14521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    14581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    14641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    14701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    14761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    14821 ccgatttcgg cctattggtt aaaaaaatga gctgatttaa caaaaattta acgcgaattt
    14881 taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa
    14941 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    15001 aggcgattaa gttgggtaac gcgagggttt tcccagtcac gacgttgtaa aacgacggcc
    15061 agtgagcgcg cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    15121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    15181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    15241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    15301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    15361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    15421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
    15481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    15541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
    15601 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
    15661 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
    15721 gattaagcgt ctgatgagtc cgtgaggacg aaacccggcg taccgggtc
//
