LOCUS       sequence-343707-        1590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring 
            the mobility of cytoplasmic particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Parry BR, Surovtsev IV, Cabeen MT, O'Hern CS, Dufresne ER, 
            Jacobs-Wagner C
  TITLE     The bacterial cytoplasm has glass-like properties and is fluidized 
            by metabolic activity.
  JOURNAL   Cell. 2014 Jan 16;156(1-2):183-94. doi: 10.1016/j.cell.2013.11.028. 
            Epub 2013 Dec 19.
  PUBMED    24361104
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2014-01-16"; volume: "156(1-2):183-94. doi"; pages: " 
            10.1016/j.cell.2013.11.028. Epub 2013 Dec 19"
COMMENT     Sequence Label: CJW4617
FEATURES             Location/Qualifiers
     source          1..1590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1..714
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     654..674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    742..766
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1439..1450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1523..1547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgtcgaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcgcgtatgg tcttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaattatgt
      721 ctgcaggcat gcaagctatc aacaagtttg tacaaaaaag caggctcctc cagtgacatg
      781 gtagacggga ttaaactaca gttggacgca tctagacagt gtcatgagtg tcccgtgttg
      841 cagcagaaag tagttgagtt ggaaaaacag attatcatgc agaagtccat tcagtcagat
      901 cctaccccaa tggcgctgca accactgtta tctcagttgc gtgaactgtc tagtgaggtc
      961 acccgactcc agatggagtt aagtcggact cagtccctga atgctcagtt ggaagcggat
     1021 gctaagtcag ctcaagcatg tagtctggat atgtatttga gacaccacac ctgcattaat
     1081 ggtcatacaa aagaagatga actgcttgat gctgtacgtg tcgctccaga tgtgaggaaa
     1141 gaaatcatgg aaaagagggg cgaagtgaga aggggctggt gcgaacgtat ctctaaggaa
     1201 gcggctgcca aatgccaaac tgttattgat gacttgactc agatgaatgg aaagcaggca
     1261 cgagagataa cagaattacg cgagtcagcc gagaattatg agaagcagat tgcggaattg
     1321 gtgggcacta ttactcaaaa ccagatgacg tatcagcaag agctacaagc tttggtagcg
     1381 aagaatgtgg aactggatac gatgaaccaa cgtcaggcta aatcattgcg tattactccc
     1441 tcccttctat cagccactcc tatcgattca gtcgacggcg ctgctgacct gattgatttc
     1501 tccgttccaa ctgatgagct gtacccagct ttcttgtaca aagtggttcg attggctgtt
     1561 ttggcggatg agagaagatt ttcagcctga
//
