LOCUS       sequence-343725-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express overactive YAP (5 LATS 
            phosporylation sites mutated to Ala) under control of the EFS 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: EFSp-GFP-YAP (5SA)
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1935..2146
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2194..2259
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3775..3799)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3841..4415,4416..4418,4419..4427)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4008..4025)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4235..4254
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4428..4430,4431..4433,4434..5144)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4428..4430,4431..4433,4434..5144)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4473..4494)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4734..4753)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5081..5102
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5154..5207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5208..5807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5208..5227)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5704..5724
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5935..6168
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6240..6361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6277..6296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6331..6350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6401..6536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6556..6575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6557..6575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6585..6602)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6585..6601)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6594..6616)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6743..7198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6830..6849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7040..7061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7224..7328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7329..7397,7398..8189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7547..7566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8360..8948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8849..8868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9102..9119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9236..9257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9272..9277,9278..9295,9296..9302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9310..9326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9315..9337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9334..9350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9334..9350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9369..9389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9371..9389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gaggtggctc
     1921 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     1981 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2041 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2101 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagggac cgaatcaccg
     2161 acctctctcc ccagggggat ccaccggagc atggactaca aagaccatga cggtgattat
     2221 aaagatcatg acatcgacta caaggatgac gatgacaaga tggatcccgg gcagcagccg
     2281 ccgcctcaac cggcccccca gggccaaggg cagccgcctt cgcagccccc gcaggggcag
     2341 ggcccgccgt ccggacccgg gcaaccggca cccgcggcga cccaggcggc gccgcaggca
     2401 ccccccgccg ggcatcagat cgtgcacgtc cgcggggacg cggagaccga cctggaggcg
     2461 ctcttcaacg ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt gcccatgagg
     2521 ctccggaagc tgcccgactc cttcttcaag ccgccggagc ccaaatccca ctcccgacag
     2581 gccgctactg atgcaggcac tgcaggagcc ctgactccac agcatgttcg agctcatgcc
     2641 tctccagctt ctctgcagtt gggagctgtt tctcctggga cactgacccc cactggagta
     2701 gtctctggcc cagcagctac acccacagct cagcatcttc gacagtctgc ttttgagata
     2761 cctgatgatg tacctctgcc agcaggttgg gagatggcaa agacatcttc tggtcagaga
     2821 tacttcttaa atcacatcga tcagacaaca acatggcagg accccaggaa ggccatgctg
     2881 tcccagatga acgtcacagc ccccaccagt ccaccagtgc agcagaatat gatgaactcg
     2941 gcttcaggtc ctcttcctga tggatgggaa caagccatga ctcaggatgg agaaatttac
     3001 tatataaacc ataagaacaa gaccacctct tggctagacc caaggcttga ccctcgtttt
     3061 gccatgaacc agagaatcag tcagagtgct ccagtgaaac agccaccacc cctggctccc
     3121 cagagcccac agggaggcgt catgggtggc agcaactcca accagcagca acagatgcga
     3181 ctgcagcaac tgcagatgga gaaggagagg ctgcggctga aacagcaaga actgcttcgg
     3241 caggcaatgc ggaatatcaa tcccagcaca gcaaattctc caaaatgtca ggagttagcc
     3301 ctgcgtagcc agttaccaac actggagcag gatggtggga ctcaaaatcc agtgtcttct
     3361 cccgggatgt ctcaggaatt gagaacaatg acgaccaata gctcagatcc tttccttaac
     3421 agtggcacct atcactctcg agatgaggct acagacagtg gactaagcat gagcagctac
     3481 agtgtccctc gaaccccaga tgacttcctg aacagtgtgg atgagatgga tacaggtgat
     3541 actatcaacc aaagcaccct gccctcacag cagaaccgtt tcccagacta ccttgaagcc
     3601 attcctggga caaatgtgga ccttggaaca ctggaaggag atggaatgaa catagaagga
     3661 gaggagctga tgccaagtct gcaggaagct ttgagttctg acatccttaa tgacatggag
     3721 tctgttttgg ctgccaccaa gctagataaa gaaagctttc ttacatggtt atagacccag
     3781 ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat tccgcccccc
     3841 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     3901 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     3961 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4021 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4081 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4141 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4201 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     4261 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     4321 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     4381 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     4441 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4501 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4561 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4621 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4681 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4741 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4801 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4861 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4921 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4981 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5041 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5101 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcagc ggagagggca
     5161 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggacccatg accgagtaca
     5221 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5281 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5341 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5401 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5461 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5521 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5581 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5641 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5701 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5761 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     5821 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa
     5881 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5941 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6001 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6061 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6121 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6181 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     6241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6361 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     6421 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6481 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6541 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6601 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6661 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6721 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6901 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6961 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7021 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7081 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7141 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     7201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7321 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7381 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7441 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7501 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7561 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7621 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7681 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7741 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7801 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7861 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7921 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7981 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8041 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8101 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8161 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9001 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9061 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9361 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
