LOCUS       sequence-343732-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MDA5 that lacks 2 CARD domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pscALPSpuro-MDA5delta2CARD
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1864..1908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2206..2323
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2460..2867
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5892..6491
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5892..5911)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6388..6408
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6501..7089
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6554..6574)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6972..6983)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7607..7787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7858..8446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7938..7957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8617..9408,9409..9477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9240..9259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9478..9582)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9657..9676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9850..9870)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9855..10184
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10035..10170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10097..10116
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2161 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2281 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2341 cagcagagat ccagtttggt taattaactg cagccccgat aaaataaaag attttattta
     2401 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag
     2461 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
     2521 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     2581 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     2641 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     2701 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     2761 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     2821 acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggggt ctagaagcgc
     2881 tggatccatg gaaatagaaa acctttctca agtagacggc ccgcaggtcg aggaacagct
     2941 tctgagtacg acagtccaac caaatctgga gaaggaggtg tggggaatgg aaaataacag
     3001 ttctgaatca agtttcgccg acagttcagt cgtgtccgaa tctgacacga gcttggcaga
     3061 aggatcagtt agttgtttgg acgagtctct tgggcacaac tccaacatgg gcagcgactc
     3121 tgggactatg ggtagcgact ctgatgagga aaacgtggcc gctcgggcgt ccccagagcc
     3181 tgaacttcag cttcgcccct atcagatgga ggttgcgcag cccgccttgg aaggtaaaaa
     3241 catcattatc tgtttgccta ccggcagtgg aaagacacga gtagcggtct acatcgccaa
     3301 agaccacttg gacaaaaaga aaaaggcgtc agagcctggt aaagtgatag ttttggtcaa
     3361 caaggttctc ttggtggagc agctctttcg gaaggagttt cagccctttc tgaagaaatg
     3421 gtatcgagtg atcggcttgt caggcgatac gcagttgaaa atatcatttc cagaggtagt
     3481 aaagtcctgt gacatcatca tatcaactgc ccaaattctt gaaaattcct tgctgaactt
     3541 ggaaaacggg gaagacgcgg gggtacaact cagcgatttc agtctgatca tcattgatga
     3601 gtgtcatcac acgaataaag aagcagtgta taacaatata atgaggcact atctgatgca
     3661 gaaacttaaa aacaacagac tgaaaaagga gaacaagccc gtcattccat tgccgcaaat
     3721 tcttggcctt acggccagcc ccggcgttgg tggggccacc aaacaagcca aggctgaaga
     3781 acacatcctc aaattgtgcg caaacttgga tgcttttaca ataaagacag tgaaagaaaa
     3841 tcttgaccag ctcaaaaacc agatacaaga accttgcaag aaattcgcga tagccgacgc
     3901 tacccgagaa gatcctttta aagagaagct gctggaaatt atgacgcgga tacagacgta
     3961 ttgtcaaatg agccctatgt ctgactttgg gacgcaacca tatgagcaat gggccataca
     4021 gatggaaaaa aaagctgcga aagagggcaa tcgaaaagag cgcgtctgtg cggagcatct
     4081 caggaagtat aatgaggcgc tgcagatcaa cgatacgatc cgcatgatcg atgcgtacac
     4141 tcacctggaa acgttttaca atgaagagaa agataagaaa tttgcggtca tcgaggacga
     4201 ctcagatgaa ggcggtgacg acgagtattg cgacggagat gaggacgagg atgatcttaa
     4261 gaagcctttg aaactcgatg agactgaccg cttcctcatg acccttttct tcgagaataa
     4321 taaaatgctg aagaggctgg ccgaaaatcc agagtacgag aacgagaagc tcacgaaact
     4381 gcgcaataca ataatggaac aatatacccg cacggaagaa agcgcaagag gcatcatttt
     4441 cactaaaacc aggcagagtg cctacgcctt gagtcagtgg ataaccgaaa atgagaaatt
     4501 cgctgaggtc ggtgtaaaag ctcaccatct tatcggcgct ggacacagtt cagagtttaa
     4561 accgatgact caaaatgagc agaaagaggt aattagtaaa ttccgaacag gaaagattaa
     4621 cttgctgata gccacaactg tggccgaaga gggcttggac ataaaggagt gcaatatcgt
     4681 catacggtat ggtctcgtca ctaacgaaat agcaatggtc caggcccgcg gacgggctag
     4741 agccgatgag agcacttatg tgcttgttgc ccattctgga agcggggtga tcgagcatga
     4801 aaccgtgaat gattttcgcg aaaaaatgat gtacaaggct atacactgtg tgcaaaacat
     4861 gaagccagaa gaatacgccc acaagattct ggaactccaa atgcaaagca ttatggagaa
     4921 aaaaatgaag actaaacgca acattgcgaa acattacaag aacaatccat ctctgattac
     4981 atttctttgt aagaactgct ccgtgctggc ctgttctgga gaggacattc acgtaataga
     5041 gaaaatgcac cacgtgaata tgactcctga gtttaaggag ctttacatag ttcgagagaa
     5101 caaggcgctc caaaagaagt gtgcagatta tcaaataaac ggagaaatca tttgtaagtg
     5161 tggtcaagcg tggggaacca tgatggtgca caaaggcctc gatcttccct gcttgaaaat
     5221 aagaaatttt gtagttgtat tcaaaaacaa ctccactaaa aaacaatata agaaatgggt
     5281 agaactgcca attacgttcc ctaacttgga ctatagtgaa tgttgtctgt ttagtgacga
     5341 agattaggcg gccgcccagc acagtggctc gagccgcggg ttaactggcc agaattctcg
     5401 accttgactg gcggcgcgac cttgaggcct gcgttcgcct cagttgcccc ctctgtgcaa
     5461 tggggagacg cgcctcatcg cttgacaacg gccgaagagc cgccgcgctt ccgtctcccg
     5521 cgtgcgcgcg ccatgctgcc cacccccgtt ccgcactgac cctcccccgt gccccgcgtc
     5581 ccgtactgcc gccccgcccc gagtcccatg ccgcagccac cgcgacggag cccgcaggcg
     5641 ggaacctgcc tccgcgcgtt agcgcgcacg cgcgcctcat gtgtcgtccc catcagcgcc
     5701 ggcttccgtc tataggccag atgcactgtc actctggcga agtcgcagac ccgattggcc
     5761 gggacggagg cgcgagaccg ggttgcgggc ggggccgaac gtggtataaa aggggcggga
     5821 ggccaggctc gtgccgtttt gcagacgcca ccgccgagga aaaccgtgta ctattagcca
     5881 cgcgtgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5941 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6001 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6061 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6121 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6181 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6241 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6301 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6361 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6421 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6481 ccggtgcctg agcgatcgct aatcaacctc tggattacaa aatttgtgaa agattgactg
     6541 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6601 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6661 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6721 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6781 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6841 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6901 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6961 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7021 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7081 cctccccgct taatcgcgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7141 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7201 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7261 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7321 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7381 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7441 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7501 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     7561 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     7621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttcat
     7801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9601 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9661 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9721 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     9781 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     9841 acgcgctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9901 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10021 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10081 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    10141 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc gcgtt
//
