LOCUS       sequence-343758-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K
  TITLE     Engineered HaloTag variants for fluorescence lifetime multiplexing.
  JOURNAL   Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 
            10.1038/s41592-021-01341-x.
  PUBMED    34916672
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 
            10.1038/s41592-021-01341-x."
COMMENT     Sequence Label: pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..85)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        637..840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     791..811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    842..860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    863..881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     891..915
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1063..1072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1065..1148,1149..2315)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             2391..3275
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPWGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             3291..3344
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4737..4754)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4743..4967
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5100..5119)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5251..5298
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5306..6328
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5306..6328
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6458..6579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6495..6514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6549..6568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6628..6644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6628..6644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6641..6663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6676..6682,6683..6700,6701..6706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6721..6742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6859..6876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7030..7618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7110..7129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7789..8580,8581..8649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8412..8431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8650..8754)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
       61 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
      121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
      181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      841 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
      901 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      961 cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac cgagctcgga
     1021 tccactagtc cagtgtggtg gaattctgca gatatccagc aaccatggcg gcccccggca
     1081 gcgcccggcg acccctgctg ctgctactgc tgttgctgct gctcggcctc atgcattgtg
     1141 cgtcagcagc aatgtttatg gtgaaaaatg gcaacgggac cgcgtgcata atggccaact
     1201 tctctgctgc cttctcagtg aactacgaca ccaagagtgg ccctaagaac atgacctttg
     1261 acctgccatc agatgccaca gtggtgctca accgcagctc ctgtggaaaa gagaacactt
     1321 ctgaccccag tctcgtgatt gcttttggaa gaggacatac actcactctc aatttcacga
     1381 gaaatgcaac acgttacagc gtccagctca tgagttttgt ttataacttg tcagacacac
     1441 accttttccc caatgcgagc tccaaagaaa tcaagactgt ggaatctata actgacatca
     1501 gggcagatat agataaaaaa tacagatgtg ttagtggcac ccaggtccac atgaacaacg
     1561 tgaccgtaac gctccatgat gccaccatcc aggcgtacct ttccaacagc agcttcagca
     1621 ggggagagac acgctgtgaa caagacaggc cttccccaac cacagcgccc cctgcgccac
     1681 ccagcccctc gccctcaccc gtgcccaaga gcccctctgt ggacaagtac aacgtgagcg
     1741 gcaccaacgg gacctgcctg ctggccagca tggggctgca gctgaacctc acctatgaga
     1801 ggaaggacaa cacgacggtg acaaggcttc tcaacatcaa ccccaacaag acctcggcca
     1861 gcgggagctg cggcgcccac ctggtgactc tggagctgca cagcgagggc accaccgtcc
     1921 tgctcttcca gttcgggatg aatgcaagtt ctagccggtt tttcctacaa ggaatccagt
     1981 tgaatacaat tcttcctgac gccagagacc ccgcctttaa agctgccaac ggctccctgc
     2041 gagcgctgca ggccacagtc ggcaattcct acaagtgcaa cgcggaggag cacgtccgtg
     2101 tcacgaaggc gttttcagtc aatatattca aagtgtgggt ccaggctttc aaggtggaag
     2161 gtggccagtt tggctctgtg gaggagtgtc tgctggacga gaacagcatg ctgatcccca
     2221 tcgctgtggg tggtgccctg gcggggctgg tcctcatcgt cctcatcgcc tacctcgtcg
     2281 gcaggaagag gagtcacgca ggctaccaga ctatcggctc caccggctcc acaggctcca
     2341 ccggcgcggt cgacggtacc gcgggcccgg gatccatcgc caccggatcc gaaatcggta
     2401 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg
     2461 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct
     2521 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc
     2581 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc
     2641 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg
     2701 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta
     2761 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc
     2821 gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga
     2881 acgtttttat cgagggtacg ctgccgtggg gtgtcgtccg cccgctgact gaagtcgaga
     2941 tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc
     3001 caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca
     3061 tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc
     3121 tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg
     3181 acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga
     3241 tcgcgcgctg gctgtctact ctggagattt ccggtggccg cggcagtgga gagggcagag
     3301 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatggtg ggtcggaaca
     3361 gcgccatcgc cgccggtgta tgcggggccc ttttcattgg gtactgcatc tacttcgacc
     3421 gcaaaagacg aagtgacccc aacttcaaga acaggcttcg agaacgaaga aagaaacaga
     3481 agcttgccaa ggagagagct gggctttcca agttacctga ccttaaagat gctgaagctg
     3541 ttcagaagtt cttccttgaa gaaatacagc ttggtgaaga gttactagct caaggtgaat
     3601 atgagaaggg cgtagaccat ctgacaaatg caattgctgt gtgtggacag ccacagcagt
     3661 tactgcaggt cttacagcaa actcttccac caccagtgtt ccagatgctt ctgactaagc
     3721 tcccaacaat tagtcagaga attgtaagtg ctcagagctt ggctgaagat gatgtggaag
     3781 gcggtagcgg ggatccaccg gtcgccaccg agattggcac agggtttccc tttgaccctc
     3841 attacgttga ggtgctgggt gagaggatgc actatgtcga tgtgggaccc cgtgatggca
     3901 cacctgtgct gtttctccat ggcaatccta ccagctccta tgtgtggagg aacatcatac
     3961 cgcatgtggc tccaacgcac cgatgtatcg caccagatct gatcgggatg ggaaagagcg
     4021 acaaacctga tctgggatac ttcttcgatg accacgtgcg cttcatggac gcctttatcg
     4081 aggctttggg gcttgaggag gtagtgctgg tcatccacga ttggggatct gccctggggt
     4141 ttcattgggc gaaacgcaat cctgagaggg ttaagggcat agccttcatg gagttcatac
     4201 ggcccattcc aacatgggac gaatggccgg aatttgctcg ggaaaccttt caagcgttcc
     4261 gcactaccga tgttgggaga aagctcatca tcgaccacaa cgtgttcatc gaaggaactc
     4321 tccggatggg tgtggtacga cccttgactg aagtcgagat ggaccactat agagagccgt
     4381 ttctcaaccc agtggacaga gaacctctgt ggaggttccc caacgaactt cccattgcag
     4441 gcgaaccagc caatatcgtc gccctcgttg aggagtacat ggactggttg catcagtcac
     4501 cagtacccaa gctgttgttc tggggtacac caggagtgct gattcctccc gcagaagcag
     4561 ctcggctggc taagtctctt cccaactgca aagccgtcga cattggccca ggtctgaatc
     4621 tgcttcagga ggataatcct gatctgattg gcagtgagat tgccagatgg ctgtcaaccc
     4681 tggaaatctc cggtaaccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4741 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4801 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4861 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4921 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4981 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5041 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5101 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5161 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5221 acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct
     5281 agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     5341 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     5401 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     5461 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     5521 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     5581 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     5641 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     5701 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     5761 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     5821 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     5881 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     5941 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     6001 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     6061 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     6121 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     6181 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     6241 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     6301 cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc
     6361 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6421 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6601 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8761 tcc
//
