LOCUS       sequence-343776-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MDA5 S88E mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pscALPSpuro-MDA5-S88E
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     243..262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        342..721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     876..896
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             939..1119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1166..1291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1788..2021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2205..2249
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2547..2664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2801..3208
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6851..7450
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6851..6870)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7347..7367
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7460..8048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7513..7533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7931..7942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8566..8746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8817..9405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8897..8916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9576..10367,10368..10436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10199..10218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10437..10541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10616..10635)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(join(10809..10813,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcgcgt tgacattgat tattgactag
      361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      541 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      901 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      961 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1021 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1081 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1141 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1201 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1261 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1321 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1381 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1441 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1501 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1561 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1621 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1681 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1741 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1801 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1861 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1921 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1981 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2041 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2101 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaattaac aattacacaa
     2161 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2221 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2281 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2341 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2401 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2461 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2521 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2581 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2641 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2701 ttaattaact gcagccccga taaaataaaa gattttattt agtctccaga aaaagggggg
     2761 aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc
     2821 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2881 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2941 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     3001 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     3061 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     3121 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     3181 agtcctccga cagactgagt cgcccggggg tctagaagcg ctggatccat gagtaatggt
     3241 tactcaaccg atgagaattt caggtatctg atttcctgtt tcagagcccg agtcaaaatg
     3301 tacatacaag tcgaacctgt gttggattat ctcacttttc tcccagcaga agtgaaggaa
     3361 caaattcaac ggacggtagc aacttcagga aatatgcagg cggttgagct tttactcagc
     3421 acattggaaa agggtgtgtg gcaccttggg tggactagag agtttgtaga ggccctccgc
     3481 cgcactggtg agcccttggc tgcgcgatac atgaacccgg agctgaccga tttgccatca
     3541 ccttcctttg agaacgccca tgatgagtat ctgcaactct tgaaccttct tcaaccgacc
     3601 ttggttgaca aactgttggt acgcgacgta ctggacaaat gtatggagga agaactgctg
     3661 accattgaag acaggaatcg gatcgccgcc gccgagaata acggcaatga atccggcgtc
     3721 agggaattgc tgaaacgcat cgttcaaaag gagaactggt ttagtgcctt tctcaacgtg
     3781 ctgcgccaaa ccggtaataa tgagttggta caagagttga cgggttccga ctgttccgag
     3841 tcaaatgcgg aaatagaaaa cctttctcaa gtagacggcc cgcaggtcga ggaacagctt
     3901 ctgagtacga cagtccaacc aaatctggag aaggaggtgt ggggaatgga aaataacagt
     3961 tctgaatcaa gtttcgccga cagttcagtc gtgtccgaat ctgacacgag cttggcagaa
     4021 ggatcagtta gttgtttgga cgagtctctt gggcacaact ccaacatggg cagcgactct
     4081 gggactatgg gtagcgactc tgatgaggaa aacgtggccg ctcgggcgtc cccagagcct
     4141 gaacttcagc ttcgccccta tcagatggag gttgcgcagc ccgccttgga aggtaaaaac
     4201 atcattatct gtttgcctac cggcagtgga aagacacgag tagcggtcta catcgccaaa
     4261 gaccacttgg acaaaaagaa aaaggcgtca gagcctggta aagtgatagt tttggtcaac
     4321 aaggttctct tggtggagca gctctttcgg aaggagtttc agccctttct gaagaaatgg
     4381 tatcgagtga tcggcttgtc aggcgatacg cagttgaaaa tatcatttcc agaggtagta
     4441 aagtcctgtg acatcatcat atcaactgcc caaattcttg aaaattcctt gctgaacttg
     4501 gaaaacgggg aagacgcggg ggtacaactc agcgatttca gtctgatcat cattgatgag
     4561 tgtcatcaca cgaataaaga agcagtgtat aacaatataa tgaggcacta tctgatgcag
     4621 aaacttaaaa acaacagact gaaaaaggag aacaagcccg tcattccatt gccgcaaatt
     4681 cttggcctta cggccagccc cggcgttggt ggggccacca aacaagccaa ggctgaagaa
     4741 cacatcctca aattgtgcgc aaacttggat gcttttacaa taaagacagt gaaagaaaat
     4801 cttgaccagc tcaaaaacca gatacaagaa ccttgcaaga aattcgcgat agccgacgct
     4861 acccgagaag atccttttaa agagaagctg ctggaaatta tgacgcggat acagacgtat
     4921 tgtcaaatga gccctatgtc tgactttggg acgcaaccat atgagcaatg ggccatacag
     4981 atggaaaaaa aagctgcgaa agagggcaat cgaaaagagc gcgtctgtgc ggagcatctc
     5041 aggaagtata atgaggcgct gcagatcaac gatacgatcc gcatgatcga tgcgtacact
     5101 cacctggaaa cgttttacaa tgaagagaaa gataagaaat ttgcggtcat cgaggacgac
     5161 tcagatgaag gcggtgacga cgagtattgc gacggagatg aggacgagga tgatcttaag
     5221 aagcctttga aactcgatga gactgaccgc ttcctcatga cccttttctt cgagaataat
     5281 aaaatgctga agaggctggc cgaaaatcca gagtacgaga acgagaagct cacgaaactg
     5341 cgcaatacaa taatggaaca atatacccgc acggaagaaa gcgcaagagg catcattttc
     5401 actaaaacca ggcagagtgc ctacgccttg agtcagtgga taaccgaaaa tgagaaattc
     5461 gctgaggtcg gtgtaaaagc tcaccatctt atcggcgctg gacacagttc agagtttaaa
     5521 ccgatgactc aaaatgagca gaaagaggta attagtaaat tccgaacagg aaagattaac
     5581 ttgctgatag ccacaactgt ggccgaagag ggcttggaca taaaggagtg caatatcgtc
     5641 atacggtatg gtctcgtcac taacgaaata gcaatggtcc aggcccgcgg acgggctaga
     5701 gccgatgaga gcacttatgt gcttgttgcc cattctggaa gcggggtgat cgagcatgaa
     5761 accgtgaatg attttcgcga aaaaatgatg tacaaggcta tacactgtgt gcaaaacatg
     5821 aagccagaag aatacgccca caagattctg gaactccaaa tgcaaagcat tatggagaaa
     5881 aaaatgaaga ctaaacgcaa cattgcgaaa cattacaaga acaatccatc tctgattaca
     5941 tttctttgta agaactgctc cgtgctggcc tgttctggag aggacattca cgtaatagag
     6001 aaaatgcacc acgtgaatat gactcctgag tttaaggagc tttacatagt tcgagagaac
     6061 aaggcgctcc aaaagaagtg tgcagattat caaataaacg gagaaatcat ttgtaagtgt
     6121 ggtcaagcgt ggggaaccat gatggtgcac aaaggcctcg atcttccctg cttgaaaata
     6181 agaaattttg tagttgtatt caaaaacaac tccactaaaa aacaatataa gaaatgggta
     6241 gaactgccaa ttacgttccc taacttggac tatagtgaat gttgtctgtt tagtgacgaa
     6301 gattaggcgg ccgcccagca cagtggctcg agccgcgggt taactggcca gaattctcga
     6361 ccttgactgg cggcgcgacc ttgaggcctg cgttcgcctc agttgccccc tctgtgcaat
     6421 ggggagacgc gcctcatcgc ttgacaacgg ccgaagagcc gccgcgcttc cgtctcccgc
     6481 gtgcgcgcgc catgctgccc acccccgttc cgcactgacc ctcccccgtg ccccgcgtcc
     6541 cgtactgccg ccccgccccg agtcccatgc cgcagccacc gcgacggagc ccgcaggcgg
     6601 gaacctgcct ccgcgcgtta gcgcgcacgc gcgcctcatg tgtcgtcccc atcagcgccg
     6661 gcttccgtct ataggccaga tgcactgtca ctctggcgaa gtcgcagacc cgattggccg
     6721 ggacggaggc gcgagaccgg gttgcgggcg gggccgaacg tggtataaaa ggggcgggag
     6781 gccaggctcg tgccgttttg cagacgccac cgccgaggaa aaccgtgtac tattagccac
     6841 gcgtgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6901 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6961 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7021 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7081 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7141 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7201 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7261 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7321 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7381 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7441 cggtgcctga gcgatcgcta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7501 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7561 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7621 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7681 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7741 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7801 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7861 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7921 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7981 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8041 ctccccgctt aatcgcgtcg agacctagaa aaacatggag caatcacaag tagcaataca
     8101 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8161 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8221 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8281 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     8341 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8401 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8461 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     8521 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     8581 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttcatg
     8761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9001 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9241 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9301 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9361 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9421 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9481 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9541 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9601 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9661 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9721 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9781 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9841 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9901 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9961 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10021 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10081 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10141 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10201 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10261 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10321 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10381 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10441 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10501 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10561 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    10621 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    10681 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    10741 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    10801 cgcgctgtgg aat
//
