LOCUS       sequence-343777-        9079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nipah virus V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pscALPSpuro-Nipah V
FEATURES             Location/Qualifiers
     source          1..9079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..74)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        59..388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      239..374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     301..320
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        400..779
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        781..979
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     934..954
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             997..1177
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1224..1349
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1846..2079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2263..2307
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2605..2722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2859..3266
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3298..3307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5175..5774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5175..5194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5671..5691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5784..6372
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5837..5857)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6255..6266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6890..7070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7141..7729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7221..7240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7900..8691,8692..8760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8523..8542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8761..8865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8940..8959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc tgtggaatgt
       61 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      301 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
      361 cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta ttgactagtt
      421 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      481 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      541 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      601 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      661 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      721 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      781 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      841 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      901 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      961 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     1021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1141 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1201 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1261 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1321 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1381 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1441 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1501 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1561 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1621 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1681 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1741 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1801 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1861 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1921 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1981 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     2041 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     2101 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     2161 acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa ttacacaagc
     2221 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2281 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2341 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2401 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2461 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2521 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     2581 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2641 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2701 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2761 aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     2821 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     2881 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     2941 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     3001 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     3061 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     3121 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     3181 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     3241 tcctccgaca gactgagtcg cccgggggtc tagaagcgct ggatccgttt aaacgcggcc
     3301 gccatggata aacttgaact ggtcaacgac ggattgaaca taatagactt tatacaaaaa
     3361 aaccagaagg agattcagaa aacatatggc agaagctcta tacagcaacc ttctataaaa
     3421 gaccagacca aggcctggga ggatttcctc cagtgtacct ctggcgagtc agagcaggtt
     3481 gagggtggga tgagcaagga cgacggagat gtggagcgac gaaatcttga ggatctctca
     3541 agtacgtccc ctaccgatgg caccataggt aagcgagtgt ccaatacccg agactgggcg
     3601 gaaggttccg acgacataca actggaccct gttgtcaccg atgttgtgta ccacgatcat
     3661 ggcggcgagt gtactggcta tgggttcacc agtagtccag aacgaggttg gagcgactac
     3721 accagcggag caaataacgg taatgtttgt ctggtttctg atgcaaaaat gttgtcttac
     3781 gcgccggaaa ttgctgtgtc taaagaagat agggagaccg atttggtgca tcttgagaac
     3841 aagctctcca ctaccggact caatccgacc gctgtgccat ttaccctgag aaatctctcc
     3901 gatcccgcca aagactcacc agtaatcgcc gagcattact acgggttggg ggtcaaagaa
     3961 caaaatgttg gacctcagac tagccggaac gtgaatctcg attctatcaa gctctacaca
     4021 tcagatgatg aagaggcgga tcaactggag tttgaagacg agtttgctgg gtcatctagc
     4081 gaggtcatcg taggaatctc cccagaagat gaggagccct cctccgtggg tggcaagcct
     4141 aacgagagta tcggtagaac gatagagggc caatctataa gagacaacct ccaagctaaa
     4201 gacaacaaat caacggacgt accgggcgct ggaccaaagg acagtgcggt taaggaagag
     4261 cctccccaga aacgacttcc catgcttgcc gaagagtttg agtgtagtgg ctccgaggac
     4321 cctataatcc gagaactgct gaaagagaac tcactcataa actgtcagca gggcaaagac
     4381 gcccaacccc cgtatcactg gagcatcgag cggtccatat ctcccgataa gacagaaata
     4441 gtaaacgggg cagtccagac agccgatcgc caaagacctg gcacacctat gcccaagtcc
     4501 agaggcatac ccataaagaa gggacacaga agagaaatta gtatctgttg ggatgggaag
     4561 cgagcttggg tcgaggagtg gtgtaaccct gcgtgcagca ggataactcc tttgccaaga
     4621 cgacaagaat gtcagtgtgg tgaatgtcca accgagtgct ttcattgtgg ttaagaattc
     4681 tcgaccttga ctggcggcgc gaccttgagg cctgcgttcg cctcagttgc cccctctgtg
     4741 caatggggag acgcgcctca tcgcttgaca acggccgaag agccgccgcg cttccgtctc
     4801 ccgcgtgcgc gcgccatgct gcccaccccc gttccgcact gaccctcccc cgtgccccgc
     4861 gtcccgtact gccgccccgc cccgagtccc atgccgcagc caccgcgacg gagcccgcag
     4921 gcgggaacct gcctccgcgc gttagcgcgc acgcgcgcct catgtgtcgt ccccatcagc
     4981 gccggcttcc gtctataggc cagatgcact gtcactctgg cgaagtcgca gacccgattg
     5041 gccgggacgg aggcgcgaga ccgggttgcg ggcggggccg aacgtggtat aaaaggggcg
     5101 ggaggccagg ctcgtgccgt tttgcagacg ccaccgccga ggaaaaccgt gtactattag
     5161 ccacgcgtgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5221 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5281 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5341 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5401 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5461 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5521 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5581 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5641 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5701 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5761 agcccggtgc ctgagcgatc gctaatcaac ctctggatta caaaatttgt gaaagattga
     5821 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5881 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5941 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6001 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6061 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6121 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6181 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6241 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6301 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6361 ccgcctcccc gcttaatcgc gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     6421 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6481 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6541 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6601 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     6661 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     6721 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6781 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     6841 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     6901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8881 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     8941 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9001 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9061 tgcatgaaga atctgctta
//
