LOCUS       sequence-343786-        3051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial rrnB gene T2 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: prrnBT2_STD
FEATURES             Location/Qualifiers
     source          1..3051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..32)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(186..774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(266..285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(945..1736,1737..1805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1568..1587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1806..1910)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1988..2443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2125..2146)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2337..2356
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(2469..2496)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     2637..2654
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2638..2654
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2661..2680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2661..2679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2741..2768)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        complement(2859..2877)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2860..2877)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2893..2909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2893..2909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      2980..3051
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgaccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
       61 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcatggga taacgcagga
      121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
      961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1081 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1921 cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1981 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     2041 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     2101 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     2161 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca
     2221 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     2281 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     2341 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     2401 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg taacattgta
     2461 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt
     2521 atggcgggcg tcctgcccgc caccctttca gccaaaaaac ttaagaccgc cggtcttgtc
     2581 cactaccttg cagtaatgcg gtggacagga tcggcggttt tcttttctct tctcaatgta
     2641 aaacgacggc cagtgaattg taatacgact cactataggg tgaattccgc ttaggacctt
     2701 agatattgat ctcaaggtct cgtcgcattg tagaaacgca aaaaggccat ccgtcaggat
     2761 ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccacccttt
     2821 cgcgagacca ctcatcgccc aatcactagt gaattcgttc tatagtgtca cctaaatacc
     2881 atagagccgt tggtcatagc tgtttcctgt cggaccaaaa cgaaaaaagg cccccctttc
     2941 gggaggcctc ttttctggaa tttggtaccg agcaggcatc aaataaaacg aaaggctcag
     3001 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct c
//
