LOCUS       sequence-343795-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. Used for expression of 
            transgenes under a chosen (non-CMV) promoter when a GFP tracer is 
            desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   9..111
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     199..349
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    492..613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(503..522)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     557..576
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(752..1462,1463..1465,1466..1468))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(752..1462,1463..1465,1466..1468))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(794..815)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1143..1162
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1402..1423
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1465..1474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1513..1716)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1542..1562)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1717..2020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   5319..5421
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(5510..5527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5681..6269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5761..5780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7101..7895
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7101..7895
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7155..7174)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7765..7784
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(7943..8323)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8005..8026)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8217..8236
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg ataatgaggg
       61 ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc
      121 ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga
      181 cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga
      241 tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa
      301 taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata tttgtctagg
      361 gccgcgggga ctttgaccgt ttacgtggag actcgcccag gtgtttttct caggtgtttt
      421 ccgcgttccg ggtcaaagtt ggcgttttat tattatagtc agtcgagtct agcaagctag
      481 cttgatcgcg ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      541 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      601 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      661 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      721 gttatctaga tccggtggat ctgagtccgg acttgtacag ctcgtccatg ccgagagtga
      781 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      841 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      901 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      961 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     1021 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     1081 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     1141 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     1201 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     1261 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     1321 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     1381 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     1441 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc ggatctgacg
     1501 gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     1561 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     1621 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     1681 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     1741 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     1801 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     1861 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     1921 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     1981 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2041 atgaactaat gaccccgtaa ttgattacta ttaatcacta gcagatctgg taccgtcgac
     2101 gcggccgcga tatcctcgag aagctttcta gaggatctgg gcgtggttaa gggtgggaaa
     2161 gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc
     2221 catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa cgcgcatgcc
     2281 cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc gccccgtcct
     2341 gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt tggagactgc
     2401 agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga ctgactttgc
     2461 tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt
     2521 gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg tttctcagca
     2581 gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc ccaatgcggt
     2641 ttaaaacata aataaaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg
     2701 tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag
     2761 ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg
     2821 cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt
     2881 gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag
     2941 tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg
     3001 ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat
     3061 gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc
     3121 ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac
     3181 gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg
     3241 ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat
     3301 gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat
     3361 ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag
     3421 ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg
     3481 ggagatcagc tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg
     3541 cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc
     3601 atccctgagc aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac
     3661 caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt
     3721 tttcaacggt ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc
     3781 caggcggtcc cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg
     3841 tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc
     3901 agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag
     3961 gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg
     4021 aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag
     4081 tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac
     4141 gaggggcagt gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg
     4201 gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc
     4261 tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct
     4321 ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat
     4381 acagacttga gagggagttt aaacgaattc aatagcttgt tgcatgggcg gcgatataaa
     4441 atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta
     4501 gtcatgctca tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat
     4561 ttttctctca aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac
     4621 atttaaacat tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg
     4681 actacggcca tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc
     4741 gacagctcct cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga
     4801 ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg
     4861 cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac
     4921 acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca
     4981 acatacagcg cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac
     5041 ctattaaaaa aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg
     5101 ccaagtgcag agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa
     5161 aaacacccag aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa
     5221 cttcctcaaa tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact
     5281 acaattccca acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc
     5341 acgccccgcg ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa
     5401 taaggtatat tattgatgat gttaattaac atgcatggat ccatatgcgg tgtgaaatac
     5461 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     5521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6121 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6721 cagttaatag tttgcgcaac gttgttgcca ttgctgcagc catgagatta tcaaaaagga
     6781 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     6841 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     6901 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     6961 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     7021 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     7081 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7141 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7201 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7261 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     7321 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7381 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7441 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7501 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7561 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7621 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7681 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7741 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7801 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7861 tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc
     7921 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7981 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8041 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8101 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8161 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8221 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8281 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga
     8341 tcgaattaat tc
//
