LOCUS       sequence-343796-       16110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-EGFP-FlpO
FEATURES             Location/Qualifiers
     source          1..16110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3321..3330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3327..3329,3330..3332,3333..4046)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3327..3329,3330..3332,3333..4043)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3372..3393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3633..3652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3980..4001
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7539..7550
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9342..9353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12549..12567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12563..12610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12638..12654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12638..12654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12651..12673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12662..12678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12686..12692,12693..12710,12711..12716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12731..12752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12869..12886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13040..13628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13120..13139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13799..14590,14591..14659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14422..14441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14660..14764)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14791..15247
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14878..14897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15088..15109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15373..15395
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15387..15404
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15388..15404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15474..15777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15778..15981
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15932..15952
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16026..16045
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16026..16044
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggcgtac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3361 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3421 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3481 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     3541 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3601 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3661 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3721 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3781 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3841 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3901 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     3961 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4021 tcggcatgga cgagctgtac aagtaagcta gacatgaaaa aaactaacac tcctccggta
     4081 ccggatccca ccatgagcca gttcgacatc ctgtgcaaga ccccccccaa ggtgctggtg
     4141 cggcagttcg tggagagatt cgagaggccc agcggcgaga agatcgccag ctgtgccgcc
     4201 gagctgacct acctgtgctg gatgatcacc cacaacggca ccgccatcaa gagggccacc
     4261 ttcatgagct acaacaccat catcagcaac agcctgagct tcgacatcgt gaacaagagc
     4321 ctgcagttca agtacaagac ccagaaggcc accatcctgg aggccagcct gaagaagctg
     4381 atccccgcct gggagttcac catcatccct tacaacggcc agaagcacca gagcgacatc
     4441 accgacatcg tgtccagcct gcagctgcag ttcgagagca gcgaggaggc cgacaagggc
     4501 aacagccaca gcaagaagat gctgaaggcc ctgctgtccg agggcgagag catctgggag
     4561 atcaccgaga agatcctgaa cagcttcgag tacaccagca ggttcaccaa gaccaagacc
     4621 ctgtaccagt tcctgttcct ggccacattc atcaactgcg gcaggttcag cgacatcaag
     4681 aacgtggacc ccaagagctt caagctggtg cagaacaagt acctgggcgt gatcattcag
     4741 tgcctggtga ccgagaccaa gacaagcgtg tccaggcaca tctacttttt cagcgccaga
     4801 ggcaggatcg accccctggt gtacctggac gagttcctga ggaacagcga gcccgtgctg
     4861 aagagagtga acaggaccgg caacagcagt agcaacaagc aggagtacca gctgctgaag
     4921 gacaacctgg tgcgcagcta caacaaggcc ctgaagaaga acgcccccta ccccatcttc
     4981 gctatcaaga acggccctaa gagccacatc ggcaggcacc tgatgaccag ctttctgagc
     5041 atgaagggcc tgaccgagct gacaaacgtg gtgggcaact ggagcgacaa gagggcctcc
     5101 gccgtggcca ggaccaccta cacccaccag atcaccgcca tccccgacca ctacttcgcc
     5161 ctggtgtcca ggtactacgc ctacgacccc atcagcaagg agatgatcgc cctgaaggac
     5221 gagaccaacc ccatcgagga gtggcagcac atcgagcagc tgaagggcag cgccgagggc
     5281 agcatcagat accccgcctg gaacggcatc atcagccagg aggtgctgga ctacctgagc
     5341 agctacatca acaggcggat ctgagctagc ggcgcctagc cggtcatcct tttgacgatt
     5401 ccagtcccga ggataacctc ctctcgggat tggggggaat ctttggatcc agtagtcctc
     5461 cttgaactcc gtccaacagg gcagattcaa gagtcataag actttcatta atcatttcag
     5521 ttgatcagac atggtcgtgt agattctctt aatacgggag atcttctagc agtttcagtg
     5581 accaacggtg ctttcattct ccaggaactg ataccaaagg ttgtggacag gccaaggggt
     5641 acttcggatg actctgtgct tgggcacaga aagaggtcgt agtgtgcccc ccgatagcgg
     5701 actcgacatg aatcaactaa gaaaggcaat ctgcctccca tgatggacat aagcaatagt
     5761 tcacaaccat cttgcatctc agtgaagtgt acataactat tgagggctgg gtcatctaag
     5821 catttcagtc gagaaaaaaa ctgtagacca aaagaacaac tagcaacact tctcatccag
     5881 agacccatat caagatgcta gatccgggag aggtttatga tgaccctatt gatccaattg
     5941 agtcagaggc tgaacccaga ggaaccccca ctgtccccaa catcttgagg aactccgact
     6001 acaatctcaa ttctcctttg atagaggatc ctgccaaact aatgttagaa tggttgaaga
     6061 cagggaacag accttatcgg atgactttga cagacaattg ctccaggtct tacaaagttt
     6121 tgaaagatta tttcaagaaa gtagatttgg gttctctcaa agtgggcgga actgctgcac
     6181 agtcaatggt ttctctctgg ttgtgtggtg cccactctga atcaaacagg agccggagat
     6241 gtataaccga cttggcccat ttctattcca agtcatcccc catagagaag ctattgaatt
     6301 gtacgctagg aaacagaggc ctgagaatcc caccagaggg ggtgttaaat tgcctcgaga
     6361 gggtcaatta tgacaaggca tttgggaggt atctggccaa cacgtattcc tcttacttgt
     6421 ttttccatgt aatcacctta tacatgaatg ccttagactg ggaagaggaa aagaccatcc
     6481 tggcattatg gaaagatata acctcagtgg ataccgagaa ggacttggtc aaattcaaag
     6541 atcaaatatg gggactgttg attgtgacaa aggactttgt ttactctcag agttcaaact
     6601 gtctttttga cagaaactac acactgatgc taaaggatct tttcttgtct cgattcaact
     6661 ccttaatgat tctgctttct ccccctgagc cccgatactc agatgactta atatctcagc
     6721 tgtgccagct atacatcgct ggggatcaag tcttgtccat gtgtgggaac tccggctatg
     6781 aagtcatcaa aatattggag ccatatgtcg tgaacagttt ggtccagagg gcagagaagt
     6841 ttaggcctct catccacccc ttgggagact ttcctatgtt cataaaagac aaggtgaatc
     6901 aacttgaagg gacttttggt cccagtgcaa agaggttttt tagggtttta gatcaattcg
     6961 acaacataca tgacttagta tttgtgtatg gctgttacag acattggggg cacccctata
     7021 tagattatcg gaagggtctg tcgaaactat atgatcaagt tcacattaag aaagtaatag
     7081 ataagtccta ccaggagtgt ttagcaagtg acttggccag aaggatcctc agatggggat
     7141 ttgacaagta ctccaaatgg tatctagatt cgagattcct tgcccgagac caccccttga
     7201 ctccttatgt caagacccaa acatggccac ccaaacatat agtagacttg gtgggggaca
     7261 catggcataa gctcccgatc acgcagatct ttgaaattcc tgaatcaatg gacccgtcag
     7321 agatactgga tgacaaatca cattctttca ccagaacaag gttagcttct tggctgtcag
     7381 agaaccgagg ggggcctgtt cctagcgaga aggtcattat cacggccctg tctaagccac
     7441 ctgtcaatcc ccgagagttt ttgaaatcta tagacctcgg aggattgcca gatgaggatt
     7501 tgataattgg cctcaaacca aaggaacggg agttgaagat cgagggccga ttctttgctc
     7561 taatgtcatg gaatctaaga ttatattttg tcatcaccga aaagctcttg gccaactaca
     7621 ttttgccact ttttgacgca ctgactatga cagacaacct gaacaaggtg ttcaaaaagt
     7681 tgatcgacag ggtcaccggg caagggcttt tggactattc tagggtcaca tacgcatttc
     7741 acctggacta tgagaaatgg aacaatcatc aaagattgga gtcaacagag gatgtattct
     7801 ctgtcctaga tcaggtgttt ggattgaaga gggtgttttc tagaacacac gagttttttc
     7861 agaagtcctg gatctattat tcagacagat cagacctcat tgggttatgg gaggaccaaa
     7921 tatattgctt ggatatgtct aacggcccaa cctgctggaa tggccaagat ggcgggctag
     7981 agggcttgcg gcagaagggc tggagtctag tcagtttatt aatgatagat agagaatctc
     8041 aaaccaggaa cacaagaacc aagatactag ctcaaggaga caaccaggtt ctgtgtccta
     8101 catacatgtt gtcaccggga ttgtctcaag aggggcttct ctatgagtta gagagcatat
     8161 caaggaatgc actctcaata taccgagcta tcgaggaagg agcatctaag ctggggctga
     8221 tcatcaagaa ggaagagacc atgtgtagtt atgactttct catatatggg aagaccccct
     8281 tatttcgagg caacatattg gtgcctgaat ccaaaagatg ggcccgagtc tcttgcatct
     8341 ctaacgacca aatagtcaac ctcgccaata taatgtcgac agtatccacc aatgcgctga
     8401 cagtggcaca acactctcaa tctctgatca aacctatgag ggattttctg ctcatgtcag
     8461 tacaggcagt tttccactac ctgttgttta gcccaatcct aaaaggcaga gtttataaga
     8521 ttctgagtgc tgaaggggag agctttctcc tagccatgtc gcggataatc tacctagatc
     8581 cttctttggg aggggtgtct ggaatgtctc tcgggaggtt ccatatacgt cagttctcag
     8641 accctgtctc tgaagggttg tcattctgga gagagatctg gttaggctct catgagtcct
     8701 ggattcacgc gttgtgtcag gaggccggga accccgatct tggagagaga acactcgaga
     8761 gcttcactcg ccttttagaa gatcctacta ccttaaatat caaaggaggg gccagcccta
     8821 ccattctact caaggatgct atcagaaagg ctctgtacga cgaggtggac aaggtggaaa
     8881 attcagagtt tcgagaggca atcctgctgt ccaagaccca tagagataac tttatactct
     8941 ttttaaaatc tgttgagcct ctgttccctc gatttctcag tgagctcttc agttcgtctt
     9001 tcttgggaat accggagtca atcattggac tgatacaaaa ctcccggaca ataagaaggc
     9061 agtttagaaa gagtctctca agaactttag aagagtcctt ctacaactca gagatccacg
     9121 ggattaatcg gataacccag acacctcaaa gggtcggaag ggtgtggcct tgctcttcag
     9181 agagggcaga tctacttagg gagatctctt gggggaggaa agtggtaggc acgacagttc
     9241 ctcacccttc cgagatgttg gagttgtttc ccaaatcctc catttcctgc acttgtggag
     9301 caacaggggg aggcaatcct agagtctctg tatcagtact cccgtccttc gatcagtcat
     9361 ttttctcacg gggcccccta aagggatact tgggctcgtc cacctccatg tcaacccagc
     9421 tattccatgc atgggaaaaa gtcactaatg ttcatgtggt gaaaagggct ctatcgttaa
     9481 aagaatctat aaactggttc atcaatagga attccaattt ggctcaaact ctaattggaa
     9541 acatcatgtc tctgacaggc cctgatttcc ctctagaaga ggcccctgtt ttcaaacgga
     9601 cagggtcagc cttgcatagg ttcaagtctg ccagatacag cgaaggaggg tattcttctg
     9661 tttgccctaa ccttctctcc catatctctg ttagtacaga cactatgtct gatttgaccc
     9721 aaaacgggaa gaactatgat ttcatgtttc agccattgat gctttatgcg caaacatgga
     9781 catcggaact ggtacagagg gatacaagac ttagagactc cacgtttcac tggcaccttc
     9841 ggtgcaacag atgtgtgagg cccattgatg atataacact ggaaacttct cagatcttcg
     9901 agttcccgga tgtgtcaaaa aggatatcca ggatggtttc tggagctgtc cctcaatttc
     9961 agaagcttcc tgatatccgt ctaagaccag gtgattttga atccctaagt ggtagagaaa
    10021 agtctcgcca tatagggtca gctcaggggc tcttatactc aatcttagta gcaattcacg
    10081 actcaggata caatgatggg accatcttcc ctgtcaacat atacggcaaa gtttccccca
    10141 gagactattt gagagggctt gcaagaggga tcttgatagg gtcctcgatt tgcttcttga
    10201 cacgaatgac aaatattaac attaacagac ctcttgaatt gatctcaggg gtaatttcct
    10261 atattctcct gaggctggat aatcatccct ctctgtatat aatgcttaga gaaccgtctc
    10321 ttagaggaga aatattctct atccctcaga aaatccccgc cgcttaccca accactatga
    10381 gagaaggcaa cagatcgatt ttgtgttacc tccaacacgt gctacgctat gagcgagagg
    10441 caatcacggc gtccccggag aatgactggc tgtggatctt ctcagacttc agaagtgtga
    10501 aaatgacgta cttgaccctc attacctacc agtctcacct cctactccag agggttgaga
    10561 gaaacttgtc taagagtatg agagctactc tgcgacaaat gagttcctta atgaggcaag
    10621 tgctgggtgg gcacggagaa gataccttgg agtcagacga tgacattcaa cgattactaa
    10681 aagactcttt gcgaaagaca aggtgggtgg atcaagaggt gcgccatgca gctagaacca
    10741 tgaatggaga ttacagcccc gacaagaaag tatcccacaa ggcaggatgt tcagaatggg
    10801 tctgctctgc tcaacagatt gccgtctcca cctcagccaa cccggcccct gtctcagagc
    10861 ttgacattag ggccctctct aagaggtttc aaaacccctt gatctcgggc ctgagagtgg
    10921 ttcagtgggc aaccggtgcc cattataagc ttaagcctat tctagatgat ctaaatgttt
    10981 tcccatctct ctgtcttgta attggagacg ggtcaggggg aatatcaagg gcagttctca
    11041 acatgtttcc agattctaag cttgtgttca acagcctatt ggaggtgaat gatctgatgg
    11101 cttccggaac acatccactg cctccttcag caatcatgag tggaggagat gacatcatct
    11161 ccagagtgat agactttgac tcaatatggg agaaaccgtc cgacctgagg aactcggcca
    11221 cctggagata cttccagtcg gttcaaaaac aggtcaacat gtcgtatgac ctcattattt
    11281 gtgatgcaga agttactgat attgcatcta tcaaccggat aactctgttg atgtctgatt
    11341 tcgcattgtc tatagatgga ccactttatc tggtcttcaa aacttacggg actatgctag
    11401 taaacccgga ctataaagct attcaacatc tgtcaagagc gttcccttcg gtcacagggt
    11461 ttgtaaccca agtaacttca tccttttctt ctgagctata cctccggttc tctaaacgag
    11521 gaaagttttt cagggacgcc gagtacttga cctcttccac ccttcgagag atgagccttg
    11581 tgttgttcaa ttgcagcagc cccaaaagtg agatgcagag agctcgttcc ttaaactatc
    11641 aagacctggt aaggggattt cctgaagaga tcatatcaaa tccttacaac gaaatgatca
    11701 taactctgat tgacaatgat gtagagtcct tcctagtcca caagatggtg gatgatcttg
    11761 agctacagag gggaactctg tctaaagtgg ctatcattat atccatcatg atcgtttttt
    11821 ccaatagagt cttcaacatt tccaaacctt tgactgaccc cttgttctac cccccatctg
    11881 atcctaaaat cctgaggcac ttcaacatat gttgcagtac tatgatgtat ctatctaccg
    11941 ctttaggcga cgtccctaac ttcgcaagac ttcatgacct gtataacaga cctataactt
    12001 gttacttcag aaaacaagtt attcgaggga atatttatct atcttggagt tggtccgatg
    12061 ataccccagt gttcaagaga gtagcctgta attctagctt gagtctgtca tctcactgga
    12121 tcaggttgat ctacaagata gtgaagacta ccagactcat tggcagcata aaagacctat
    12181 caggagaggt agaacgacat cttcatgggt ataacagatg gatcaccctc gaggatatcc
    12241 gatctagatc atccctacta gattacagtt gtttgtaagc cggatattac cgaaagcctg
    12301 tgcatgctaa aattcttgta tgatgcatct tgaaaaaaac aagatcttga atccggacct
    12361 ctggttgttt gattgttttt tccatcttta ttgttttttt gttaagcgtg ggtcggcatg
    12421 gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgcac gtccactcgg
    12481 atggctaagg gagggcgggg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
    12541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
    12601 gggttttttg ctgaaagtcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    12661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    12721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    12781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    12841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    13021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    13081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    13141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    13201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    13261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    13321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    13501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    13741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    13801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    13861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    13921 gtgctgcaat gataccgcga gacccaccct tggaggctcc agatttatca gcaataaacc
    13981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    14041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    14101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    14161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    14221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    14281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    14341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    14401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    14461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    14521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    14581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    14641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    14701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    14761 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
    14821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    14881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    14941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    15001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    15061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    15121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    15181 taaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    15241 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    15301 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    15361 cgcgagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctagttat
    15421 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    15481 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    15541 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    15601 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    15661 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    15721 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    15781 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    15841 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    15901 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    15961 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
    16021 gaaattaata cgactcacta tagggagacc caagctggct agattaagcg tctgatgagt
    16081 ccgtgaggac gaaacccggc gtaccgggtc
//
