LOCUS       sequence-343799-       16620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-tdTomato-iCre
FEATURES             Location/Qualifiers
     source          1..16620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     regulatory      4795..4804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4807..4827
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8049..8060
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9852..9863)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13059..13077)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13073..13120
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13148..13164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13148..13164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13161..13183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13172..13188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13196..13202,13203..13220,13221..13226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13241..13262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13379..13396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13550..14138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13630..13649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14309..15100,15101..15169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14932..14951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15170..15274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15301..15757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15388..15407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15598..15619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15883..15905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15897..15914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15898..15914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15984..16287
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16288..16491
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16442..16462
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16536..16555
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16536..16554
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaataggc tagacatgaa aaaaactaac actcctccgg taccgccacc
     4801 atggtgccca agaagaagag gaaagtctcc aacctgctga ctgtgcacca aaacctgcct
     4861 gccctccctg tggatgccac ctctgatgaa gtcaggaaga acctgatgga catgttcagg
     4921 gacaggcagg ccttctctga acacacctgg aagatgctcc tgtctgtgtg cagatcctgg
     4981 gctgcctggt gcaagctgaa caacaggaaa tggttccctg ctgaacctga ggatgtgagg
     5041 gactacctcc tgtacctgca agccagaggc ctggctgtga agaccatcca acagcacctg
     5101 ggccagctca acatgctgca caggagatct ggcctgcctc gcccttctga ctccaatgct
     5161 gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg atgctgggga gagagccaag
     5221 caggccctgg cctttgaacg cactgacttt gaccaagtca gatccctgat ggagaactct
     5281 gacagatgcc aggacatcag gaacctggcc ttcctgggca ttgcctacaa caccctgctg
     5341 cgcattgccg aaattgccag aatcagagtg aaggacatct cccgcaccga tggtgggaga
     5401 atgctgatcc acattggcag gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc
     5461 ctgtccctgg gggttaccaa gctggtggag agatggatct ctgtgtctgg tgtggctgat
     5521 gaccccaaca actacctgtt ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc
     5581 acctcccaac tgtccacccg ggccctggaa gggatctttg aggccaccca ccgcctgatc
     5641 tatggtgcca aggatgactc tgggcagaga tacctggcct ggtctggcca ctctgccaga
     5701 gtgggtgctg ccagggacat ggccagggct ggtgtgtcca tccctgaaat catgcaggct
     5761 ggtggctgga ccaatgtgaa cattgtgatg aactacatca gaaacctgga ctctgagact
     5821 ggggccatgg tgaggctgct cgaggatggg gacggcagtg gaggatccgg atgagctagc
     5881 ggcgcctagc cggtcatcct tttgacgatt ccagtcccga ggataacctc ctctcgggat
     5941 tggggggaat ctttggatcc agtagtcctc cttgaactcc gtccaacagg gcagattcaa
     6001 gagtcataag actttcatta atcatttcag ttgatcagac atggtcgtgt agattctctt
     6061 aatacgggag atcttctagc agtttcagtg accaacggtg ctttcattct ccaggaactg
     6121 ataccaaagg ttgtggacag gccaaggggt acttcggatg actctgtgct tgggcacaga
     6181 aagaggtcgt agtgtgcccc ccgatagcgg actcgacatg aatcaactaa gaaaggcaat
     6241 ctgcctccca tgatggacat aagcaatagt tcacaaccat cttgcatctc agtgaagtgt
     6301 acataactat tgagggctgg gtcatctaag catttcagtc gagaaaaaaa ctgtagacca
     6361 aaagaacaac tagcaacact tctcatccag agacccatat caagatgcta gatccgggag
     6421 aggtttatga tgaccctatt gatccaattg agtcagaggc tgaacccaga ggaaccccca
     6481 ctgtccccaa catcttgagg aactccgact acaatctcaa ttctcctttg atagaggatc
     6541 ctgccaaact aatgttagaa tggttgaaga cagggaacag accttatcgg atgactttga
     6601 cagacaattg ctccaggtct tacaaagttt tgaaagatta tttcaagaaa gtagatttgg
     6661 gttctctcaa agtgggcgga actgctgcac agtcaatggt ttctctctgg ttgtgtggtg
     6721 cccactctga atcaaacagg agccggagat gtataaccga cttggcccat ttctattcca
     6781 agtcatcccc catagagaag ctattgaatt gtacgctagg aaacagaggc ctgagaatcc
     6841 caccagaggg ggtgttaaat tgcctcgaga gggtcaatta tgacaaggca tttgggaggt
     6901 atctggccaa cacgtattcc tcttacttgt ttttccatgt aatcacctta tacatgaatg
     6961 ccttagactg ggaagaggaa aagaccatcc tggcattatg gaaagatata acctcagtgg
     7021 ataccgagaa ggacttggtc aaattcaaag atcaaatatg gggactgttg attgtgacaa
     7081 aggactttgt ttactctcag agttcaaact gtctttttga cagaaactac acactgatgc
     7141 taaaggatct tttcttgtct cgattcaact ccttaatgat tctgctttct ccccctgagc
     7201 cccgatactc agatgactta atatctcagc tgtgccagct atacatcgct ggggatcaag
     7261 tcttgtccat gtgtgggaac tccggctatg aagtcatcaa aatattggag ccatatgtcg
     7321 tgaacagttt ggtccagagg gcagagaagt ttaggcctct catccacccc ttgggagact
     7381 ttcctatgtt cataaaagac aaggtgaatc aacttgaagg gacttttggt cccagtgcaa
     7441 agaggttttt tagggtttta gatcaattcg acaacataca tgacttagta tttgtgtatg
     7501 gctgttacag acattggggg cacccctata tagattatcg gaagggtctg tcgaaactat
     7561 atgatcaagt tcacattaag aaagtaatag ataagtccta ccaggagtgt ttagcaagtg
     7621 acttggccag aaggatcctc agatggggat ttgacaagta ctccaaatgg tatctagatt
     7681 cgagattcct tgcccgagac caccccttga ctccttatgt caagacccaa acatggccac
     7741 ccaaacatat agtagacttg gtgggggaca catggcataa gctcccgatc acgcagatct
     7801 ttgaaattcc tgaatcaatg gacccgtcag agatactgga tgacaaatca cattctttca
     7861 ccagaacaag gttagcttct tggctgtcag agaaccgagg ggggcctgtt cctagcgaga
     7921 aggtcattat cacggccctg tctaagccac ctgtcaatcc ccgagagttt ttgaaatcta
     7981 tagacctcgg aggattgcca gatgaggatt tgataattgg cctcaaacca aaggaacggg
     8041 agttgaagat cgagggccga ttctttgctc taatgtcatg gaatctaaga ttatattttg
     8101 tcatcaccga aaagctcttg gccaactaca ttttgccact ttttgacgca ctgactatga
     8161 cagacaacct gaacaaggtg ttcaaaaagt tgatcgacag ggtcaccggg caagggcttt
     8221 tggactattc tagggtcaca tacgcatttc acctggacta tgagaaatgg aacaatcatc
     8281 aaagattgga gtcaacagag gatgtattct ctgtcctaga tcaggtgttt ggattgaaga
     8341 gggtgttttc tagaacacac gagttttttc agaagtcctg gatctattat tcagacagat
     8401 cagacctcat tgggttatgg gaggaccaaa tatattgctt ggatatgtct aacggcccaa
     8461 cctgctggaa tggccaagat ggcgggctag agggcttgcg gcagaagggc tggagtctag
     8521 tcagtttatt aatgatagat agagaatctc aaaccaggaa cacaagaacc aagatactag
     8581 ctcaaggaga caaccaggtt ctgtgtccta catacatgtt gtcaccggga ttgtctcaag
     8641 aggggcttct ctatgagtta gagagcatat caaggaatgc actctcaata taccgagcta
     8701 tcgaggaagg agcatctaag ctggggctga tcatcaagaa ggaagagacc atgtgtagtt
     8761 atgactttct catatatggg aagaccccct tatttcgagg caacatattg gtgcctgaat
     8821 ccaaaagatg ggcccgagtc tcttgcatct ctaacgacca aatagtcaac ctcgccaata
     8881 taatgtcgac agtatccacc aatgcgctga cagtggcaca acactctcaa tctctgatca
     8941 aacctatgag ggattttctg ctcatgtcag tacaggcagt tttccactac ctgttgttta
     9001 gcccaatcct aaaaggcaga gtttataaga ttctgagtgc tgaaggggag agctttctcc
     9061 tagccatgtc gcggataatc tacctagatc cttctttggg aggggtgtct ggaatgtctc
     9121 tcgggaggtt ccatatacgt cagttctcag accctgtctc tgaagggttg tcattctgga
     9181 gagagatctg gttaggctct catgagtcct ggattcacgc gttgtgtcag gaggccggga
     9241 accccgatct tggagagaga acactcgaga gcttcactcg ccttttagaa gatcctacta
     9301 ccttaaatat caaaggaggg gccagcccta ccattctact caaggatgct atcagaaagg
     9361 ctctgtacga cgaggtggac aaggtggaaa attcagagtt tcgagaggca atcctgctgt
     9421 ccaagaccca tagagataac tttatactct ttttaaaatc tgttgagcct ctgttccctc
     9481 gatttctcag tgagctcttc agttcgtctt tcttgggaat accggagtca atcattggac
     9541 tgatacaaaa ctcccggaca ataagaaggc agtttagaaa gagtctctca agaactttag
     9601 aagagtcctt ctacaactca gagatccacg ggattaatcg gataacccag acacctcaaa
     9661 gggtcggaag ggtgtggcct tgctcttcag agagggcaga tctacttagg gagatctctt
     9721 gggggaggaa agtggtaggc acgacagttc ctcacccttc cgagatgttg gagttgtttc
     9781 ccaaatcctc catttcctgc acttgtggag caacaggggg aggcaatcct agagtctctg
     9841 tatcagtact cccgtccttc gatcagtcat ttttctcacg gggcccccta aagggatact
     9901 tgggctcgtc cacctccatg tcaacccagc tattccatgc atgggaaaaa gtcactaatg
     9961 ttcatgtggt gaaaagggct ctatcgttaa aagaatctat aaactggttc atcaatagga
    10021 attccaattt ggctcaaact ctaattggaa acatcatgtc tctgacaggc cctgatttcc
    10081 ctctagaaga ggcccctgtt ttcaaacgga cagggtcagc cttgcatagg ttcaagtctg
    10141 ccagatacag cgaaggaggg tattcttctg tttgccctaa ccttctctcc catatctctg
    10201 ttagtacaga cactatgtct gatttgaccc aaaacgggaa gaactatgat ttcatgtttc
    10261 agccattgat gctttatgcg caaacatgga catcggaact ggtacagagg gatacaagac
    10321 ttagagactc cacgtttcac tggcaccttc ggtgcaacag atgtgtgagg cccattgatg
    10381 atataacact ggaaacttct cagatcttcg agttcccgga tgtgtcaaaa aggatatcca
    10441 ggatggtttc tggagctgtc cctcaatttc agaagcttcc tgatatccgt ctaagaccag
    10501 gtgattttga atccctaagt ggtagagaaa agtctcgcca tatagggtca gctcaggggc
    10561 tcttatactc aatcttagta gcaattcacg actcaggata caatgatggg accatcttcc
    10621 ctgtcaacat atacggcaaa gtttccccca gagactattt gagagggctt gcaagaggga
    10681 tcttgatagg gtcctcgatt tgcttcttga cacgaatgac aaatattaac attaacagac
    10741 ctcttgaatt gatctcaggg gtaatttcct atattctcct gaggctggat aatcatccct
    10801 ctctgtatat aatgcttaga gaaccgtctc ttagaggaga aatattctct atccctcaga
    10861 aaatccccgc cgcttaccca accactatga gagaaggcaa cagatcgatt ttgtgttacc
    10921 tccaacacgt gctacgctat gagcgagagg caatcacggc gtccccggag aatgactggc
    10981 tgtggatctt ctcagacttc agaagtgtga aaatgacgta cttgaccctc attacctacc
    11041 agtctcacct cctactccag agggttgaga gaaacttgtc taagagtatg agagctactc
    11101 tgcgacaaat gagttcctta atgaggcaag tgctgggtgg gcacggagaa gataccttgg
    11161 agtcagacga tgacattcaa cgattactaa aagactcttt gcgaaagaca aggtgggtgg
    11221 atcaagaggt gcgccatgca gctagaacca tgaatggaga ttacagcccc gacaagaaag
    11281 tatcccacaa ggcaggatgt tcagaatggg tctgctctgc tcaacagatt gccgtctcca
    11341 cctcagccaa cccggcccct gtctcagagc ttgacattag ggccctctct aagaggtttc
    11401 aaaacccctt gatctcgggc ctgagagtgg ttcagtgggc aaccggtgcc cattataagc
    11461 ttaagcctat tctagatgat ctaaatgttt tcccatctct ctgtcttgta attggagacg
    11521 ggtcaggggg aatatcaagg gcagttctca acatgtttcc agattctaag cttgtgttca
    11581 acagcctatt ggaggtgaat gatctgatgg cttccggaac acatccactg cctccttcag
    11641 caatcatgag tggaggagat gacatcatct ccagagtgat agactttgac tcaatatggg
    11701 agaaaccgtc cgacctgagg aactcggcca cctggagata cttccagtcg gttcaaaaac
    11761 aggtcaacat gtcgtatgac ctcattattt gtgatgcaga agttactgat attgcatcta
    11821 tcaaccggat aactctgttg atgtctgatt tcgcattgtc tatagatgga ccactttatc
    11881 tggtcttcaa aacttacggg actatgctag taaacccgga ctataaagct attcaacatc
    11941 tgtcaagagc gttcccttcg gtcacagggt ttgtaaccca agtaacttca tccttttctt
    12001 ctgagctata cctccggttc tctaaacgag gaaagttttt cagggacgcc gagtacttga
    12061 cctcttccac ccttcgagag atgagccttg tgttgttcaa ttgcagcagc cccaaaagtg
    12121 agatgcagag agctcgttcc ttaaactatc aagacctggt aaggggattt cctgaagaga
    12181 tcatatcaaa tccttacaac gaaatgatca taactctgat tgacaatgat gtagagtcct
    12241 tcctagtcca caagatggtg gatgatcttg agctacagag gggaactctg tctaaagtgg
    12301 ctatcattat atccatcatg atcgtttttt ccaatagagt cttcaacatt tccaaacctt
    12361 tgactgaccc cttgttctac cccccatctg atcctaaaat cctgaggcac ttcaacatat
    12421 gttgcagtac tatgatgtat ctatctaccg ctttaggcga cgtccctaac ttcgcaagac
    12481 ttcatgacct gtataacaga cctataactt gttacttcag aaaacaagtt attcgaggga
    12541 atatttatct atcttggagt tggtccgatg ataccccagt gttcaagaga gtagcctgta
    12601 attctagctt gagtctgtca tctcactgga tcaggttgat ctacaagata gtgaagacta
    12661 ccagactcat tggcagcata aaagacctat caggagaggt agaacgacat cttcatgggt
    12721 ataacagatg gatcaccctc gaggatatcc gatctagatc atccctacta gattacagtt
    12781 gtttgtaagc cggatattac cgaaagcctg tgcatgctaa aattcttgta tgatgcatct
    12841 tgaaaaaaac aagatcttga atccggacct ctggttgttt gattgttttt tccatcttta
    12901 ttgttttttt gttaagcgtg ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg
    12961 gcatccgaag gaggacgcac gtccactcgg atggctaagg gagggcgggg atccggctgc
    13021 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
    13081 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagtcg cgcttggcgt
    13141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    13201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    13261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    13321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    13381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    13441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    13501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    13561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    13621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    13681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    13741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    13801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    14041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    14101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    14161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    14221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    14281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    14341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    14401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccaccct
    14461 tggaggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    14521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    14581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    14641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    14701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    14761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    14821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    14881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    14941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    15001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    15061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    15121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    15181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    15241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    15301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    15361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    15421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    15481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    15541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    15601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    15661 aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta acaaaaattt
    15721 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
    15781 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    15841 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    15901 aaacgacggc cagtgagcgc gcctagttat taatagtaat caattacggg gtcattagtt
    15961 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
    16021 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
    16081 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    16141 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    16201 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    16261 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    16321 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    16381 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    16441 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
    16501 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
    16561 caagctggct agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc
//
