LOCUS       sequence-343803-       16096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-tdTomato-FlpO
FEATURES             Location/Qualifiers
     source          1..16096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4026
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3458..3476)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3726..3745
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3920..3943
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             7525..7536
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9328..9339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12535..12553)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12549..12596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12624..12640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12624..12640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12637..12659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12648..12664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12672..12678,12679..12696,12697..12702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12717..12738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12855..12872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13026..13614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13106..13125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13785..14576,14577..14645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14408..14427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14646..14750)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14777..15233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14864..14883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15074..15095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15359..15381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15373..15390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15374..15390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15460..15763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15764..15967
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15918..15938
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16012..16031
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16012..16030
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     4021 tacaagtaat aggctagaca tgaaaaaaac taacactcct ccggtaccgg atcccaccat
     4081 gagccagttc gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga
     4141 gagattcgag aggcccagcg gcgagaagat cgccagctgt gccgccgagc tgacctacct
     4201 gtgctggatg gtcacccaca acggcaccgc catcaagagg gccaccttca tgagctacaa
     4261 caccatcatc agcaacagcc tgagcttcga catcgtgaac aagagcctgc agttcaagta
     4321 caagacccag aaggccacca tcctggaggc cagcctgaag aagctgatcc ccgcctggga
     4381 gttcaccatc atcccttaca acggccagaa gcaccagagc gacatcaccg acatcgtgtc
     4441 cagcctgcag ctgcagttcg agagcagcga ggaggccgac aagggcaaca gccacagcaa
     4501 gaagatgctg aaggccctgc tgtccgaggg cgagagcatc tgggagatca ccgagaagat
     4561 cctgaacagc ttcgagtaca ccagcaggtt caccaagacc aagaccctgt accagttcct
     4621 gttcctggcc acattcatca actgcggcag gttcagcgac atcaagaacg tggaccccaa
     4681 gagcttcaag ctggtgcaga acaagtacct gggcgtgatc attcagtgcc tggtgaccga
     4741 gaccaagaca agcgtgtcca ggcacatcta ctttttcagc gccagaggca ggatcgaccc
     4801 cctggtgtac ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga gagtgaacag
     4861 gaccggcaac agcagcagca acaagcagga gtaccagctg ctgaaggaca acctggtgcg
     4921 cagctacaac aaggccctga agaagaacgc cccctacccc atcttcgcta tcaagaacgg
     4981 ccctaagagc cacatcggca ggcacctgat gaccagcttt ctgagcatga agggcctgac
     5041 cgagctgaca aacgtggtgg gcaactggag cgacaagagg gcctccgccg tggccaggac
     5101 cacctacacc caccagatca ccgccatccc cgaccactac ttcgccctgg tgtccaggta
     5161 ctacgcctac gaccccatca gcaaggagat gatcgccctg aaggacgaga ccaaccccat
     5221 cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc gagggcagca tcagataccc
     5281 cgcctggaac ggcatcatca gccaggaggt gctggactac ctgagcagct acatcaacag
     5341 gcggatctga gctagcggcg cctagccggt catccttttg acgattccag tcccgaggat
     5401 aacctcctct cgggattggg gggaatcttt ggatccagta gtcctccttg aactccgtcc
     5461 aacagggcag attcaagagt cataagactt tcattaatca tttcagttga tcagacatgg
     5521 tcgtgtagat tctcttaata cgggagatct tctagcagtt tcagtgacca acggtgcttt
     5581 cattctccag gaactgatac caaaggttgt ggacaggcca aggggtactt cggatgactc
     5641 tgtgcttggg cacagaaaga ggtcgtagtg tgccccccga tagcggactc gacatgaatc
     5701 aactaagaaa ggcaatctgc ctcccatgat ggacataagc aatagttcac aaccatcttg
     5761 catctcagtg aagtgtacat aactattgag ggctgggtca tctaagcatt tcagtcgaga
     5821 aaaaaactgt agaccaaaag aacaactagc aacacttctc atccagagac ccatatcaag
     5881 atgctagatc cgggagaggt ttatgatgac cctattgatc caattgagtc agaggctgaa
     5941 cccagaggaa cccccactgt ccccaacatc ttgaggaact ccgactacaa tctcaattct
     6001 cctttgatag aggatcctgc caaactaatg ttagaatggt tgaagacagg gaacagacct
     6061 tatcggatga ctttgacaga caattgctcc aggtcttaca aagttttgaa agattatttc
     6121 aagaaagtag atttgggttc tctcaaagtg ggcggaactg ctgcacagtc aatggtttct
     6181 ctctggttgt gtggtgccca ctctgaatca aacaggagcc ggagatgtat aaccgacttg
     6241 gcccatttct attccaagtc atcccccata gagaagctat tgaattgtac gctaggaaac
     6301 agaggcctga gaatcccacc agagggggtg ttaaattgcc tcgagagggt caattatgac
     6361 aaggcatttg ggaggtatct ggccaacacg tattcctctt acttgttttt ccatgtaatc
     6421 accttataca tgaatgcctt agactgggaa gaggaaaaga ccatcctggc attatggaaa
     6481 gatataacct cagtggatac cgagaaggac ttggtcaaat tcaaagatca aatatgggga
     6541 ctgttgattg tgacaaagga ctttgtttac tctcagagtt caaactgtct ttttgacaga
     6601 aactacacac tgatgctaaa ggatcttttc ttgtctcgat tcaactcctt aatgattctg
     6661 ctttctcccc ctgagccccg atactcagat gacttaatat ctcagctgtg ccagctatac
     6721 atcgctgggg atcaagtctt gtccatgtgt gggaactccg gctatgaagt catcaaaata
     6781 ttggagccat atgtcgtgaa cagtttggtc cagagggcag agaagtttag gcctctcatc
     6841 caccccttgg gagactttcc tatgttcata aaagacaagg tgaatcaact tgaagggact
     6901 tttggtccca gtgcaaagag gttttttagg gttttagatc aattcgacaa catacatgac
     6961 ttagtatttg tgtatggctg ttacagacat tgggggcacc cctatataga ttatcggaag
     7021 ggtctgtcga aactatatga tcaagttcac attaagaaag taatagataa gtcctaccag
     7081 gagtgtttag caagtgactt ggccagaagg atcctcagat ggggatttga caagtactcc
     7141 aaatggtatc tagattcgag attccttgcc cgagaccacc ccttgactcc ttatgtcaag
     7201 acccaaacat ggccacccaa acatatagta gacttggtgg gggacacatg gcataagctc
     7261 ccgatcacgc agatctttga aattcctgaa tcaatggacc cgtcagagat actggatgac
     7321 aaatcacatt ctttcaccag aacaaggtta gcttcttggc tgtcagagaa ccgagggggg
     7381 cctgttccta gcgagaaggt cattatcacg gccctgtcta agccacctgt caatccccga
     7441 gagtttttga aatctataga cctcggagga ttgccagatg aggatttgat aattggcctc
     7501 aaaccaaagg aacgggagtt gaagatcgag ggccgattct ttgctctaat gtcatggaat
     7561 ctaagattat attttgtcat caccgaaaag ctcttggcca actacatttt gccacttttt
     7621 gacgcactga ctatgacaga caacctgaac aaggtgttca aaaagttgat cgacagggtc
     7681 accgggcaag ggcttttgga ctattctagg gtcacatacg catttcacct ggactatgag
     7741 aaatggaaca atcatcaaag attggagtca acagaggatg tattctctgt cctagatcag
     7801 gtgtttggat tgaagagggt gttttctaga acacacgagt tttttcagaa gtcctggatc
     7861 tattattcag acagatcaga cctcattggg ttatgggagg accaaatata ttgcttggat
     7921 atgtctaacg gcccaacctg ctggaatggc caagatggcg ggctagaggg cttgcggcag
     7981 aagggctgga gtctagtcag tttattaatg atagatagag aatctcaaac caggaacaca
     8041 agaaccaaga tactagctca aggagacaac caggttctgt gtcctacata catgttgtca
     8101 ccgggattgt ctcaagaggg gcttctctat gagttagaga gcatatcaag gaatgcactc
     8161 tcaatatacc gagctatcga ggaaggagca tctaagctgg ggctgatcat caagaaggaa
     8221 gagaccatgt gtagttatga ctttctcata tatgggaaga cccccttatt tcgaggcaac
     8281 atattggtgc ctgaatccaa aagatgggcc cgagtctctt gcatctctaa cgaccaaata
     8341 gtcaacctcg ccaatataat gtcgacagta tccaccaatg cgctgacagt ggcacaacac
     8401 tctcaatctc tgatcaaacc tatgagggat tttctgctca tgtcagtaca ggcagttttc
     8461 cactacctgt tgtttagccc aatcctaaaa ggcagagttt ataagattct gagtgctgaa
     8521 ggggagagct ttctcctagc catgtcgcgg ataatctacc tagatccttc tttgggaggg
     8581 gtgtctggaa tgtctctcgg gaggttccat atacgtcagt tctcagaccc tgtctctgaa
     8641 gggttgtcat tctggagaga gatctggtta ggctctcatg agtcctggat tcacgcgttg
     8701 tgtcaggagg ccgggaaccc cgatcttgga gagagaacac tcgagagctt cactcgcctt
     8761 ttagaagatc ctactacctt aaatatcaaa ggaggggcca gccctaccat tctactcaag
     8821 gatgctatca gaaaggctct gtacgacgag gtggacaagg tggaaaattc agagtttcga
     8881 gaggcaatcc tgctgtccaa gacccataga gataacttta tactcttttt aaaatctgtt
     8941 gagcctctgt tccctcgatt tctcagtgag ctcttcagtt cgtctttctt gggaataccg
     9001 gagtcaatca ttggactgat acaaaactcc cggacaataa gaaggcagtt tagaaagagt
     9061 ctctcaagaa ctttagaaga gtccttctac aactcagaga tccacgggat taatcggata
     9121 acccagacac ctcaaagggt cggaagggtg tggccttgct cttcagagag ggcagatcta
     9181 cttagggaga tctcttgggg gaggaaagtg gtaggcacga cagttcctca cccttccgag
     9241 atgttggagt tgtttcccaa atcctccatt tcctgcactt gtggagcaac agggggaggc
     9301 aatcctagag tctctgtatc agtactcccg tccttcgatc agtcattttt ctcacggggc
     9361 cccctaaagg gatacttggg ctcgtccacc tccatgtcaa cccagctatt ccatgcatgg
     9421 gaaaaagtca ctaatgttca tgtggtgaaa agggctctat cgttaaaaga atctataaac
     9481 tggttcatca ataggaattc caatttggct caaactctaa ttggaaacat catgtctctg
     9541 acaggccctg atttccctct agaagaggcc cctgttttca aacggacagg gtcagccttg
     9601 cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtttg ccctaacctt
     9661 ctctcccata tctctgttag tacagacact atgtctgatt tgacccaaaa cgggaagaac
     9721 tatgatttca tgtttcagcc attgatgctt tatgcgcaaa catggacatc ggaactggta
     9781 cagagggata caagacttag agactccacg tttcactggc accttcggtg caacagatgt
     9841 gtgaggccca ttgatgatat aacactggaa acttctcaga tcttcgagtt cccggatgtg
     9901 tcaaaaagga tatccaggat ggtttctgga gctgtccctc aatttcagaa gcttcctgat
     9961 atccgtctaa gaccaggtga ttttgaatcc ctaagtggta gagaaaagtc tcgccatata
    10021 gggtcagctc aggggctctt atactcaatc ttagtagcaa ttcacgactc aggatacaat
    10081 gatgggacca tcttccctgt caacatatac ggcaaagttt cccccagaga ctatttgaga
    10141 gggcttgcaa gagggatctt gatagggtcc tcgatttgct tcttgacacg aatgacaaat
    10201 attaacatta acagacctct tgaattgatc tcaggggtaa tttcctatat tctcctgagg
    10261 ctggataatc atccctctct gtatataatg cttagagaac cgtctcttag aggagaaata
    10321 ttctctatcc ctcagaaaat ccccgccgct tacccaacca ctatgagaga aggcaacaga
    10381 tcgattttgt gttacctcca acacgtgcta cgctatgagc gagaggcaat cacggcgtcc
    10441 ccggagaatg actggctgtg gatcttctca gacttcagaa gtgtgaaaat gacgtacttg
    10501 accctcatta cctaccagtc tcacctccta ctccagaggg ttgagagaaa cttgtctaag
    10561 agtatgagag ctactctgcg acaaatgagt tccttaatga ggcaagtgct gggtgggcac
    10621 ggagaagata ccttggagtc agacgatgac attcaacgat tactaaaaga ctctttgcga
    10681 aagacaaggt gggtggatca agaggtgcgc catgcagcta gaaccatgaa tggagattac
    10741 agccccgaca agaaagtatc ccacaaggca ggatgttcag aatgggtctg ctctgctcaa
    10801 cagattgccg tctccacctc agccaacccg gcccctgtct cagagcttga cattagggcc
    10861 ctctctaaga ggtttcaaaa ccccttgatc tcgggcctga gagtggttca gtgggcaacc
    10921 ggtgcccatt ataagcttaa gcctattcta gatgatctaa atgttttccc atctctctgt
    10981 cttgtaattg gagacgggtc agggggaata tcaagggcag ttctcaacat gtttccagat
    11041 tctaagcttg tgttcaacag cctattggag gtgaatgatc tgatggcttc cggaacacat
    11101 ccactgcctc cttcagcaat catgagtgga ggagatgaca tcatctccag agtgatagac
    11161 tttgactcaa tatgggagaa accgtccgac ctgaggaact cggccacctg gagatacttc
    11221 cagtcggttc aaaaacaggt caacatgtcg tatgacctca ttatttgtga tgcagaagtt
    11281 actgatattg catctatcaa ccggataact ctgttgatgt ctgatttcgc attgtctata
    11341 gatggaccac tttatctggt cttcaaaact tacgggacta tgctagtaaa cccggactat
    11401 aaagctattc aacatctgtc aagagcgttc ccttcggtca cagggtttgt aacccaagta
    11461 acttcatcct tttcttctga gctatacctc cggttctcta aacgaggaaa gtttttcagg
    11521 gacgccgagt acttgacctc ttccaccctt cgagagatga gccttgtgtt gttcaattgc
    11581 agcagcccca aaagtgagat gcagagagct cgttccttaa actatcaaga cctggtaagg
    11641 ggatttcctg aagagatcat atcaaatcct tacaacgaaa tgatcataac tctgattgac
    11701 aatgatgtag agtccttcct agtccacaag atggtggatg atcttgagct acagagggga
    11761 actctgtcta aagtggctat cattatatcc atcatgatcg ttttttccaa tagagtcttc
    11821 aacatttcca aacctttgac tgaccccttg ttctaccccc catctgatcc taaaatcctg
    11881 aggcacttca acatatgttg cagtactatg atgtatctat ctaccgcttt aggcgacgtc
    11941 cctaacttcg caagacttca tgacctgtat aacagaccta taacttgtta cttcagaaaa
    12001 caagttattc gagggaatat ttatctatct tggagttggt ccgatgatac cccagtgttc
    12061 aagagagtag cctgtaattc tagcttgagt ctgtcatctc actggatcag gttgatctac
    12121 aagatagtga agactaccag actcattggc agcataaaag acctatcagg agaggtagaa
    12181 cgacatcttc atgggtataa cagatggatc accctcgagg atatccgatc tagatcatcc
    12241 ctactagatt acagttgttt gtaagccgga tattaccgaa agcctgtgca tgctaaaatt
    12301 cttgtatgat gcatcttgaa aaaaacaaga tcttgaatcc ggacctctgg ttgtttgatt
    12361 gttttttcca tctttattgt ttttttgtta agcgtgggtc ggcatggcat ctccacctcc
    12421 tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc actcggatgg ctaagggagg
    12481 gcggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
    12541 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
    12601 aagtcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12661 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12721 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12781 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    13021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    13081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    13141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    13201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    13261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    13321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    13441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    13501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    13561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    13741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    13801 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    13861 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    13921 ccgcgagacc cacccttgga ggctccagat ttatcagcaa taaaccagcc agccggaagg
    13981 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    14041 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    14101 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    14161 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    14221 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    14281 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    14341 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    14401 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    14461 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    14521 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    14581 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    14641 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    14701 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    14761 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    14821 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    14881 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    14941 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    15001 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    15061 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    15121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct
    15181 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg
    15241 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    15301 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    15361 cagtcacgac gttgtaaaac gacggccagt gagcgcgcct agttattaat agtaatcaat
    15421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    15481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    15541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    15601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    15661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    15721 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    15781 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    15841 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    15901 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    15961 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
    16021 tcactatagg gagacccaag ctggctagat taagcgtctg atgagtccgt gaggacgaaa
    16081 cccggcgtac cgggtc
//
