LOCUS       sequence-343804-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexA activated transgene coexpression of protein calcium sensor and 
            tdTomato under the hsp70 promoter in D. melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  TITLE     GENIE Drosophila calcium sensors
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(127..195,196..987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(345..364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1158..1746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1647..1666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1833..1902
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2127..2145)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2597..2668,3070..3343,3418..4072,4134..4449,4653..4784,
                     4855..5469)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6881..7119
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7066..7087
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7134..7200
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7239..7256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(7296..7304,7305..7319)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             7347..7403
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             8580..8633
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(10780..10798)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
       61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      121 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      181 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      241 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      301 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      361 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      421 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      481 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      541 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      601 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      661 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      721 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      781 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      841 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      901 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      961 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1021 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1801 cacatgttac cgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
     1861 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac
     1921 gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc
     1981 tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt
     2041 gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacaagc
     2101 cgaacatatg ggcgcgccta gtatgtatgt aagttaataa aacccatttt tgcggaaagt
     2161 agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt atctgatcag
     2221 ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca
     2281 ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat
     2341 atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg
     2401 tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc
     2461 ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt
     2521 tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt
     2581 gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa
     2641 acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc
     2701 acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac
     2761 ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg
     2821 gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa
     2881 ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc
     2941 tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg
     3001 gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta
     3061 tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag
     3121 gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc
     3181 ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc
     3241 aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac
     3301 gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca
     3361 gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt
     3421 gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga
     3481 cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg
     3541 ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct
     3601 atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc
     3661 aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc
     3721 aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca
     3781 gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggctctaa
     3841 ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc tttaccgccc
     3901 acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca
     3961 ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg
     4021 gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc
     4081 gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc cagcgtgggt
     4141 gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg
     4201 cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt
     4261 agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga gaagccactg
     4321 gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc
     4381 ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt
     4441 cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa
     4501 caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag tttttttcaa
     4561 tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac tcgcgaatat
     4621 taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct
     4681 ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc atcttcctct
     4741 tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt catgtttaga
     4801 atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc
     4861 acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca
     4921 tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc actggtcttc
     4981 acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc
     5041 ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc
     5101 gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg
     5161 ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
     5221 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
     5281 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
     5341 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
     5401 attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc
     5461 aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc
     5521 atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa
     5581 aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac
     5641 tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg
     5701 tttatcgcta cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg
     5761 ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc
     5821 acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt
     5881 tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc aatagaaatt
     5941 agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata tatttccctc
     6001 acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt tgtgacctgt
     6061 tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg ttccttgtgt
     6121 agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc aaaaataaca
     6181 actataataa taagaataca tttaatttag aaaatgcttg gatttcactg gaactagggc
     6241 gcgcctccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     6301 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     6361 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     6421 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc agggccgcaa
     6481 gcttgcatgc ctgcaggtta ctgtacatcc atacagtaag tactgtacat ccatacagta
     6541 agtactgtac atccatacag taagtactgt acatccatac agtaagtact gtacatccat
     6601 acagtaagcg gagactctag ccctagggca tgcctgcagg ttactgtaca tccatacagt
     6661 aagtactgta catccataca gtaagtactg tacatccata cagtaagcgg agactctagc
     6721 gctagcgcat gcctgcaggt tactgtacat ccatacagta agtactgtac atccatacag
     6781 taagtactgt acatccatac agtaagtact gtacatccat acagtaagta ctgtacatcc
     6841 atacagtaag cggagactct agcactagtg acgtcgagcg ccggagtata aatagaggcg
     6901 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     6961 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
     7021 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
     7081 tgccaagaag taattattga atacaagaag agaactctga atagatctaa aaggtaggtt
     7141 caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag
     7201 gcggccgcaa cttaaaaaaa aaaatcaaaa tggggtcgca tcatcatcac caccacggca
     7261 tggcctcgat gaccggcggc cagcagatgg gccgagatct gtacgacgat gacgataaag
     7321 atctggcgac catggttgat tcatcgcgac gcaagtggaa taaaacaggc catgcagtgc
     7381 gagcgatagg ccgcctgagc agcctggaga acgtttacat caaagcggat aagcagaaaa
     7441 acggcatcaa agcaaacttt aaaatccgtc acaatattga ggatggtggt gtccagctgg
     7501 cctatcacta tcaacagaac accccgatcg gagatggccc cgttctgcta ccagacaacc
     7561 attacctcag cgtgcagtcg aaattgtcaa aggacccaaa cgagaaaagg gatcacatgg
     7621 tgctgttgga gttcgtgacc gcggctggca ttactttggg gatggatgag ctgtataagg
     7681 gaggaacggg tggaagcatg gtctccaagg gagaggagtt gttcaccgga gtagttccca
     7741 ttttagtaga gcttgatgga gatgtaaacg ggcataagtt ctccgtgtcc ggagaaggcg
     7801 agggcgacgc cacctatggc aaactcacgt tgaaattcat ttgcacgacg ggcaagctgc
     7861 cagtcccttg gcccacactg gttacgaccc tcacttatgg cgtccagtgc ttcagccgtt
     7921 atcctgacca catgaagcag catgatttct ttaagagtgc catgcccgag ggctatattc
     7981 aggagagaac aatttttttt aaggatgatg gaaactataa aacccgtgct gaggtcaagt
     8041 tcgagggaga cacactggtg aaccgtatcg agctgaaagg tatcgatttt aaggaggacg
     8101 gtaatatcct gggccacaag cttgagtaca atccgccgga ccagttaact gaggaacaga
     8161 tcgcggaatt caaagagttg ttcagtctct tcgataagga cggcgatggt acgatcacaa
     8221 ccaaggagct tggcacagtc atgcgtagtc tcggtcagaa tccgaccgag gccgagctgc
     8281 aggacatgat caatgaagtt gatgccgatg gcgacggtac cattgacttc ccggagtttc
     8341 tcacgatgta tgctcggaaa atgaagtaca gggatacaga ggaggaaatt cgagaggctt
     8401 tcggcgtttt cgataaagac ggcaacggtt atatttcggc ggcagagctg cgtcacgtta
     8461 tgacaaatct gggtgaaaag ctaacagatg aggaagtcga tgagatgatc agagaggcag
     8521 acattgacgg tgacggacag gtgaactacg aggagtttgt tcaaatgatg acggccaagg
     8581 agggtcgcgg atcgctgctg acctgcggcg acgtggagga aaaccccggc cccgttagca
     8641 agggtgaaga ggtgattaaa gaattcatgc gttttaaagt acgcatggag ggtagcatga
     8701 acggtcatga gtttgagatc gagggcgaag gcgaaggtcg tccgtacgag ggtacgcaga
     8761 ccgctaaact gaaagtaacc aaaggcggtc ctctgccgtt cgcgtgggac atcctgtccc
     8821 cgcagtttat gtatggttcc aaggcctacg tcaaacaccc agccgatatc ccggactaca
     8881 agaagctgtc tttcccggag ggtttcaagt gggagcgtgt aatgaacttt gaggacggtg
     8941 gcctggttac cgtcacccag gatagctctc tgcaagatgg caccctgatc tacaaggtga
     9001 agatgcgtgg tacgaacttt ccgccggatg gcccggtgat gcagaaaaag acgatgggat
     9061 gggaagcatc taccgagcgc ctgtatccgc gtgacggtgt tctgaaaggt gagatccacc
     9121 aggcgctgaa actgaaggac ggcggtcact acctggttga atttaagacg atctatatgg
     9181 ctaaaaagcc ggttcaactg ccaggttact actacgtgga caccaaactg gatatcacgt
     9241 ctcacaacga agattatacc atcgtcgaac agtatgaacg tagcgagggc cgtcaccacc
     9301 tgttcctggg ccacggtact ggttctacgg gttctggttc ctctggcact gcgtcttccg
     9361 aagacaacaa catggcggtg atcaaagagt tcatgaggtt caaagtacgt atggaaggtt
     9421 ctatgaacgg ccatgagttc gagattgaag gtgagggcga gggtcgccca tacgagggca
     9481 ctcagaccgc aaagctgaaa gttaccaaag gtggcccact gccttttgcg tgggatatcc
     9541 tgtctccgca gttcatgtac ggttccaaag cctacgtaaa acacccggca gacatcccgg
     9601 attacaaaaa gctgtccttc ccagagggct tcaaatggga acgtgttatg aatttcgaag
     9661 atggcggcct ggtcactgtt acgcaggaca gcagcctgca agacggtact ctgatttaca
     9721 aagtgaaaat gcgtggcact aacttcccgc ctgacggtcc agtaatgcag aagaaaacta
     9781 tgggctggga agctagcacc gaacgtctgt acccgcgcga cggtgtgctg aaaggcgaga
     9841 ttcaccaggc cctgaagctg aaggatggtg gtcactatct ggttgagttc aaaactatct
     9901 acatggcaaa aaagcctgta caactgccgg gctattacta cgttgacact aaactggaca
     9961 tcacctccca caacgaggat tatactattg ttgaacagta cgagcgttct gaaggtcgcc
    10021 atcatctgtt tctgtatggt atggacgaac tgtataaata aggtgtacaa atctagaatg
    10081 aatcgttttt aaaataacaa atcaattgtt ttataatatt cgtacgattc tttgattatg
    10141 taataaaatg tgatcattag gaagattacg aaaaatataa aaaatatgag ttctgtgtgt
    10201 ataacaaatg ctgtaaacgc cacaattgtg tttgttgcaa ataaacccat gattatttga
    10261 ttaaaattgt tgttttcttt gttcatagac aatagtgtgt tttgcctaaa cgtgtactgc
    10321 ataaactcca tgcgagtgta tagcgagcta gtggctaacg cttgccccac caaagtagat
    10381 tcgtcaaaat cctcaatttc atcaccctcc tccaagttta acatttggcc gtcggaatta
    10441 acttctaaag atgccacata atctaataaa tgaaatagag attcaaacgt ggcgtcatcg
    10501 tccgtttcga ccatttccga aaagaactcg ggcataaact ctatgatttc tctggacgtg
    10561 gtgttgtcga aactctcaaa gtacgcagtc aggaacgtgc gcgacatgtc gtcgggaaac
    10621 tcgcgcggaa acatgttgtt gtaaccgaac gggtcccata gcgccaaaac caaatctgcc
    10681 agcgtcaata gaatgagcac gatgccgaca atggagctgg cttggatagc gattcgagtt
    10741 aacggccggc cgtttaaacg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
    10801 tataggttaa tgtcatgata ataatggttt cttagactca ggt
//
