LOCUS       sequence-343807-       14748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 14748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-E2_Crimson
FEATURES             Location/Qualifiers
     source          1..14748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4002
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     3723..3742
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6177..6188
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(7980..7991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11187..11205)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11201..11248
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11276..11292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11276..11292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11289..11311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11300..11316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11324..11330,11331..11348,11349..11354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11369..11390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11507..11524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11678..12266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11758..11777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12437..13228,13229..13297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13060..13079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13298..13402)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13429..13885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13516..13535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13726..13747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14011..14033
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14025..14042
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14026..14042
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14112..14415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14416..14619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14570..14590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     14664..14683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14664..14682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggat agcactgaga acgtcatcaa gcccttcatg
     3361 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgtg
     3421 ggcgagggca agccctacga gggcacccag accgccaagc tgcaagtgac caagggcggc
     3481 cccctgccct tcgcctggga catcctgtcc ccccagttct tctacggctc caaggcgtac
     3541 atcaagcacc ccgccgacat ccccgactac ctcaagcagt ccttccccga gggcttcaag
     3601 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3661 ctgcaggacg gcaccctcat ctaccacgtg aagttcatcg gcgtgaactt cccctccgac
     3721 ggccccgtaa tgcagaagaa gactctgggc tgggagccct ccactgagcg caactacccc
     3781 cgcgacggcg tgctgaaggg cgagaaccac atggcgctga agctgaaggg cggcggccac
     3841 tacctgtgtg agttcaagtc catctacatg gccaagaagc ccgtgaagct gcccggctac
     3901 cactacgtgg actacaagct cgacatcacc tcccacaacg aggactacac cgtggtggag
     3961 cagtacgagc gcgccgaggc ccgccaccac ctgttccagt aggctagcgg cgcctagccg
     4021 gtcatccttt tgacgattcc agtcccgagg ataacctcct ctcgggattg gggggaatct
     4081 ttggatccag tagtcctcct tgaactccgt ccaacagggc agattcaaga gtcataagac
     4141 tttcattaat catttcagtt gatcagacat ggtcgtgtag attctcttaa tacgggagat
     4201 cttctagcag tttcagtgac caacggtgct ttcattctcc aggaactgat accaaaggtt
     4261 gtggacaggc caaggggtac ttcggatgac tctgtgcttg ggcacagaaa gaggtcgtag
     4321 tgtgcccccc gatagcggac tcgacatgaa tcaactaaga aaggcaatct gcctcccatg
     4381 atggacataa gcaatagttc acaaccatct tgcatctcag tgaagtgtac ataactattg
     4441 agggctgggt catctaagca tttcagtcga gaaaaaaact gtagaccaaa agaacaacta
     4501 gcaacacttc tcatccagag acccatatca agatgctaga tccgggagag gtttatgatg
     4561 accctattga tccaattgag tcagaggctg aacccagagg aacccccact gtccccaaca
     4621 tcttgaggaa ctccgactac aatctcaatt ctcctttgat agaggatcct gccaaactaa
     4681 tgttagaatg gttgaagaca gggaacagac cttatcggat gactttgaca gacaattgct
     4741 ccaggtctta caaagttttg aaagattatt tcaagaaagt agatttgggt tctctcaaag
     4801 tgggcggaac tgctgcacag tcaatggttt ctctctggtt gtgtggtgcc cactctgaat
     4861 caaacaggag ccggagatgt ataaccgact tggcccattt ctattccaag tcatccccca
     4921 tagagaagct attgaattgt acgctaggaa acagaggcct gagaatccca ccagaggggg
     4981 tgttaaattg cctcgagagg gtcaattatg acaaggcatt tgggaggtat ctggccaaca
     5041 cgtattcctc ttacttgttt ttccatgtaa tcaccttata catgaatgcc ttagactggg
     5101 aagaggaaaa gaccatcctg gcattatgga aagatataac ctcagtggat accgagaagg
     5161 acttggtcaa attcaaagat caaatatggg gactgttgat tgtgacaaag gactttgttt
     5221 actctcagag ttcaaactgt ctttttgaca gaaactacac actgatgcta aaggatcttt
     5281 tcttgtctcg attcaactcc ttaatgattc tgctttctcc ccctgagccc cgatactcag
     5341 atgacttaat atctcagctg tgccagctat acatcgctgg ggatcaagtc ttgtccatgt
     5401 gtgggaactc cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aacagtttgg
     5461 tccagagggc agagaagttt aggcctctca tccacccctt gggagacttt cctatgttca
     5521 taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc cagtgcaaag aggtttttta
     5581 gggttttaga tcaattcgac aacatacatg acttagtatt tgtgtatggc tgttacagac
     5641 attgggggca cccctatata gattatcgga agggtctgtc gaaactatat gatcaagttc
     5701 acattaagaa agtaatagat aagtcctacc aggagtgttt agcaagtgac ttggccagaa
     5761 ggatcctcag atggggattt gacaagtact ccaaatggta tctagattcg agattccttg
     5821 cccgagacca ccccttgact ccttatgtca agacccaaac atggccaccc aaacatatag
     5881 tagacttggt gggggacaca tggcataagc tcccgatcac gcagatcttt gaaattcctg
     5941 aatcaatgga cccgtcagag atactggatg acaaatcaca ttctttcacc agaacaaggt
     6001 tagcttcttg gctgtcagag aaccgagggg ggcctgttcc tagcgagaag gtcattatca
     6061 cggccctgtc taagccacct gtcaatcccc gagagttttt gaaatctata gacctcggag
     6121 gattgccaga tgaggatttg ataattggcc tcaaaccaaa ggaacgggag ttgaagatcg
     6181 agggccgatt ctttgctcta atgtcatgga atctaagatt atattttgtc atcaccgaaa
     6241 agctcttggc caactacatt ttgccacttt ttgacgcact gactatgaca gacaacctga
     6301 acaaggtgtt caaaaagttg atcgacaggg tcaccgggca agggcttttg gactattcta
     6361 gggtcacata cgcatttcac ctggactatg agaaatggaa caatcatcaa agattggagt
     6421 caacagagga tgtattctct gtcctagatc aggtgtttgg attgaagagg gtgttttcta
     6481 gaacacacga gttttttcag aagtcctgga tctattattc agacagatca gacctcattg
     6541 ggttatggga ggaccaaata tattgcttgg atatgtctaa cggcccaacc tgctggaatg
     6601 gccaagatgg cgggctagag ggcttgcggc agaagggctg gagtctagtc agtttattaa
     6661 tgatagatag agaatctcaa accaggaaca caagaaccaa gatactagct caaggagaca
     6721 accaggttct gtgtcctaca tacatgttgt caccgggatt gtctcaagag gggcttctct
     6781 atgagttaga gagcatatca aggaatgcac tctcaatata ccgagctatc gaggaaggag
     6841 catctaagct ggggctgatc atcaagaagg aagagaccat gtgtagttat gactttctca
     6901 tatatgggaa gaccccctta tttcgaggca acatattggt gcctgaatcc aaaagatggg
     6961 cccgagtctc ttgcatctct aacgaccaaa tagtcaacct cgccaatata atgtcgacag
     7021 tatccaccaa tgcgctgaca gtggcacaac actctcaatc tctgatcaaa cctatgaggg
     7081 attttctgct catgtcagta caggcagttt tccactacct gttgtttagc ccaatcctaa
     7141 aaggcagagt ttataagatt ctgagtgctg aaggggagag ctttctccta gccatgtcgc
     7201 ggataatcta cctagatcct tctttgggag gggtgtctgg aatgtctctc gggaggttcc
     7261 atatacgtca gttctcagac cctgtctctg aagggttgtc attctggaga gagatctggt
     7321 taggctctca tgagtcctgg attcacgcgt tgtgtcagga ggccgggaac cccgatcttg
     7381 gagagagaac actcgagagc ttcactcgcc ttttagaaga tcctactacc ttaaatatca
     7441 aaggaggggc cagccctacc attctactca aggatgctat cagaaaggct ctgtacgacg
     7501 aggtggacaa ggtggaaaat tcagagtttc gagaggcaat cctgctgtcc aagacccata
     7561 gagataactt tatactcttt ttaaaatctg ttgagcctct gttccctcga tttctcagtg
     7621 agctcttcag ttcgtctttc ttgggaatac cggagtcaat cattggactg atacaaaact
     7681 cccggacaat aagaaggcag tttagaaaga gtctctcaag aactttagaa gagtccttct
     7741 acaactcaga gatccacggg attaatcgga taacccagac acctcaaagg gtcggaaggg
     7801 tgtggccttg ctcttcagag agggcagatc tacttaggga gatctcttgg gggaggaaag
     7861 tggtaggcac gacagttcct cacccttccg agatgttgga gttgtttccc aaatcctcca
     7921 tttcctgcac ttgtggagca acagggggag gcaatcctag agtctctgta tcagtactcc
     7981 cgtccttcga tcagtcattt ttctcacggg gccccctaaa gggatacttg ggctcgtcca
     8041 cctccatgtc aacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga
     8101 aaagggctct atcgttaaaa gaatctataa actggttcat caataggaat tccaatttgg
     8161 ctcaaactct aattggaaac atcatgtctc tgacaggccc tgatttccct ctagaagagg
     8221 cccctgtttt caaacggaca gggtcagcct tgcataggtt caagtctgcc agatacagcg
     8281 aaggagggta ttcttctgtt tgccctaacc ttctctccca tatctctgtt agtacagaca
     8341 ctatgtctga tttgacccaa aacgggaaga actatgattt catgtttcag ccattgatgc
     8401 tttatgcgca aacatggaca tcggaactgg tacagaggga tacaagactt agagactcca
     8461 cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc cattgatgat ataacactgg
     8521 aaacttctca gatcttcgag ttcccggatg tgtcaaaaag gatatccagg atggtttctg
     8581 gagctgtccc tcaatttcag aagcttcctg atatccgtct aagaccaggt gattttgaat
     8641 ccctaagtgg tagagaaaag tctcgccata tagggtcagc tcaggggctc ttatactcaa
     8701 tcttagtagc aattcacgac tcaggataca atgatgggac catcttccct gtcaacatat
     8761 acggcaaagt ttcccccaga gactatttga gagggcttgc aagagggatc ttgatagggt
     8821 cctcgatttg cttcttgaca cgaatgacaa atattaacat taacagacct cttgaattga
     8881 tctcaggggt aatttcctat attctcctga ggctggataa tcatccctct ctgtatataa
     8941 tgcttagaga accgtctctt agaggagaaa tattctctat ccctcagaaa atccccgccg
     9001 cttacccaac cactatgaga gaaggcaaca gatcgatttt gtgttacctc caacacgtgc
     9061 tacgctatga gcgagaggca atcacggcgt ccccggagaa tgactggctg tggatcttct
     9121 cagacttcag aagtgtgaaa atgacgtact tgaccctcat tacctaccag tctcacctcc
     9181 tactccagag ggttgagaga aacttgtcta agagtatgag agctactctg cgacaaatga
     9241 gttccttaat gaggcaagtg ctgggtgggc acggagaaga taccttggag tcagacgatg
     9301 acattcaacg attactaaaa gactctttgc gaaagacaag gtgggtggat caagaggtgc
     9361 gccatgcagc tagaaccatg aatggagatt acagccccga caagaaagta tcccacaagg
     9421 caggatgttc agaatgggtc tgctctgctc aacagattgc cgtctccacc tcagccaacc
     9481 cggcccctgt ctcagagctt gacattaggg ccctctctaa gaggtttcaa aaccccttga
     9541 tctcgggcct gagagtggtt cagtgggcaa ccggtgccca ttataagctt aagcctattc
     9601 tagatgatct aaatgttttc ccatctctct gtcttgtaat tggagacggg tcagggggaa
     9661 tatcaagggc agttctcaac atgtttccag attctaagct tgtgttcaac agcctattgg
     9721 aggtgaatga tctgatggct tccggaacac atccactgcc tccttcagca atcatgagtg
     9781 gaggagatga catcatctcc agagtgatag actttgactc aatatgggag aaaccgtccg
     9841 acctgaggaa ctcggccacc tggagatact tccagtcggt tcaaaaacag gtcaacatgt
     9901 cgtatgacct cattatttgt gatgcagaag ttactgatat tgcatctatc aaccggataa
     9961 ctctgttgat gtctgatttc gcattgtcta tagatggacc actttatctg gtcttcaaaa
    10021 cttacgggac tatgctagta aacccggact ataaagctat tcaacatctg tcaagagcgt
    10081 tcccttcggt cacagggttt gtaacccaag taacttcatc cttttcttct gagctatacc
    10141 tccggttctc taaacgagga aagtttttca gggacgccga gtacttgacc tcttccaccc
    10201 ttcgagagat gagccttgtg ttgttcaatt gcagcagccc caaaagtgag atgcagagag
    10261 ctcgttcctt aaactatcaa gacctggtaa ggggatttcc tgaagagatc atatcaaatc
    10321 cttacaacga aatgatcata actctgattg acaatgatgt agagtccttc ctagtccaca
    10381 agatggtgga tgatcttgag ctacagaggg gaactctgtc taaagtggct atcattatat
    10441 ccatcatgat cgttttttcc aatagagtct tcaacatttc caaacctttg actgacccct
    10501 tgttctaccc cccatctgat cctaaaatcc tgaggcactt caacatatgt tgcagtacta
    10561 tgatgtatct atctaccgct ttaggcgacg tccctaactt cgcaagactt catgacctgt
    10621 ataacagacc tataacttgt tacttcagaa aacaagttat tcgagggaat atttatctat
    10681 cttggagttg gtccgatgat accccagtgt tcaagagagt agcctgtaat tctagcttga
    10741 gtctgtcatc tcactggatc aggttgatct acaagatagt gaagactacc agactcattg
    10801 gcagcataaa agacctatca ggagaggtag aacgacatct tcatgggtat aacagatgga
    10861 tcaccctcga ggatatccga tctagatcat ccctactaga ttacagttgt ttgtaagccg
    10921 gatattaccg aaagcctgtg catgctaaaa ttcttgtatg atgcatcttg aaaaaaacaa
    10981 gatcttgaat ccggacctct ggttgtttga ttgttttttc catctttatt gtttttttgt
    11041 taagcgtggg tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga
    11101 ggacgcacgt ccactcggat ggctaaggga gggcggggat ccggctgcta acaaagcccg
    11161 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
    11221 ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg cttggcgtaa tcatggtcat
    11281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    11341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    11401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    11521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    11581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    11641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    12001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    12061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    12121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    12361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacccttg gaggctccag
    12601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    12781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    12901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    12961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    13021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    13081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    13141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    13201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    13261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    13321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
    13441 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    13501 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    13561 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    13621 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    13681 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    13741 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    13801 cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    13861 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
    13921 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    13981 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    14041 gtgagcgcgc ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    14101 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    14161 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    14221 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    14281 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    14341 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    14401 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    14461 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    14521 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    14581 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
    14641 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
    14701 attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtc
//
