LOCUS       sequence-343808-       16242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16242)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-nl.mCherry-FlpO
FEATURES             Location/Qualifiers
     source          1..16242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4032
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3470..3488)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3738..3757
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4048..4068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4072..4092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4096..4116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7671..7682
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9474..9485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12681..12699)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12695..12742
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12770..12786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12770..12786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12783..12805)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12794..12810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12818..12824,12825..12842,12843..12848))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12863..12884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13001..13018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13172..13760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13252..13271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13931..14722,14723..14791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14554..14573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14792..14896)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14923..15379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15010..15029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15220..15241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15505..15527
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15519..15536
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15520..15536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15606..15909
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15910..16113
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16064..16084
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16158..16177
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16158..16176
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggtg agcaagggcg aggaggataa catggccatc
     3361 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3421 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3481 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3541 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3601 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3661 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3721 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3781 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3841 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3901 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3961 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4021 gagctgtaca agagatctcg agctgatcca aaaaagaaga gaaaggtaga tccaaaaaag
     4081 aagagaaagg tagatccaaa aaagaagaga aaggtaggat ccagataact gatcataatc
     4141 agccatacca catttggaat tcgtcgaggg ggcgcgccgc tagacatgaa aaaaactaac
     4201 actcctccgg taccggatcc caccatgagc cagttcgaca tcctgtgcaa gacccccccc
     4261 aaggtgctgg tgcggcagtt cgtggagaga ttcgagaggc ccagcggcga gaagatcgcc
     4321 agctgtgccg ccgagctgac ctacctgtgc tggatggtca cccacaacgg caccgccatc
     4381 aagagggcca ccttcatgag ctacaacacc atcatcagca acagcctgag cttcgacatc
     4441 gtgaacaaga gcctgcagtt caagtacaag acccagaagg ccaccatcct ggaggccagc
     4501 ctgaagaagc tgatccccgc ctgggagttc accatcatcc cttacaacgg ccagaagcac
     4561 cagagcgaca tcaccgacat cgtgtccagc ctgcagctgc agttcgagag cagcgaggag
     4621 gccgacaagg gcaacagcca cagcaagaag atgctgaagg ccctgctgtc cgagggcgag
     4681 agcatctggg agatcaccga gaagatcctg aacagcttcg agtacaccag caggttcacc
     4741 aagaccaaga ccctgtacca gttcctgttc ctggccacat tcatcaactg cggcaggttc
     4801 agcgacatca agaacgtgga ccccaagagc ttcaagctgg tgcagaacaa gtacctgggc
     4861 gtgatcattc agtgcctggt gaccgagacc aagacaagcg tgtccaggca catctacttt
     4921 ttcagcgcca gaggcaggat cgaccccctg gtgtacctgg acgagttcct gaggaacagc
     4981 gagcccgtgc tgaagagagt gaacaggacc ggcaacagca gcagcaacaa gcaggagtac
     5041 cagctgctga aggacaacct ggtgcgcagc tacaacaagg ccctgaagaa gaacgccccc
     5101 taccccatct tcgctatcaa gaacggccct aagagccaca tcggcaggca cctgatgacc
     5161 agctttctga gcatgaaggg cctgaccgag ctgacaaacg tggtgggcaa ctggagcgac
     5221 aagagggcct ccgccgtggc caggaccacc tacacccacc agatcaccgc catccccgac
     5281 cactacttcg ccctggtgtc caggtactac gcctacgacc ccatcagcaa ggagatgatc
     5341 gccctgaagg acgagaccaa ccccatcgag gagtggcagc acatcgagca gctgaagggc
     5401 agcgccgagg gcagcatcag ataccccgcc tggaacggca tcatcagcca ggaggtgctg
     5461 gactacctga gcagctacat caacaggcgg atctgagcta gcggcgccta gccggtcatc
     5521 cttttgacga ttccagtccc gaggataacc tcctctcggg attgggggga atctttggat
     5581 ccagtagtcc tccttgaact ccgtccaaca gggcagattc aagagtcata agactttcat
     5641 taatcatttc agttgatcag acatggtcgt gtagattctc ttaatacggg agatcttcta
     5701 gcagtttcag tgaccaacgg tgctttcatt ctccaggaac tgataccaaa ggttgtggac
     5761 aggccaaggg gtacttcgga tgactctgtg cttgggcaca gaaagaggtc gtagtgtgcc
     5821 ccccgatagc ggactcgaca tgaatcaact aagaaaggca atctgcctcc catgatggac
     5881 ataagcaata gttcacaacc atcttgcatc tcagtgaagt gtacataact attgagggct
     5941 gggtcatcta agcatttcag tcgagaaaaa aactgtagac caaaagaaca actagcaaca
     6001 cttctcatcc agagacccat atcaagatgc tagatccggg agaggtttat gatgacccta
     6061 ttgatccaat tgagtcagag gctgaaccca gaggaacccc cactgtcccc aacatcttga
     6121 ggaactccga ctacaatctc aattctcctt tgatagagga tcctgccaaa ctaatgttag
     6181 aatggttgaa gacagggaac agaccttatc ggatgacttt gacagacaat tgctccaggt
     6241 cttacaaagt tttgaaagat tatttcaaga aagtagattt gggttctctc aaagtgggcg
     6301 gaactgctgc acagtcaatg gtttctctct ggttgtgtgg tgcccactct gaatcaaaca
     6361 ggagccggag atgtataacc gacttggccc atttctattc caagtcatcc cccatagaga
     6421 agctattgaa ttgtacgcta ggaaacagag gcctgagaat cccaccagag ggggtgttaa
     6481 attgcctcga gagggtcaat tatgacaagg catttgggag gtatctggcc aacacgtatt
     6541 cctcttactt gtttttccat gtaatcacct tatacatgaa tgccttagac tgggaagagg
     6601 aaaagaccat cctggcatta tggaaagata taacctcagt ggataccgag aaggacttgg
     6661 tcaaattcaa agatcaaata tggggactgt tgattgtgac aaaggacttt gtttactctc
     6721 agagttcaaa ctgtcttttt gacagaaact acacactgat gctaaaggat cttttcttgt
     6781 ctcgattcaa ctccttaatg attctgcttt ctccccctga gccccgatac tcagatgact
     6841 taatatctca gctgtgccag ctatacatcg ctggggatca agtcttgtcc atgtgtggga
     6901 actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaacagt ttggtccaga
     6961 gggcagagaa gtttaggcct ctcatccacc ccttgggaga ctttcctatg ttcataaaag
     7021 acaaggtgaa tcaacttgaa gggacttttg gtcccagtgc aaagaggttt tttagggttt
     7081 tagatcaatt cgacaacata catgacttag tatttgtgta tggctgttac agacattggg
     7141 ggcaccccta tatagattat cggaagggtc tgtcgaaact atatgatcaa gttcacatta
     7201 agaaagtaat agataagtcc taccaggagt gtttagcaag tgacttggcc agaaggatcc
     7261 tcagatgggg atttgacaag tactccaaat ggtatctaga ttcgagattc cttgcccgag
     7321 accacccctt gactccttat gtcaagaccc aaacatggcc acccaaacat atagtagact
     7381 tggtggggga cacatggcat aagctcccga tcacgcagat ctttgaaatt cctgaatcaa
     7441 tggacccgtc agagatactg gatgacaaat cacattcttt caccagaaca aggttagctt
     7501 cttggctgtc agagaaccga ggggggcctg ttcctagcga gaaggtcatt atcacggccc
     7561 tgtctaagcc acctgtcaat ccccgagagt ttttgaaatc tatagacctc ggaggattgc
     7621 cagatgagga tttgataatt ggcctcaaac caaaggaacg ggagttgaag atcgagggcc
     7681 gattctttgc tctaatgtca tggaatctaa gattatattt tgtcatcacc gaaaagctct
     7741 tggccaacta cattttgcca ctttttgacg cactgactat gacagacaac ctgaacaagg
     7801 tgttcaaaaa gttgatcgac agggtcaccg ggcaagggct tttggactat tctagggtca
     7861 catacgcatt tcacctggac tatgagaaat ggaacaatca tcaaagattg gagtcaacag
     7921 aggatgtatt ctctgtccta gatcaggtgt ttggattgaa gagggtgttt tctagaacac
     7981 acgagttttt tcagaagtcc tggatctatt attcagacag atcagacctc attgggttat
     8041 gggaggacca aatatattgc ttggatatgt ctaacggccc aacctgctgg aatggccaag
     8101 atggcgggct agagggcttg cggcagaagg gctggagtct agtcagttta ttaatgatag
     8161 atagagaatc tcaaaccagg aacacaagaa ccaagatact agctcaagga gacaaccagg
     8221 ttctgtgtcc tacatacatg ttgtcaccgg gattgtctca agaggggctt ctctatgagt
     8281 tagagagcat atcaaggaat gcactctcaa tataccgagc tatcgaggaa ggagcatcta
     8341 agctggggct gatcatcaag aaggaagaga ccatgtgtag ttatgacttt ctcatatatg
     8401 ggaagacccc cttatttcga ggcaacatat tggtgcctga atccaaaaga tgggcccgag
     8461 tctcttgcat ctctaacgac caaatagtca acctcgccaa tataatgtcg acagtatcca
     8521 ccaatgcgct gacagtggca caacactctc aatctctgat caaacctatg agggattttc
     8581 tgctcatgtc agtacaggca gttttccact acctgttgtt tagcccaatc ctaaaaggca
     8641 gagtttataa gattctgagt gctgaagggg agagctttct cctagccatg tcgcggataa
     8701 tctacctaga tccttctttg ggaggggtgt ctggaatgtc tctcgggagg ttccatatac
     8761 gtcagttctc agaccctgtc tctgaagggt tgtcattctg gagagagatc tggttaggct
     8821 ctcatgagtc ctggattcac gcgttgtgtc aggaggccgg gaaccccgat cttggagaga
     8881 gaacactcga gagcttcact cgccttttag aagatcctac taccttaaat atcaaaggag
     8941 gggccagccc taccattcta ctcaaggatg ctatcagaaa ggctctgtac gacgaggtgg
     9001 acaaggtgga aaattcagag tttcgagagg caatcctgct gtccaagacc catagagata
     9061 actttatact ctttttaaaa tctgttgagc ctctgttccc tcgatttctc agtgagctct
     9121 tcagttcgtc tttcttggga ataccggagt caatcattgg actgatacaa aactcccgga
     9181 caataagaag gcagtttaga aagagtctct caagaacttt agaagagtcc ttctacaact
     9241 cagagatcca cgggattaat cggataaccc agacacctca aagggtcgga agggtgtggc
     9301 cttgctcttc agagagggca gatctactta gggagatctc ttgggggagg aaagtggtag
     9361 gcacgacagt tcctcaccct tccgagatgt tggagttgtt tcccaaatcc tccatttcct
     9421 gcacttgtgg agcaacaggg ggaggcaatc ctagagtctc tgtatcagta ctcccgtcct
     9481 tcgatcagtc atttttctca cggggccccc taaagggata cttgggctcg tccacctcca
     9541 tgtcaaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaaaaggg
     9601 ctctatcgtt aaaagaatct ataaactggt tcatcaatag gaattccaat ttggctcaaa
     9661 ctctaattgg aaacatcatg tctctgacag gccctgattt ccctctagaa gaggcccctg
     9721 ttttcaaacg gacagggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag
     9781 ggtattcttc tgtttgccct aaccttctct cccatatctc tgttagtaca gacactatgt
     9841 ctgatttgac ccaaaacggg aagaactatg atttcatgtt tcagccattg atgctttatg
     9901 cgcaaacatg gacatcggaa ctggtacaga gggatacaag acttagagac tccacgtttc
     9961 actggcacct tcggtgcaac agatgtgtga ggcccattga tgatataaca ctggaaactt
    10021 ctcagatctt cgagttcccg gatgtgtcaa aaaggatatc caggatggtt tctggagctg
    10081 tccctcaatt tcagaagctt cctgatatcc gtctaagacc aggtgatttt gaatccctaa
    10141 gtggtagaga aaagtctcgc catatagggt cagctcaggg gctcttatac tcaatcttag
    10201 tagcaattca cgactcagga tacaatgatg ggaccatctt ccctgtcaac atatacggca
    10261 aagtttcccc cagagactat ttgagagggc ttgcaagagg gatcttgata gggtcctcga
    10321 tttgcttctt gacacgaatg acaaatatta acattaacag acctcttgaa ttgatctcag
    10381 gggtaatttc ctatattctc ctgaggctgg ataatcatcc ctctctgtat ataatgctta
    10441 gagaaccgtc tcttagagga gaaatattct ctatccctca gaaaatcccc gccgcttacc
    10501 caaccactat gagagaaggc aacagatcga ttttgtgtta cctccaacac gtgctacgct
    10561 atgagcgaga ggcaatcacg gcgtccccgg agaatgactg gctgtggatc ttctcagact
    10621 tcagaagtgt gaaaatgacg tacttgaccc tcattaccta ccagtctcac ctcctactcc
    10681 agagggttga gagaaacttg tctaagagta tgagagctac tctgcgacaa atgagttcct
    10741 taatgaggca agtgctgggt gggcacggag aagatacctt ggagtcagac gatgacattc
    10801 aacgattact aaaagactct ttgcgaaaga caaggtgggt ggatcaagag gtgcgccatg
    10861 cagctagaac catgaatgga gattacagcc ccgacaagaa agtatcccac aaggcaggat
    10921 gttcagaatg ggtctgctct gctcaacaga ttgccgtctc cacctcagcc aacccggccc
    10981 ctgtctcaga gcttgacatt agggccctct ctaagaggtt tcaaaacccc ttgatctcgg
    11041 gcctgagagt ggttcagtgg gcaaccggtg cccattataa gcttaagcct attctagatg
    11101 atctaaatgt tttcccatct ctctgtcttg taattggaga cgggtcaggg ggaatatcaa
    11161 gggcagttct caacatgttt ccagattcta agcttgtgtt caacagccta ttggaggtga
    11221 atgatctgat ggcttccgga acacatccac tgcctccttc agcaatcatg agtggaggag
    11281 atgacatcat ctccagagtg atagactttg actcaatatg ggagaaaccg tccgacctga
    11341 ggaactcggc cacctggaga tacttccagt cggttcaaaa acaggtcaac atgtcgtatg
    11401 acctcattat ttgtgatgca gaagttactg atattgcatc tatcaaccgg ataactctgt
    11461 tgatgtctga tttcgcattg tctatagatg gaccacttta tctggtcttc aaaacttacg
    11521 ggactatgct agtaaacccg gactataaag ctattcaaca tctgtcaaga gcgttccctt
    11581 cggtcacagg gtttgtaacc caagtaactt catccttttc ttctgagcta tacctccggt
    11641 tctctaaacg aggaaagttt ttcagggacg ccgagtactt gacctcttcc acccttcgag
    11701 agatgagcct tgtgttgttc aattgcagca gccccaaaag tgagatgcag agagctcgtt
    11761 ccttaaacta tcaagacctg gtaaggggat ttcctgaaga gatcatatca aatccttaca
    11821 acgaaatgat cataactctg attgacaatg atgtagagtc cttcctagtc cacaagatgg
    11881 tggatgatct tgagctacag aggggaactc tgtctaaagt ggctatcatt atatccatca
    11941 tgatcgtttt ttccaataga gtcttcaaca tttccaaacc tttgactgac cccttgttct
    12001 accccccatc tgatcctaaa atcctgaggc acttcaacat atgttgcagt actatgatgt
    12061 atctatctac cgctttaggc gacgtcccta acttcgcaag acttcatgac ctgtataaca
    12121 gacctataac ttgttacttc agaaaacaag ttattcgagg gaatatttat ctatcttgga
    12181 gttggtccga tgatacccca gtgttcaaga gagtagcctg taattctagc ttgagtctgt
    12241 catctcactg gatcaggttg atctacaaga tagtgaagac taccagactc attggcagca
    12301 taaaagacct atcaggagag gtagaacgac atcttcatgg gtataacaga tggatcaccc
    12361 tcgaggatat ccgatctaga tcatccctac tagattacag ttgtttgtaa gccggatatt
    12421 accgaaagcc tgtgcatgct aaaattcttg tatgatgcat cttgaaaaaa acaagatctt
    12481 gaatccggac ctctggttgt ttgattgttt tttccatctt tattgttttt ttgttaagcg
    12541 tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc
    12601 acgtccactc ggatggctaa gggagggcgg ggatccggct gctaacaaag cccgaaagga
    12661 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
    12721 acgggtcttg aggggttttt tgctgaaagt cgcgcttggc gtaatcatgg tcatagctgt
    12781 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    12841 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    12901 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    13021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    13081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    13141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    13201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    13261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    13321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    13381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    13441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    13501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    13561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    13621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    13681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    13741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    13801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    13861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    13921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    13981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    14041 catctggccc cagtgctgca atgataccgc gagacccacc cttggaggct ccagatttat
    14101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    14161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    14221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    14281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    14341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    14401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    14461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    14521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    14581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    14641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    14701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    14761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    14821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    14881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
    14941 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    15001 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    15061 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    15121 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    15181 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    15241 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    15301 cggcctattg gttaaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    15361 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    15421 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    15481 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    15541 gcgcctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    15601 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    15661 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    15721 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    15781 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    15841 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    15901 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    15961 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    16021 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    16081 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
    16141 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagattaag
    16201 cgtctgatga gtccgtgagg acgaaacccg gcgtaccggg tc
//
