LOCUS       sequence-343809-       16248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-nl.EGFP-FlpO
FEATURES             Location/Qualifiers
     source          1..16248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3370..3391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3631..3650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3978..3999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4054..4074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4078..4098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4102..4122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7677..7688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9480..9491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12687..12705)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12701..12748
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12776..12792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12776..12792)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12789..12811)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12800..12816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12824..12830,12831..12848,12849..12854))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12869..12890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13007..13024)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13178..13766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13258..13277)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13937..14728,14729..14797))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14560..14579
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14798..14902)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14929..15385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15016..15035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15226..15247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15511..15533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15525..15542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15526..15542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15612..15915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15916..16119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16070..16090
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16164..16183
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16164..16182
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa ggctcgagct gatccaaaaa agaagagaaa ggtagatcca
     4081 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccag ataactgatc
     4141 ataatcagcc ataccacatt tggaattcgt cgagggggcg cgccgctaga catgaaaaaa
     4201 actaacactc ctccggtacc ggatcccacc atgagccagt tcgacatcct gtgcaagacc
     4261 ccccccaagg tgctggtgcg gcagttcgtg gagagattcg agaggcccag cggcgagaag
     4321 atcgccagct gtgccgccga gctgacctac ctgtgctgga tggtcaccca caacggcacc
     4381 gccatcaaga gggccacctt catgagctac aacaccatca tcagcaacag cctgagcttc
     4441 gacatcgtga acaagagcct gcagttcaag tacaagaccc agaaggccac catcctggag
     4501 gccagcctga agaagctgat ccccgcctgg gagttcacca tcatccctta caacggccag
     4561 aagcaccaga gcgacatcac cgacatcgtg tccagcctgc agctgcagtt cgagagcagc
     4621 gaggaggccg acaagggcaa cagccacagc aagaagatgc tgaaggccct gctgtccgag
     4681 ggcgagagca tctgggagat caccgagaag atcctgaaca gcttcgagta caccagcagg
     4741 ttcaccaaga ccaagaccct gtaccagttc ctgttcctgg ccacattcat caactgcggc
     4801 aggttcagcg acatcaagaa cgtggacccc aagagcttca agctggtgca gaacaagtac
     4861 ctgggcgtga tcattcagtg cctggtgacc gagaccaaga caagcgtgtc caggcacatc
     4921 tactttttca gcgccagagg caggatcgac cccctggtgt acctggacga gttcctgagg
     4981 aacagcgagc ccgtgctgaa gagagtgaac aggaccggca acagcagcag caacaagcag
     5041 gagtaccagc tgctgaagga caacctggtg cgcagctaca acaaggccct gaagaagaac
     5101 gccccctacc ccatcttcgc tatcaagaac ggccctaaga gccacatcgg caggcacctg
     5161 atgaccagct ttctgagcat gaagggcctg accgagctga caaacgtggt gggcaactgg
     5221 agcgacaaga gggcctccgc cgtggccagg accacctaca cccaccagat caccgccatc
     5281 cccgaccact acttcgccct ggtgtccagg tactacgcct acgaccccat cagcaaggag
     5341 atgatcgccc tgaaggacga gaccaacccc atcgaggagt ggcagcacat cgagcagctg
     5401 aagggcagcg ccgagggcag catcagatac cccgcctgga acggcatcat cagccaggag
     5461 gtgctggact acctgagcag ctacatcaac aggcggatct gagctagcgg cgcctagccg
     5521 gtcatccttt tgacgattcc agtcccgagg ataacctcct ctcgggattg gggggaatct
     5581 ttggatccag tagtcctcct tgaactccgt ccaacagggc agattcaaga gtcataagac
     5641 tttcattaat catttcagtt gatcagacat ggtcgtgtag attctcttaa tacgggagat
     5701 cttctagcag tttcagtgac caacggtgct ttcattctcc aggaactgat accaaaggtt
     5761 gtggacaggc caaggggtac ttcggatgac tctgtgcttg ggcacagaaa gaggtcgtag
     5821 tgtgcccccc gatagcggac tcgacatgaa tcaactaaga aaggcaatct gcctcccatg
     5881 atggacataa gcaatagttc acaaccatct tgcatctcag tgaagtgtac ataactattg
     5941 agggctgggt catctaagca tttcagtcga gaaaaaaact gtagaccaaa agaacaacta
     6001 gcaacacttc tcatccagag acccatatca agatgctaga tccgggagag gtttatgatg
     6061 accctattga tccaattgag tcagaggctg aacccagagg aacccccact gtccccaaca
     6121 tcttgaggaa ctccgactac aatctcaatt ctcctttgat agaggatcct gccaaactaa
     6181 tgttagaatg gttgaagaca gggaacagac cttatcggat gactttgaca gacaattgct
     6241 ccaggtctta caaagttttg aaagattatt tcaagaaagt agatttgggt tctctcaaag
     6301 tgggcggaac tgctgcacag tcaatggttt ctctctggtt gtgtggtgcc cactctgaat
     6361 caaacaggag ccggagatgt ataaccgact tggcccattt ctattccaag tcatccccca
     6421 tagagaagct attgaattgt acgctaggaa acagaggcct gagaatccca ccagaggggg
     6481 tgttaaattg cctcgagagg gtcaattatg acaaggcatt tgggaggtat ctggccaaca
     6541 cgtattcctc ttacttgttt ttccatgtaa tcaccttata catgaatgcc ttagactggg
     6601 aagaggaaaa gaccatcctg gcattatgga aagatataac ctcagtggat accgagaagg
     6661 acttggtcaa attcaaagat caaatatggg gactgttgat tgtgacaaag gactttgttt
     6721 actctcagag ttcaaactgt ctttttgaca gaaactacac actgatgcta aaggatcttt
     6781 tcttgtctcg attcaactcc ttaatgattc tgctttctcc ccctgagccc cgatactcag
     6841 atgacttaat atctcagctg tgccagctat acatcgctgg ggatcaagtc ttgtccatgt
     6901 gtgggaactc cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aacagtttgg
     6961 tccagagggc agagaagttt aggcctctca tccacccctt gggagacttt cctatgttca
     7021 taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc cagtgcaaag aggtttttta
     7081 gggttttaga tcaattcgac aacatacatg acttagtatt tgtgtatggc tgttacagac
     7141 attgggggca cccctatata gattatcgga agggtctgtc gaaactatat gatcaagttc
     7201 acattaagaa agtaatagat aagtcctacc aggagtgttt agcaagtgac ttggccagaa
     7261 ggatcctcag atggggattt gacaagtact ccaaatggta tctagattcg agattccttg
     7321 cccgagacca ccccttgact ccttatgtca agacccaaac atggccaccc aaacatatag
     7381 tagacttggt gggggacaca tggcataagc tcccgatcac gcagatcttt gaaattcctg
     7441 aatcaatgga cccgtcagag atactggatg acaaatcaca ttctttcacc agaacaaggt
     7501 tagcttcttg gctgtcagag aaccgagggg ggcctgttcc tagcgagaag gtcattatca
     7561 cggccctgtc taagccacct gtcaatcccc gagagttttt gaaatctata gacctcggag
     7621 gattgccaga tgaggatttg ataattggcc tcaaaccaaa ggaacgggag ttgaagatcg
     7681 agggccgatt ctttgctcta atgtcatgga atctaagatt atattttgtc atcaccgaaa
     7741 agctcttggc caactacatt ttgccacttt ttgacgcact gactatgaca gacaacctga
     7801 acaaggtgtt caaaaagttg atcgacaggg tcaccgggca agggcttttg gactattcta
     7861 gggtcacata cgcatttcac ctggactatg agaaatggaa caatcatcaa agattggagt
     7921 caacagagga tgtattctct gtcctagatc aggtgtttgg attgaagagg gtgttttcta
     7981 gaacacacga gttttttcag aagtcctgga tctattattc agacagatca gacctcattg
     8041 ggttatggga ggaccaaata tattgcttgg atatgtctaa cggcccaacc tgctggaatg
     8101 gccaagatgg cgggctagag ggcttgcggc agaagggctg gagtctagtc agtttattaa
     8161 tgatagatag agaatctcaa accaggaaca caagaaccaa gatactagct caaggagaca
     8221 accaggttct gtgtcctaca tacatgttgt caccgggatt gtctcaagag gggcttctct
     8281 atgagttaga gagcatatca aggaatgcac tctcaatata ccgagctatc gaggaaggag
     8341 catctaagct ggggctgatc atcaagaagg aagagaccat gtgtagttat gactttctca
     8401 tatatgggaa gaccccctta tttcgaggca acatattggt gcctgaatcc aaaagatggg
     8461 cccgagtctc ttgcatctct aacgaccaaa tagtcaacct cgccaatata atgtcgacag
     8521 tatccaccaa tgcgctgaca gtggcacaac actctcaatc tctgatcaaa cctatgaggg
     8581 attttctgct catgtcagta caggcagttt tccactacct gttgtttagc ccaatcctaa
     8641 aaggcagagt ttataagatt ctgagtgctg aaggggagag ctttctccta gccatgtcgc
     8701 ggataatcta cctagatcct tctttgggag gggtgtctgg aatgtctctc gggaggttcc
     8761 atatacgtca gttctcagac cctgtctctg aagggttgtc attctggaga gagatctggt
     8821 taggctctca tgagtcctgg attcacgcgt tgtgtcagga ggccgggaac cccgatcttg
     8881 gagagagaac actcgagagc ttcactcgcc ttttagaaga tcctactacc ttaaatatca
     8941 aaggaggggc cagccctacc attctactca aggatgctat cagaaaggct ctgtacgacg
     9001 aggtggacaa ggtggaaaat tcagagtttc gagaggcaat cctgctgtcc aagacccata
     9061 gagataactt tatactcttt ttaaaatctg ttgagcctct gttccctcga tttctcagtg
     9121 agctcttcag ttcgtctttc ttgggaatac cggagtcaat cattggactg atacaaaact
     9181 cccggacaat aagaaggcag tttagaaaga gtctctcaag aactttagaa gagtccttct
     9241 acaactcaga gatccacggg attaatcgga taacccagac acctcaaagg gtcggaaggg
     9301 tgtggccttg ctcttcagag agggcagatc tacttaggga gatctcttgg gggaggaaag
     9361 tggtaggcac gacagttcct cacccttccg agatgttgga gttgtttccc aaatcctcca
     9421 tttcctgcac ttgtggagca acagggggag gcaatcctag agtctctgta tcagtactcc
     9481 cgtccttcga tcagtcattt ttctcacggg gccccctaaa gggatacttg ggctcgtcca
     9541 cctccatgtc aacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga
     9601 aaagggctct atcgttaaaa gaatctataa actggttcat caataggaat tccaatttgg
     9661 ctcaaactct aattggaaac atcatgtctc tgacaggccc tgatttccct ctagaagagg
     9721 cccctgtttt caaacggaca gggtcagcct tgcataggtt caagtctgcc agatacagcg
     9781 aaggagggta ttcttctgtt tgccctaacc ttctctccca tatctctgtt agtacagaca
     9841 ctatgtctga tttgacccaa aacgggaaga actatgattt catgtttcag ccattgatgc
     9901 tttatgcgca aacatggaca tcggaactgg tacagaggga tacaagactt agagactcca
     9961 cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc cattgatgat ataacactgg
    10021 aaacttctca gatcttcgag ttcccggatg tgtcaaaaag gatatccagg atggtttctg
    10081 gagctgtccc tcaatttcag aagcttcctg atatccgtct aagaccaggt gattttgaat
    10141 ccctaagtgg tagagaaaag tctcgccata tagggtcagc tcaggggctc ttatactcaa
    10201 tcttagtagc aattcacgac tcaggataca atgatgggac catcttccct gtcaacatat
    10261 acggcaaagt ttcccccaga gactatttga gagggcttgc aagagggatc ttgatagggt
    10321 cctcgatttg cttcttgaca cgaatgacaa atattaacat taacagacct cttgaattga
    10381 tctcaggggt aatttcctat attctcctga ggctggataa tcatccctct ctgtatataa
    10441 tgcttagaga accgtctctt agaggagaaa tattctctat ccctcagaaa atccccgccg
    10501 cttacccaac cactatgaga gaaggcaaca gatcgatttt gtgttacctc caacacgtgc
    10561 tacgctatga gcgagaggca atcacggcgt ccccggagaa tgactggctg tggatcttct
    10621 cagacttcag aagtgtgaaa atgacgtact tgaccctcat tacctaccag tctcacctcc
    10681 tactccagag ggttgagaga aacttgtcta agagtatgag agctactctg cgacaaatga
    10741 gttccttaat gaggcaagtg ctgggtgggc acggagaaga taccttggag tcagacgatg
    10801 acattcaacg attactaaaa gactctttgc gaaagacaag gtgggtggat caagaggtgc
    10861 gccatgcagc tagaaccatg aatggagatt acagccccga caagaaagta tcccacaagg
    10921 caggatgttc agaatgggtc tgctctgctc aacagattgc cgtctccacc tcagccaacc
    10981 cggcccctgt ctcagagctt gacattaggg ccctctctaa gaggtttcaa aaccccttga
    11041 tctcgggcct gagagtggtt cagtgggcaa ccggtgccca ttataagctt aagcctattc
    11101 tagatgatct aaatgttttc ccatctctct gtcttgtaat tggagacggg tcagggggaa
    11161 tatcaagggc agttctcaac atgtttccag attctaagct tgtgttcaac agcctattgg
    11221 aggtgaatga tctgatggct tccggaacac atccactgcc tccttcagca atcatgagtg
    11281 gaggagatga catcatctcc agagtgatag actttgactc aatatgggag aaaccgtccg
    11341 acctgaggaa ctcggccacc tggagatact tccagtcggt tcaaaaacag gtcaacatgt
    11401 cgtatgacct cattatttgt gatgcagaag ttactgatat tgcatctatc aaccggataa
    11461 ctctgttgat gtctgatttc gcattgtcta tagatggacc actttatctg gtcttcaaaa
    11521 cttacgggac tatgctagta aacccggact ataaagctat tcaacatctg tcaagagcgt
    11581 tcccttcggt cacagggttt gtaacccaag taacttcatc cttttcttct gagctatacc
    11641 tccggttctc taaacgagga aagtttttca gggacgccga gtacttgacc tcttccaccc
    11701 ttcgagagat gagccttgtg ttgttcaatt gcagcagccc caaaagtgag atgcagagag
    11761 ctcgttcctt aaactatcaa gacctggtaa ggggatttcc tgaagagatc atatcaaatc
    11821 cttacaacga aatgatcata actctgattg acaatgatgt agagtccttc ctagtccaca
    11881 agatggtgga tgatcttgag ctacagaggg gaactctgtc taaagtggct atcattatat
    11941 ccatcatgat cgttttttcc aatagagtct tcaacatttc caaacctttg actgacccct
    12001 tgttctaccc cccatctgat cctaaaatcc tgaggcactt caacatatgt tgcagtacta
    12061 tgatgtatct atctaccgct ttaggcgacg tccctaactt cgcaagactt catgacctgt
    12121 ataacagacc tataacttgt tacttcagaa aacaagttat tcgagggaat atttatctat
    12181 cttggagttg gtccgatgat accccagtgt tcaagagagt agcctgtaat tctagcttga
    12241 gtctgtcatc tcactggatc aggttgatct acaagatagt gaagactacc agactcattg
    12301 gcagcataaa agacctatca ggagaggtag aacgacatct tcatgggtat aacagatgga
    12361 tcaccctcga ggatatccga tctagatcat ccctactaga ttacagttgt ttgtaagccg
    12421 gatattaccg aaagcctgtg catgctaaaa ttcttgtatg atgcatcttg aaaaaaacaa
    12481 gatcttgaat ccggacctct ggttgtttga ttgttttttc catctttatt gtttttttgt
    12541 taagcgtggg tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga
    12601 ggacgcacgt ccactcggat ggctaaggga gggcggggat ccggctgcta acaaagcccg
    12661 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
    12721 ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg cttggcgtaa tcatggtcat
    12781 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    12841 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    12901 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    12961 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    13021 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    13081 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    13141 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    13201 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13261 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13321 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13381 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13441 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13501 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13561 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    13621 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13681 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13741 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    13801 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    13861 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    13921 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    13981 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    14041 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacccttg gaggctccag
    14101 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    14161 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    14221 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    14281 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14341 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14401 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14461 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14521 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    14581 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    14641 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    14701 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    14761 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    14821 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    14881 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
    14941 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    15001 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    15061 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    15121 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    15181 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    15241 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    15301 cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    15361 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
    15421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    15481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    15541 gtgagcgcgc ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    15601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    15661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    15721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    15781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    15841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    15901 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    15961 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    16021 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    16081 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
    16141 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
    16201 attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtc
//
