LOCUS       sequence-343811-       16628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16628)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-nl.EGFP-SypGFP
FEATURES             Location/Qualifiers
     source          1..16628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4054..4074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4078..4098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4102..4122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4221..4230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5157..5166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5163..5165,5166..5168,5169..5882)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5163..5165,5166..5168,5169..5879)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8057..8068
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9860..9871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13067..13085)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13081..13128
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13156..13172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13156..13172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13169..13191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13180..13196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13204..13210,13211..13228,13229..13234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13249..13270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13387..13404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13558..14146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13638..13657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14317..15108,15109..15177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14940..14959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15178..15282)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15309..15765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15396..15415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15606..15627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15891..15913
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15905..15922
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15906..15922
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15992..16295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16296..16499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16450..16470
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16544..16563
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16544..16562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa ggctcgagct gatccaaaaa agaagagaaa ggtagatcca
     4081 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccag ataactgatc
     4141 ataatcagcc ataccacatt tggaattcgt cgagggggcg cgccgctaga catgaaaaaa
     4201 actaacactc ctccggtacc gccaccatgg acgtggtgaa tcagctggtg gctgggggtc
     4261 agttccgggt ggtcaaggag ccccttggct tcgtgaaggt gctgcagtgg gtctttgcca
     4321 tcttcgcctt tgctacgtgt ggcagctaca ccggggagct tcggctgagc gtggagtgtg
     4381 ccaacaagac ggagagtgcc ctcaacatcg aagttgaatt cgagtacccc ttcaggctgc
     4441 accaagtgta ctttgatgca ccctcctgcg tcaaaggggg cactaccaag atcttcctgg
     4501 ttggggacta ctcctcgtcg gctgaattct ttgtcaccgt ggctgtgttt gccttcctct
     4561 actccatggg ggccctggcc acctacatct tcctgcagaa caagtaccga gagaacaaca
     4621 aagggcctat gatggacttt ctggctacag ccgtgttcgc tttcatgtgg ctagttagtt
     4681 catcagcctg ggccaaaggc ctgtccgatg tgaagatggc cacggaccca gagaacatta
     4741 tcaaggagat gcccatgtgc cgccagacag ggaacacatg caaggaactg agggaccctg
     4801 tgacttcagg actcaacacc tcagtggtgt ttggcttcct gaacctggtg ctctgggttg
     4861 gcaacttatg gttcgtgttc aaggagacag gctgggcagc cccattcatg cgcgcacctc
     4921 caggcgcccc ggaaaagcaa ccagcacctg gcgatgccta cggcgatgcg ggctacgggc
     4981 agggccccgg aggctatggg ccccaggact cctacgggcc tcagggtggt tatcaacccg
     5041 attacgggca gccagccagc ggtggcggtg gctacgggcc tcagggcgac tatgggcagc
     5101 aaggctatgg ccaacagggt gcgcccacct ccttctccaa tcagatggga tccatcgcca
     5161 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5221 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5281 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5341 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5401 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5581 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5641 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5701 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5761 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5821 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5881 aagctagcgg cgcctagccg gtcatccttt tgacgattcc agtcccgagg ataacctcct
     5941 ctcgggattg gggggaatct ttggatccag tagtcctcct tgaactccgt ccaacagggc
     6001 agattcaaga gtcataagac tttcattaat catttcagtt gatcagacat ggtcgtgtag
     6061 attctcttaa tacgggagat cttctagcag tttcagtgac caacggtgct ttcattctcc
     6121 aggaactgat accaaaggtt gtggacaggc caaggggtac ttcggatgac tctgtgcttg
     6181 ggcacagaaa gaggtcgtag tgtgcccccc gatagcggac tcgacatgaa tcaactaaga
     6241 aaggcaatct gcctcccatg atggacataa gcaatagttc acaaccatct tgcatctcag
     6301 tgaagtgtac ataactattg agggctgggt catctaagca tttcagtcga gaaaaaaact
     6361 gtagaccaaa agaacaacta gcaacacttc tcatccagag acccatatca agatgctaga
     6421 tccgggagag gtttatgatg accctattga tccaattgag tcagaggctg aacccagagg
     6481 aacccccact gtccccaaca tcttgaggaa ctccgactac aatctcaatt ctcctttgat
     6541 agaggatcct gccaaactaa tgttagaatg gttgaagaca gggaacagac cttatcggat
     6601 gactttgaca gacaattgct ccaggtctta caaagttttg aaagattatt tcaagaaagt
     6661 agatttgggt tctctcaaag tgggcggaac tgctgcacag tcaatggttt ctctctggtt
     6721 gtgtggtgcc cactctgaat caaacaggag ccggagatgt ataaccgact tggcccattt
     6781 ctattccaag tcatccccca tagagaagct attgaattgt acgctaggaa acagaggcct
     6841 gagaatccca ccagaggggg tgttaaattg cctcgagagg gtcaattatg acaaggcatt
     6901 tgggaggtat ctggccaaca cgtattcctc ttacttgttt ttccatgtaa tcaccttata
     6961 catgaatgcc ttagactggg aagaggaaaa gaccatcctg gcattatgga aagatataac
     7021 ctcagtggat accgagaagg acttggtcaa attcaaagat caaatatggg gactgttgat
     7081 tgtgacaaag gactttgttt actctcagag ttcaaactgt ctttttgaca gaaactacac
     7141 actgatgcta aaggatcttt tcttgtctcg attcaactcc ttaatgattc tgctttctcc
     7201 ccctgagccc cgatactcag atgacttaat atctcagctg tgccagctat acatcgctgg
     7261 ggatcaagtc ttgtccatgt gtgggaactc cggctatgaa gtcatcaaaa tattggagcc
     7321 atatgtcgtg aacagtttgg tccagagggc agagaagttt aggcctctca tccacccctt
     7381 gggagacttt cctatgttca taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc
     7441 cagtgcaaag aggtttttta gggttttaga tcaattcgac aacatacatg acttagtatt
     7501 tgtgtatggc tgttacagac attgggggca cccctatata gattatcgga agggtctgtc
     7561 gaaactatat gatcaagttc acattaagaa agtaatagat aagtcctacc aggagtgttt
     7621 agcaagtgac ttggccagaa ggatcctcag atggggattt gacaagtact ccaaatggta
     7681 tctagattcg agattccttg cccgagacca ccccttgact ccttatgtca agacccaaac
     7741 atggccaccc aaacatatag tagacttggt gggggacaca tggcataagc tcccgatcac
     7801 gcagatcttt gaaattcctg aatcaatgga cccgtcagag atactggatg acaaatcaca
     7861 ttctttcacc agaacaaggt tagcttcttg gctgtcagag aaccgagggg ggcctgttcc
     7921 tagcgagaag gtcattatca cggccctgtc taagccacct gtcaatcccc gagagttttt
     7981 gaaatctata gacctcggag gattgccaga tgaggatttg ataattggcc tcaaaccaaa
     8041 ggaacgggag ttgaagatcg agggccgatt ctttgctcta atgtcatgga atctaagatt
     8101 atattttgtc atcaccgaaa agctcttggc caactacatt ttgccacttt ttgacgcact
     8161 gactatgaca gacaacctga acaaggtgtt caaaaagttg atcgacaggg tcaccgggca
     8221 agggcttttg gactattcta gggtcacata cgcatttcac ctggactatg agaaatggaa
     8281 caatcatcaa agattggagt caacagagga tgtattctct gtcctagatc aggtgtttgg
     8341 attgaagagg gtgttttcta gaacacacga gttttttcag aagtcctgga tctattattc
     8401 agacagatca gacctcattg ggttatggga ggaccaaata tattgcttgg atatgtctaa
     8461 cggcccaacc tgctggaatg gccaagatgg cgggctagag ggcttgcggc agaagggctg
     8521 gagtctagtc agtttattaa tgatagatag agaatctcaa accaggaaca caagaaccaa
     8581 gatactagct caaggagaca accaggttct gtgtcctaca tacatgttgt caccgggatt
     8641 gtctcaagag gggcttctct atgagttaga gagcatatca aggaatgcac tctcaatata
     8701 ccgagctatc gaggaaggag catctaagct ggggctgatc atcaagaagg aagagaccat
     8761 gtgtagttat gactttctca tatatgggaa gaccccctta tttcgaggca acatattggt
     8821 gcctgaatcc aaaagatggg cccgagtctc ttgcatctct aacgaccaaa tagtcaacct
     8881 cgccaatata atgtcgacag tatccaccaa tgcgctgaca gtggcacaac actctcaatc
     8941 tctgatcaaa cctatgaggg attttctgct catgtcagta caggcagttt tccactacct
     9001 gttgtttagc ccaatcctaa aaggcagagt ttataagatt ctgagtgctg aaggggagag
     9061 ctttctccta gccatgtcgc ggataatcta cctagatcct tctttgggag gggtgtctgg
     9121 aatgtctctc gggaggttcc atatacgtca gttctcagac cctgtctctg aagggttgtc
     9181 attctggaga gagatctggt taggctctca tgagtcctgg attcacgcgt tgtgtcagga
     9241 ggccgggaac cccgatcttg gagagagaac actcgagagc ttcactcgcc ttttagaaga
     9301 tcctactacc ttaaatatca aaggaggggc cagccctacc attctactca aggatgctat
     9361 cagaaaggct ctgtacgacg aggtggacaa ggtggaaaat tcagagtttc gagaggcaat
     9421 cctgctgtcc aagacccata gagataactt tatactcttt ttaaaatctg ttgagcctct
     9481 gttccctcga tttctcagtg agctcttcag ttcgtctttc ttgggaatac cggagtcaat
     9541 cattggactg atacaaaact cccggacaat aagaaggcag tttagaaaga gtctctcaag
     9601 aactttagaa gagtccttct acaactcaga gatccacggg attaatcgga taacccagac
     9661 acctcaaagg gtcggaaggg tgtggccttg ctcttcagag agggcagatc tacttaggga
     9721 gatctcttgg gggaggaaag tggtaggcac gacagttcct cacccttccg agatgttgga
     9781 gttgtttccc aaatcctcca tttcctgcac ttgtggagca acagggggag gcaatcctag
     9841 agtctctgta tcagtactcc cgtccttcga tcagtcattt ttctcacggg gccccctaaa
     9901 gggatacttg ggctcgtcca cctccatgtc aacccagcta ttccatgcat gggaaaaagt
     9961 cactaatgtt catgtggtga aaagggctct atcgttaaaa gaatctataa actggttcat
    10021 caataggaat tccaatttgg ctcaaactct aattggaaac atcatgtctc tgacaggccc
    10081 tgatttccct ctagaagagg cccctgtttt caaacggaca gggtcagcct tgcataggtt
    10141 caagtctgcc agatacagcg aaggagggta ttcttctgtt tgccctaacc ttctctccca
    10201 tatctctgtt agtacagaca ctatgtctga tttgacccaa aacgggaaga actatgattt
    10261 catgtttcag ccattgatgc tttatgcgca aacatggaca tcggaactgg tacagaggga
    10321 tacaagactt agagactcca cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc
    10381 cattgatgat ataacactgg aaacttctca gatcttcgag ttcccggatg tgtcaaaaag
    10441 gatatccagg atggtttctg gagctgtccc tcaatttcag aagcttcctg atatccgtct
    10501 aagaccaggt gattttgaat ccctaagtgg tagagaaaag tctcgccata tagggtcagc
    10561 tcaggggctc ttatactcaa tcttagtagc aattcacgac tcaggataca atgatgggac
    10621 catcttccct gtcaacatat acggcaaagt ttcccccaga gactatttga gagggcttgc
    10681 aagagggatc ttgatagggt cctcgatttg cttcttgaca cgaatgacaa atattaacat
    10741 taacagacct cttgaattga tctcaggggt aatttcctat attctcctga ggctggataa
    10801 tcatccctct ctgtatataa tgcttagaga accgtctctt agaggagaaa tattctctat
    10861 ccctcagaaa atccccgccg cttacccaac cactatgaga gaaggcaaca gatcgatttt
    10921 gtgttacctc caacacgtgc tacgctatga gcgagaggca atcacggcgt ccccggagaa
    10981 tgactggctg tggatcttct cagacttcag aagtgtgaaa atgacgtact tgaccctcat
    11041 tacctaccag tctcacctcc tactccagag ggttgagaga aacttgtcta agagtatgag
    11101 agctactctg cgacaaatga gttccttaat gaggcaagtg ctgggtgggc acggagaaga
    11161 taccttggag tcagacgatg acattcaacg attactaaaa gactctttgc gaaagacaag
    11221 gtgggtggat caagaggtgc gccatgcagc tagaaccatg aatggagatt acagccccga
    11281 caagaaagta tcccacaagg caggatgttc agaatgggtc tgctctgctc aacagattgc
    11341 cgtctccacc tcagccaacc cggcccctgt ctcagagctt gacattaggg ccctctctaa
    11401 gaggtttcaa aaccccttga tctcgggcct gagagtggtt cagtgggcaa ccggtgccca
    11461 ttataagctt aagcctattc tagatgatct aaatgttttc ccatctctct gtcttgtaat
    11521 tggagacggg tcagggggaa tatcaagggc agttctcaac atgtttccag attctaagct
    11581 tgtgttcaac agcctattgg aggtgaatga tctgatggct tccggaacac atccactgcc
    11641 tccttcagca atcatgagtg gaggagatga catcatctcc agagtgatag actttgactc
    11701 aatatgggag aaaccgtccg acctgaggaa ctcggccacc tggagatact tccagtcggt
    11761 tcaaaaacag gtcaacatgt cgtatgacct cattatttgt gatgcagaag ttactgatat
    11821 tgcatctatc aaccggataa ctctgttgat gtctgatttc gcattgtcta tagatggacc
    11881 actttatctg gtcttcaaaa cttacgggac tatgctagta aacccggact ataaagctat
    11941 tcaacatctg tcaagagcgt tcccttcggt cacagggttt gtaacccaag taacttcatc
    12001 cttttcttct gagctatacc tccggttctc taaacgagga aagtttttca gggacgccga
    12061 gtacttgacc tcttccaccc ttcgagagat gagccttgtg ttgttcaatt gcagcagccc
    12121 caaaagtgag atgcagagag ctcgttcctt aaactatcaa gacctggtaa ggggatttcc
    12181 tgaagagatc atatcaaatc cttacaacga aatgatcata actctgattg acaatgatgt
    12241 agagtccttc ctagtccaca agatggtgga tgatcttgag ctacagaggg gaactctgtc
    12301 taaagtggct atcattatat ccatcatgat cgttttttcc aatagagtct tcaacatttc
    12361 caaacctttg actgacccct tgttctaccc cccatctgat cctaaaatcc tgaggcactt
    12421 caacatatgt tgcagtacta tgatgtatct atctaccgct ttaggcgacg tccctaactt
    12481 cgcaagactt catgacctgt ataacagacc tataacttgt tacttcagaa aacaagttat
    12541 tcgagggaat atttatctat cttggagttg gtccgatgat accccagtgt tcaagagagt
    12601 agcctgtaat tctagcttga gtctgtcatc tcactggatc aggttgatct acaagatagt
    12661 gaagactacc agactcattg gcagcataaa agacctatca ggagaggtag aacgacatct
    12721 tcatgggtat aacagatgga tcaccctcga ggatatccga tctagatcat ccctactaga
    12781 ttacagttgt ttgtaagccg gatattaccg aaagcctgtg catgctaaaa ttcttgtatg
    12841 atgcatcttg aaaaaaacaa gatcttgaat ccggacctct ggttgtttga ttgttttttc
    12901 catctttatt gtttttttgt taagcgtggg tcggcatggc atctccacct cctcgcggtc
    12961 cgacctgggc atccgaagga ggacgcacgt ccactcggat ggctaaggga gggcggggat
    13021 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
    13081 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg
    13141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    13201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    13261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    13321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    13381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    13441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    13501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    13561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    13621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    13681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    13741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    13801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    13861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    13921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    13981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    14041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    14101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    14161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    14221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    14281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    14341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    14401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    14461 cccacccttg gaggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    14521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    14581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    14641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    14701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    14761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    14821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    14881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    14941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    15001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    15061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    15121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    15181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    15241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    15301 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    15361 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    15421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    15481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    15541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    15601 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    15661 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaaatgag ctgatttaac
    15721 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
    15781 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    15841 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    15901 acgttgtaaa acgacggcca gtgagcgcgc ctagttatta atagtaatca attacggggt
    15961 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    16021 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    16081 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    16141 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    16201 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    16261 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
    16321 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    16381 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
    16441 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
    16501 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
    16561 gggagaccca agctggctag attaagcgtc tgatgagtcc gtgaggacga aacccggcgt
    16621 accgggtc
//
