LOCUS       sequence-343812-        5880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial co-expression of human SEPT7 and of human SEPT9_i5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, 
            Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn
            A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, 
            Cabantous S, Koenderink GH, Bertin A, Mavrakis M
  TITLE     Insights into animal septins using recombinant human septin octamers
            with distinct SEPT9 isoforms.
  JOURNAL   J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
  PUBMED    34237139
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484."
COMMENT     Sequence Label: pnCS_SEPT7_SEPT9_i5-TEV-Strep
FEATURES             Location/Qualifiers
     source          1..5880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     81..100
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        81..99
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    100..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             138..160
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..1193
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1206..1229
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             1245..1267
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2628..2646)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2642..2689
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2860..3651)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3652..3743)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3791..4529)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(4739..5821)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5783..5802
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(join(5822..5880,1..19))
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
       61 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      121 ttccccacta gtaatatttt gtttaacttt aagaaggaga tatacatatg gccgacaccc
      181 ccagagatgc cgggctcaag caggcgcctg catcacggaa cgagaaggcc ccggtggact
      241 tcggctacgt ggggattgac tccatcctgg agcagatgcg ccggaaggcc atgaagcagg
      301 gcttcgagtt caacatcatg gtggtcgggc agagcggctt gggtaaatcc accttaatca
      361 acaccctctt caaatccaaa atcagccgga agtcggtgca gcccacctca gaggagcgca
      421 tccccaagac catcgagatc aagtccatca cgcacgatat tgaggagaaa ggcgtccgga
      481 tgaagctgac agtgattgac acaccagggt tcggggacca catcaacaac gagaactgct
      541 ggcagcccat catgaagttc atcaatgacc agtacgagaa atacctgcag gaggaggtca
      601 acatcaaccg caagaagcgc atcccggaca cccgcgtcca ctgctgcctc tacttcatcc
      661 ccgccaccgg ccactccctc aggcccctgg acatcgagtt tatgaaacgc ctgagcaagg
      721 tggtcaacat cgtccctgtc atcgccaagg cggacacact caccctggag gagagggtcc
      781 acttcaaaca gcggatcacc gcagacctgc tgtccaacgg catcgacgtg tacccccaga
      841 aggaatttga tgaggactcg gaggaccggc tggtgaacga gaagttccgg gagatgatcc
      901 catttgctgt ggtgggcagt gaccacgagt accaggtcaa cggcaagagg atccttggga
      961 ggaagaccaa gtggggtacc atcgaagttg aaaacaccac acactgtgag tttgcctacc
     1021 tgcgggacct tctcatcagg acgcacatgc agaacatcaa ggacatcacc agcagcatcc
     1081 acttcgaggc gtaccgtgtg aagcgcctca acgagggcag cagcgccatg gccaacggcg
     1141 tggaggagaa ggagccagaa gccccagaga tggaaaacct ttacttccag ggcagcggca
     1201 gcggctggag ccacccgcag ttcgaaaaat aaactagtaa taattttgtt taactttaag
     1261 aaggagatat acatatgtcg gtcagtgcga gatccgctgc tgctgaggag aggagcgtca
     1321 acagcagcac catggtagct caacagaaga accttgaagg ctatgtggga tttgccaatc
     1381 tcccaaatca agtatacaga aaatcggtga agagaggttt tgaattcacg cttatggtag
     1441 tgggtgaatc tggattggga aagtcgacat taatcaactc attattcctc acagatttgt
     1501 attctccaga gtatccaggt ccttctcata gaattaaaaa gactgtacag gtggaacaat
     1561 ccaaagtttt aatcaaagaa ggtggtgttc agttgctgct cacaatagtt gataccccag
     1621 gatttggaga tgcagtggat aatagtaatt gctggcagcc tgttatcgac tacattgata
     1681 gtaaatttga ggactaccta aatgcagaat cacgagtgaa cagacgtcag atgcctgata
     1741 acagggtgca gtgttgttta tacttcattg ctccttcagg acatggactt aaaccattgg
     1801 atattgagtt tatgaagcgt ttgcatgaaa aagtgaatat catcccactt attgccaaag
     1861 cagacacact cacaccagag gaatgccaac agtttaaaaa acagataatg aaagaaatcc
     1921 aagaacataa aattaaaata tacgaatttc cagaaacaga tgatgaagaa gaaaataaac
     1981 ttgttaaaaa gataaaggac cgtttacctc ttgctgtggt aggtagtaat actatcattg
     2041 aagttaatgg caaaagggtc agaggaaggc agtatccttg gggtgttgct gaagttgaaa
     2101 atggtgaaca ttgtgatttt acaatcctaa gaaatatgtt gataagaaca cacatgcagg
     2161 acttgaaaga tgttactaat aatgtccact atgagaacta cagaagcaga aaacttgcag
     2221 ctgtgactta taatggagtt gataacaaca agaataaagg gcagctgact aagagccctc
     2281 tggcacaaat ggaagaagaa agaagggagc atgtagctaa aatgaagaag atggagatgg
     2341 agatggagca ggtgtttgag atgaaggtca aagaaaaagt tcaaaaactg aaggactctg
     2401 aagctgagct ccagcggcgc catgagcaaa tgaaaaagaa tttggaagca cagcacaaag
     2461 aattggagga aaaacgtcgt cagttcgagg atgagaaagc aaactgggaa gctcaacaac
     2521 gtattttaga acaacagaac tcttcaagaa ccttggaaaa gaacaagaag aaagggaaga
     2581 tcttttaagg atcctaatag tctagagcta gccctaggct gctgccaccg ctgagcaata
     2641 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
     2701 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     2761 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc
     2821 ggatcttgcg gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
     2881 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     2941 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     3001 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     3061 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     3121 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     3181 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     3241 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     3301 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     3361 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     3421 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     3481 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     3541 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     3601 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct
     3661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3721 atgtatttag aaaaataaac aaatagctag gtcactcggt cgctacgctc cgggcgtgag
     3781 actgcggcgg gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg
     3841 gactaacatg tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc
     3901 cctcctgcca gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg
     3961 ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt
     4021 tccggcgggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     4081 gttccgcctt tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc
     4141 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
     4201 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta
     4261 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     4321 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     4381 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     4441 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     4501 aaaaaggatc tcaagaagat cctttgatct tttctactga accgctcttg atttcagtgc
     4561 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct
     4621 catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     4681 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     4741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4801 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     4861 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     4921 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     4981 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc
     5041 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     5101 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     5161 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     5221 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     5281 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     5341 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     5401 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     5461 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     5521 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     5581 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     5641 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     5701 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     5761 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     5821 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
//
