LOCUS       sequence-343813-        6699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial co-expression of human SEPT7 and of human SEPT9_i1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, 
            Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn
            A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, 
            Cabantous S, Koenderink GH, Bertin A, Mavrakis M
  TITLE     Insights into animal septins using recombinant human septin octamers
            with distinct SEPT9 isoforms.
  JOURNAL   J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
  PUBMED    34237139
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484."
COMMENT     Sequence Label: pnCS_SEPT7_SEPT9_i1-TEV-Strep
FEATURES             Location/Qualifiers
     source          1..6699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    48..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(72..91)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        153..230
                     /gene="lacI"
                     /label=lacI promoter
     CDS             231..1313
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(250..269)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        2374..2465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2466..3257
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(3428..3475)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     3471..3489
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     RBS             4850..4872
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(4888..4911)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(4924..4944)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catatgtata tctccttctt aaagttaaac aaaattatta ctagtgggga attgttatcc
       61 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcctaatgca
      121 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
      181 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
      241 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
      301 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
      361 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
      421 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
      481 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
      541 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
      601 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
      661 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
      721 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
      781 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
      841 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
      901 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
      961 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     1021 ttggtgcgga catctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     1081 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     1141 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     1201 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1261 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     1321 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
     1381 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactaacat gagaattaca
     1441 acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa
     1501 tcaagagcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1561 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     1621 cagttcccca actgacttaa ccttcgaaaa aaccacctgg ggaggtggtt tgatcgaagg
     1681 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
     1741 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
     1801 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttacc
     1861 gtgcttttcc gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg
     1921 aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata
     1981 ccagtgtgtg agctatgaga aagcgccaca cttcccgtaa gggagaaagg cggaacaggt
     2041 atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgaccc gccggaaacg
     2101 gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg gttgagcctc
     2161 acggctccca cagatgcacc ggaaaagcgt ctgtttatgt gaactctggc aggagggcgg
     2221 agcctatgga aaaacgccac cggcgcggcc ctgctgtttt gcctcacatg ttagtcccct
     2281 gcttatccac ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc cgcagtctca
     2341 cgcccggagc gtagcgaccg agtgacctag ctatttgttt atttttctaa atacattcaa
     2401 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2461 agagtatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2521 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2581 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2641 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2701 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2761 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2821 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2881 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2941 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3001 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3061 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3121 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3181 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3241 aggtagtcgg caaataatgt ctaacaattc gttcaagccg aggggccgca agatccggcc
     3301 acgatgaccc ggtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct
     3361 ggtttaccgg tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag
     3421 gtttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt
     3481 gctcagcggt ggcagcagcc tagggctagc tctagactat taggatcctt aaaagatctt
     3541 ccctttcttc ttgttctttt ccaaggttct tgaagagttc tgttgttcta aaatacgttg
     3601 ttgagcttcc cagtttgctt tctcatcctc gaactgacga cgtttttcct ccaattcttt
     3661 gtgctgtgct tccaaattct ttttcatttg ctcatggcgc cgctggagct cagcttcaga
     3721 gtccttcagt ttttgaactt tttctttgac cttcatctca aacacctgct ccatctccat
     3781 ctccatcttc ttcattttag ctacatgctc ccttctttct tcttccattt gtgccagagg
     3841 gctcttagtc agctgccctt tattcttgtt gttatcaact ccattataag tcacagctgc
     3901 aagttttctg cttctgtagt tctcatagtg gacattatta gtaacatctt tcaagtcctg
     3961 catgtgtgtt cttatcaaca tatttcttag gattgtaaaa tcacaatgtt caccattttc
     4021 aacttcagca acaccccaag gatactgcct tcctctgacc cttttgccat taacttcaat
     4081 gatagtatta ctacctacca cagcaagagg taaacggtcc tttatctttt taacaagttt
     4141 attttcttct tcatcatctg tttctggaaa ttcgtatatt ttaattttat gttcttggat
     4201 ttctttcatt atctgttttt taaactgttg gcattcctct ggtgtgagtg tgtctgcttt
     4261 ggcaataagt gggatgatat tcactttttc atgcaaacgc ttcataaact caatatccaa
     4321 tggtttaagt ccatgtcctg aaggagcaat gaagtataaa caacactgca ccctgttatc
     4381 aggcatctga cgtctgttca ctcgtgattc tgcatttagg tagtcctcaa atttactatc
     4441 aatgtagtcg ataacaggct gccagcaatt actattatcc actgcatctc caaatcctgg
     4501 ggtatcaact attgtgagca gcaactgaac accaccttct ttgattaaaa ctttggattg
     4561 ttccacctgt acagtctttt taattctatg agaaggacct ggatactctg gagaatacaa
     4621 atctgtgagg aataatgagt tgattaatgt cgactttccc aatccagatt cacccactac
     4681 cataagcgtg aattcaaaac ctctcttcac cgattttctg tatacttgat ttgggagatt
     4741 ggcaaatccc acatagcctt caaggttctt ctgttgagct accatggtgc tgctgttgac
     4801 gctcctctcc tcagcagcag cggatctcgc actgaccgac atatgtatat ctccttctta
     4861 aagttaaaca aaattattac tagtttattt ttcgaactgc gggtggctcc agccgctgcc
     4921 gctgccctgg aagtaaaggt tttccatctc tggggcttct ggctccttct cctccacgcc
     4981 gttggccatg gcgctgctgc cctcgttgag gcgcttcaca cggtacgcct cgaagtggat
     5041 gctgctggtg atgtccttga tgttctgcat gtgcgtcctg atgagaaggt cccgcaggta
     5101 ggcaaactca cagtgtgtgg tgttttcaac ttcgatggta ccccacttgg tcttcctccc
     5161 aaggatcctc ttgccgttga cctggtactc gtggtcactg cccaccacag caaatgggat
     5221 catctcccgg aacttctcgt tcaccagccg gtcctccgag tcctcatcaa attccttctg
     5281 ggggtacacg tcgatgccgt tggacagcag gtctgcggtg atccgctgtt tgaagtggac
     5341 cctctcctcc agggtgagtg tgtccgcctt ggcgatgaca gggacgatgt tgaccacctt
     5401 gctcaggcgt ttcataaact cgatgtccag gggcctgagg gagtggccgg tggcggggat
     5461 gaagtagagg cagcagtgga cgcgggtgtc cgggatgcgc ttcttgcggt tgatgttgac
     5521 ctcctcctgc aggtatttct cgtactggtc attgatgaac ttcatgatgg gctgccagca
     5581 gttctcgttg ttgatgtggt ccccgaaccc tggtgtgtca atcactgtca gcttcatccg
     5641 gacgcctttc tcctcaatat cgtgcgtgat ggacttgatc tcgatggtct tggggatgcg
     5701 ctcctctgag gtgggctgca ccgacttccg gctgattttg gatttgaaga gggtgttgat
     5761 taaggtggat ttacccaagc cgctctgccc gaccaccatg atgttgaact cgaagccctg
     5821 cttcatggcc ttccggcgca tctgctccag gatggagtca atccccacgt agccgaagtc
     5881 caccggggcc ttctcgttcc gtgatgcagg cgcctgcttg agcccggcat ctctgggggt
     5941 gtcggccatg tcgccaacgc agctgggagc cgcctcagtg gcctcctggc tccggggtgt
     6001 agcctcagcc acagggggct ggggcttggg ctccagcctg ctctgcagct gagacacagg
     6061 ggcaggctct gaattctcca aggtctgggc tgggctgggg gcggtgggcg cctcagcagg
     6121 cttgggcatc tggatctcca ccctcttggg ggctgcgtcg gtggcagggg cagtgggcac
     6181 ctcggggacc ttggaggcag ggggctccat cctccggtgg gctgactcct ggggtttgac
     6241 gatggtgatc tccgtcctcc gaggggccgg ttctggcgtc ttgtggccca acacctcggc
     6301 cctcttgagc ccgaaccggg acgggccgat ggccccggcg ttctccacct gcttggacga
     6361 gatgtcaatg gacagctcag tgcgccggga caccggctcg gccgccttgg ggcccgagag
     6421 ctccaccctc cgcagggacg ccttggggga gcgttggctt agggagtcca catggcgggc
     6481 cgagggttct gagttcttca cgcccaggtc ctggaatttc tgggtggagc tggccacagt
     6541 ggctcggagt aggggagtct ggaccctccg gggtggggtg gagttgggtg tctcgacctc
     6601 ctcgacctca aaagatcttt ttaaggctgg gccactggag tcaccaagcc tccggagccg
     6661 gccactggag gtccgcgtgc ctcctgagta agacttctt
//
