LOCUS       sequence-343829-       10985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Paz MM, Martinez JC, Kalvig AB, Fonger TM, Wang K
  TITLE     Improved cotyledonary node method using an alternative explant 
            derived from mature seed for efficient Agrobacterium-mediated 
            soybean transformation.
  JOURNAL   Plant Cell Rep. 2006 Mar;25(3):206-13. doi: 
            10.1007/s00299-005-0048-7. Epub 2005 Oct 25.
  PUBMED    16249869
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00299-005-0048-7"; journalName: "Plant Cell Rep"; date: 
            "2006-03"; volume: "25"; issue: "3"; pages: "206-13"
COMMENT     Sequence Label: pTF101.1gw3
FEATURES             Location/Qualifiers
     source          1..10985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    50..173
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(214..519)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(316..335)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(839..1519)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     1434..1453
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1573..1579,1580..1597,1598..1603))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1628..1751)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1759..1775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1868..1884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1881..1903)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1892..1908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1916..1922,1923..1940,1941..1946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1961..1982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2140..2164
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3495..4124
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4553..5626
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5692..5886
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6122..6144)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6230..6370
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6285..6304
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6385..6402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6556..7144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6636..6655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7390..8181)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8702..8726
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     8941..8957
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9527..10075)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10227..10571)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10263..10283)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gcaagctaag cttcaggaaa cagctatgac catgattacg ccaagctatc aactttgtat
       61 agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggtcgacc
      181 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
      241 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
      301 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      361 caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg ccagggggat
      421 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
      481 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      541 cggcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      601 ccgctttccg gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttaccg
      661 aaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
      721 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
      781 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
      841 agtaagccgg atcctctaga ttacgccccg cctgccactc atcgcagtac tgttgtaatt
      901 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      961 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1021 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1081 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1141 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1201 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1261 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     1321 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1381 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1441 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1501 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgac ggatcctaac
     1561 tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1621 cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     1681 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttatt
     1741 atacatagtt gataattcac tggccgtcgt tttacaacgt cgtgactggg aaaacaagct
     1801 ttcagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcg
     1861 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1921 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1981 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2041 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggagcttga gcttggatca
     2101 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     2161 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     2221 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     2281 tactttaaag tactttaaag tactttaaag tactttgatc caacccctcc gctgctatag
     2341 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     2401 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2461 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2521 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     2581 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     2641 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     2701 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     2761 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     2821 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     2881 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     2941 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3001 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3061 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3121 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3181 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3241 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     3301 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3361 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3421 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     3481 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3541 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     3601 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     3661 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     3721 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     3781 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     3841 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     3901 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     3961 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4021 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4081 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4141 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4201 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4261 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4321 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4381 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4441 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4501 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     4561 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     4621 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     4681 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     4741 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     4801 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     4861 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     4921 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     4981 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     5041 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     5101 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     5161 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     5221 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     5281 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     5341 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     5401 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     5461 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     5521 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     5581 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     5641 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     5701 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     5761 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     5821 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     5881 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     5941 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     6001 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     6061 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     6121 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6241 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6301 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6361 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6961 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7201 ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt attatgcacg
     7261 cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta
     7321 gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaatttct
     7381 agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     7441 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     7501 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     7561 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     7621 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     7681 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     7741 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     7801 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     7861 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     7921 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     7981 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     8041 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     8101 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     8161 ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     8221 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     8281 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
     8341 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     8401 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcccg
     8461 aattgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
     8521 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
     8581 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
     8641 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
     8701 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
     8761 ggacgttttt aatgtactga attaacgccg aattgctcta gcattcgcca ttcaggctgc
     8821 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     8881 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     8941 gtaaaacgac ggccagtgcc aagctaattc gcttcaagac gtgctcaaat cactatttcc
     9001 acacccctat atttctattg cactcccttt taactgtttt ttattacaaa aatgccctgg
     9061 aaaatgcact ccctttttgt gtttgttttt ttgtgaaacg atgttgtcag gtaatttatt
     9121 tgtcagtcta ctatggtggc ccattatatt aatagcaact gtcggtccaa tagacgacgt
     9181 cgattttctg catttgttta accacgtgga ttttatgaca ttttatatta gttaatttgt
     9241 aaaacctacc caattaaaga cctcatatgt tctaaagact aatacttaat gataacaatt
     9301 ttcttttagt gaagaaaggg ataattagta aatatggaac aagggcagaa gatttattaa
     9361 agccgcgtaa gagacaacaa gtaggtacgt ggagtgtctt aggtgactta cccacataac
     9421 ataaagtgac attaacaaac atagctaatg ctcctatttg aatagtgcat atcagcatac
     9481 cttattacat atagatagga gcaaactcta gctagattgt tgagcagatc tcggtgacgg
     9541 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
     9601 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
     9661 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
     9721 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
     9781 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
     9841 ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
     9901 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
     9961 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
    10021 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag
    10081 atcccccgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta aaagaaatga
    10141 tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt gttttgtata
    10201 tgttgtgttg agaattaatt ctcgaggtcc tctccaaatg aaatgaactt ccttatatag
    10261 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    10321 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    10381 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
    10441 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
    10501 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
    10561 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
    10621 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
    10681 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    10741 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
    10801 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
    10861 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
    10921 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca
    10981 ggcat
//
