LOCUS       sequence-343831-        7925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses D. melanogaster Drp1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
  TITLE     Dynamin-related protein 1 has membrane constricting and severing 
            abilities sufficient for mitochondrial and peroxisomal fission.
  JOURNAL   Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
  PUBMED    30531964
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-7"; volume: "9(1):5239. doi"; pages: " 
            10.1038/s41467-018-07543-w"
COMMENT     Sequence Label: 6xHis(TEV)-dDrp1-StrepII
FEATURES             Location/Qualifiers
     source          1..7925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1281..2363)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2325..2344
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2364..2441)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2647..2666
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2750..2769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2750..2768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2769..2793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2830
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2849..2866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2867..2887
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5099..5122
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5175..5193)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5189..5236
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5411..5416,5417..5433,5434..5439))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(5466..5484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5552..5656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5657..5725,5726..6517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5875..5894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6688..7276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7177..7196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7430..7447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7462..7601
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7527..7546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7687..7709
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7703..7894)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
       61 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
      121 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
      181 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
      241 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
      301 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
      361 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
      421 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
      481 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
      541 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
      601 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
      661 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
      721 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
      781 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
      841 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
      901 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
      961 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     1021 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     1081 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     1141 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     1201 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     1261 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     1381 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     1441 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     1501 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     1561 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     1621 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     1681 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     1741 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     1801 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     1861 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     1921 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     1981 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     2041 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     2101 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     2161 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     2221 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     2281 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     2341 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     2401 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     2461 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     2521 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     2581 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     2641 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     2701 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     2761 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
     2821 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacgaaa atctgtattt
     2881 ccaaggctcg atggaggcac taattccggt cataaacaag ctgcaggacg tcttcaacac
     2941 cgtgggctcc gactccatcc aattgcccca aattgttgtt ctaggcagtc agagctccgg
     3001 caagagttcg gtgatcgaga gcgttgtggg acgatccttt ctgccccgtg gaactggtat
     3061 tgtgacccgt cgcccactgg tgctccagct gatctacagc ccactcgatg atcgcgagaa
     3121 tcgctcggcg gaaaatggta cgtccaatgc cgaggagtgg ggccgcttcc tgcacaccaa
     3181 gaagtgcttc acggactttg acgagatccg caaggagatc gaaaacgaaa cggagcgggc
     3241 ggcgggcagc aacaagggca tctgcccgga gcccatcaac ctgaagatat tctcgacgca
     3301 cgtggtcaac ctaacgctag tggatctgcc cggcatcacc aaggtcccgg tgggcgatca
     3361 gccggaggat atcgaggcgc agatcaaaga attggtcctc aagtacattg agaacccgaa
     3421 ctccatcatt ttggctgtga cggccgccaa cacggacatg gccaccagtg aggcactcaa
     3481 gctggccaag gacgtggacc ccgatggccg gcgcactctg gccgtggtca cgaaactgga
     3541 tctcatggat gccggcaccg atgccattga catcctgtgc ggccgggtca ttcccgttaa
     3601 gctgggcatc atcggcgtga tgaatcgctc gcagaaggac atcatggacc aaaagcacat
     3661 cgacgatcag atgaaggacg aggccgcctt cctgcagcgc aagtatccca ctctggccac
     3721 ccggaacggc acgccctact tggccaagac cctcaaccgg ctcctgatgc accacatccg
     3781 ggactgcctg ccagatctta agactcgcgt caacatcatg gccacgcaat tccaatcgct
     3841 gctaaactcc tatggcgagg atgtctccga caagagccag accctgctgc agatcatcac
     3901 gaagttctcc agcgcctact gctgcacaat cgagggcacg gccaggaata tcgagaccac
     3961 ggaactgtgc ggcggtgccc ggatgggcta cattttccac gagaccttcg gccgcaccct
     4021 ggactccatt cacccgctgg cgggtctgag caagatggac atactgacag ccatccggaa
     4081 tgccactggt ccacgcccgg ctttgttcgt tccggaggtc tcgttcgaac tgctagtgaa
     4141 gcgccagatt cgccgcctgg aggaaccctc cttgcgctgc gtggaactca ttcacgagga
     4201 gatgcagcgc attgttcagc attgtggcaa cgaagtgcag caggagatgc tgcgcttccc
     4261 caaactgcac gagaagattg tggacgtggt cacccagctg ctgcgtcgcc gtctgccgca
     4321 caccaacgtg atggtggaga acattgtggc catcgagctg gcctacatca acaccaagca
     4381 ccccgacttc cacaaggatg ccgctttggt gcccagtctg ctgaaaacgg acagcgatcc
     4441 ctactcacag ataaatctgg gccagcggcg ggccaataca ccgcgcaacc acatgtcgcc
     4501 tcagatttcg tcgcacagtg ctggttcaca gcagccgcag cagcagcagc cgccgcagcc
     4561 gagttcctca cagcagcaat atagccaagt gcatgagcag aatcacgtcg ccgagaactc
     4621 gacgccttcg atggcgagca catggctgtc gaacatttta ccgccggcac caacgcgtcc
     4681 ggacagcatt gagaactcga ccaacaacac tccggttcac aataacatcg tgagtccagt
     4741 gaagccggtc aatctgctgc ccgatgtgcc ggccaaccac aatccacgtc gcctcactga
     4801 caaggagcag aaggattgcg atgtcatcga acacctgatc aaatcgtatt tctacattgt
     4861 gcgcaaatcc atacaagact cggttccaaa ggccatcatg catttcttgg ttaactacgt
     4921 taaggacaac ttgcagagcg aattggttac acatctgtat aagtcggata aggctgaaac
     4981 cctgctcaac gagtccgatc acattgcagt gcgccgaaaa gaggcggccg atatgctgaa
     5041 ggcactgacg cgggcgaatc acatcatcag cgaaatccgc gaaactcaca tgtggctatg
     5101 gtctcatcct cagttcgaaa aataagatcc ggctgctaac aaagcccgaa aggaagctga
     5161 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     5221 cttgaggggt tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc
     5281 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
     5341 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
     5401 aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
     5461 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5521 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5581 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5641 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5701 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5761 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5821 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5881 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5941 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6001 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6061 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6121 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6181 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     6241 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6301 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6361 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6421 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6481 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6541 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6601 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6661 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7261 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7321 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7381 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7441 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7501 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     7561 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     7621 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7681 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     7741 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     7801 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     7861 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     7921 tgttc
//
