LOCUS       sequence-343832-       12102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRO6p::GUSPlus::tOCS in pFP100.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI006
FEATURES             Location/Qualifiers
     source          1..12102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(1294..1308,1499..3316)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          1309..1498
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             3323..3340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3353..4060
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(4303..5013,5014..5016,5017..5019))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4303..5013,5014..5016,5017..5019))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4345..4366)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4694..4713
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4953..4974
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5655..5679
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6200..6991
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7237..7825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7726..7745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7979..7996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8011..8151
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8077..8096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8237..8259
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8495..8689
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8755..9828)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10257..10886)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctaagct cgatgctctc aaggccaaag aacattatgg aaattcgaag
      241 atatattatc gatgcatata atttatataa tcacagtctg tatgtattat ggaaacaact
      301 ttaactaatt tttgagattg atgctacttc atgatagcaa tttcttagtt cttttgagtt
      361 aataacttaa tatataaata taatggtaca acaaccatta caatcgtcat tataatcatc
      421 ttatccatac tcaatagtca atactctcac cttccctaaa gtgtggttga aatcaaactt
      481 tactctctct aggcctgatt ataattcttt tttgtctaca catacaactt catgagactt
      541 aattataatt tcttagtgtt cttttgttga catgtaaatc gtagctaagt ctaagtggta
      601 cttttatttg ttattggatt ttgtttcggt tacttaaaag tgtaatgaag aatttttttg
      661 actatataca tctaagtttt tccttggact gattataact tgtgtgttcg agattgatac
      721 aacttcatga tagtcgatta ttcttgttta acgtaacaca ttgttagata gtgtcattta
      781 ccagaaacaa tctaacagtg aaactttgca aatttatctc tctgctggat cttgaacgcg
      841 gaaaccgaat aatgtttctt tcgagctaat ccgaatgatg ttgaaattct atatgttcag
      901 attcaggtaa tgtaagctta tccaataacc atcatagtcg tcataataac catcttatct
      961 atactaaagt agtaagtact ctcagtctct caccttaccc ttaattgtgg ttgaaatcaa
     1021 cctaacgtga cactctctcc caagtacgtt actctcacgt gttaatgaca ctctcatttt
     1081 tttttatagt tttttttatt gtgtactagg attgttgtcc gaaggaatct ttcataaatg
     1141 agaatgatag aaacctcaat ggtccagaaa attttacttg actcttctaa gaatatctta
     1201 ttagtttatc tctagtaacg ccaccttaaa agccgatgct gcttctgagc caacttctca
     1261 tcacaacact gagaagtgga aattcaaata aagatggtag atctgagggt aaatttctag
     1321 tttttctcct tcattttctt ggttaggacc cttttctctt tttatttttt tgagctttga
     1381 tctttcttta aactgatcta ttttttaatt gattggttat ggtgtaaata ttacatagct
     1441 ttaactgata atctgattac tttatttcgt gtgtctatga tgatgatgat agttacagaa
     1501 ccgacgaact agtctgtacc cgatcaacac cgagacccgt ggcgtcttcg acctcaatgg
     1561 cgtctggaac ttcaagctgg actacgggaa aggactggaa gagaagtggt acgaaagcaa
     1621 gctgaccgac actattagta tggccgtccc aagcagttac aatgacattg gcgtgaccaa
     1681 ggaaatccgc aaccatatcg gatatgtctg gtacgaacgt gagttcacgg tgccggccta
     1741 tctgaaggat cagcgtatcg tgctccgctt cggctctgca actcacaaag caattgtcta
     1801 tgtcaatggt gagctggtcg tggagcacaa gggcggattc ctgccattcg aagcggaaat
     1861 caacaactcg ctgcgtgatg gcatgaatcg cgtcaccgtc gccgtggaca acatcctcga
     1921 cgatagcacc ctcccggtgg ggctgtacag cgagcgccac gaagagggcc tcggaaaagt
     1981 cattcgtaac aagccgaact tcgacttctt caactatgca ggcctgcacc gtccggtgaa
     2041 aatctacacg accccgttta cgtacgtcga ggacatctcg gttgtgaccg acttcaatgg
     2101 cccaaccggg actgtgacct atacggtgga ctttcaaggc aaagccgaga ccgtgaaagt
     2161 gtcggtcgtg gatgaggaag gcaaagtggt cgcaagcacc gagggcctga gcggtaacgt
     2221 ggagattccg aatgtcatcc tctgggaacc actgaacacg tatctctacc agatcaaagt
     2281 ggaactggtg aacgacggac tgaccatcga tgtctatgaa gagccgttcg gcgtgcggac
     2341 cgtggaagtc aacgacggca agttcctcat caacaacaaa ccgttctact tcaagggctt
     2401 tggcaaacat gaggacactc ctatcaacgg ccgtggcttt aacgaagcga gcaatgtgat
     2461 ggatttcaat atcctcaaat ggatcggcgc caacagcttc cggaccgcac actatccgta
     2521 ctctgaagag ttgatgcgtc ttgcggatcg cgagggtctg gtcgtgatcg acgagactcc
     2581 ggcagttggc gtgcacctca acttcatggc caccacggga ctcggcgaag gcagcgagcg
     2641 cgtcagtacc tgggagaaga ttcggacgtt tgagcaccat caagacgttc tccgtgaact
     2701 ggtgtctcgt gacaagaacc atccaagcgt cgtgatgtgg agcatcgcca acgaggcggc
     2761 gactgaggaa gagggcgcgt acgagtactt caagccgttg gtggagctga ccaaggaact
     2821 cgacccacag aagcgtccgg tcacgatcgt gctgtttgtg atggctaccc cggagacgga
     2881 caaagtcgcc gaactgattg acgtcatcgc gctcaatcgc tataacggat ggtacttcga
     2941 tggcggtgat ctcgaagcgg ccaaagtcca tctccgccag gaatttcacg cgtggaacaa
     3001 gcgttgccca ggaaagccga tcatgatcac tgagtacggc gcagacaccg ttgcgggctt
     3061 tcacgacatt gatccagtga tgttcaccga ggaatatcaa gtcgagtact accaggcgaa
     3121 ccacgtcgtg ttcgatgagt ttgagaactt cgtgggtgag caagcgtgga acttcgcgga
     3181 cttcgcgacc tctcagggcg tgatgcgcgt ccaaggaaac aagaagggcg tgttcactcg
     3241 tgaccgcaag ccgaagctcg ccgcgcacgt ctttcgcgag cgctggacca acattccaga
     3301 tttcggctac aagaacgcta gccatcacca tcaccatcac gtgtgaagga tcctgcttta
     3361 atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt
     3421 tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat
     3481 gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga
     3541 ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg tgctgaatag
     3601 gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt ttattattac
     3661 aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga ttacataaat
     3721 cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg agcggcgaac
     3781 taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt gagattcctt
     3841 gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca acccggtcca
     3901 gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt ttttggtgta
     3961 tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc gttgggcggg
     4021 tccagggcga attttgcgac aacatgtcga ggctcagcag gacctctaga gcgccactgg
     4081 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     4141 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataagaacc ctaattccct
     4201 tatctgggaa ctactcacac attattatag agagagatag atttgtagag agagactggt
     4261 gatttcagcg atgcagccgg gcggcgctta cagctcgtcc ttcttgtaca gctcgtccat
     4321 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     4381 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     4441 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     4501 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     4561 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     4621 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     4681 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     4741 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     4801 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     4861 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     4921 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     4981 caccaccccg gtgaacagct cctcgccctt gctcaccatg tcggccgagg ataatgatag
     5041 gagaagtgaa aagatgagaa agagaaaaag attagtcttc accatggcta tcgttcgtaa
     5101 atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga aatgatttaa attgctgcaa
     5161 tagaagtaga atgcttgatt gcttgagatt cgtttgtttt gtatatgttg tgttgagaat
     5221 tcgcccttgt tttgctattt gtgtatgttt tcttgttttg gtttgagtga agagtgtagt
     5281 gaagggttgg tttgtattta taggtgagaa aggagatttg catggcaatc acgtgtaaga
     5341 atgcatgcat gcatgagtgt gtgtggagag agagagatgc tcttacgttg ttgtcgggat
     5401 tatgtgttgg aaaagagtga caagtgacga acaaaacaat accactcatc atagctccgc
     5461 ttatgcatgc tatgtgtgat ttgtagctct tgttctgaca tgacactatg aacaattttt
     5521 taaaaagaag gttaaacttt tatttaaata aaaaccctat gttaatttgg tttcaagggc
     5581 gaattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5641 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5701 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5761 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5821 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5881 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5941 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     6001 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     6061 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     6121 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     6181 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     6241 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6301 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     6361 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     6421 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     6481 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     6541 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     6601 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6661 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6721 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6781 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6841 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6901 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6961 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     7021 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     7081 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     7141 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7981 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8041 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     8101 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8281 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     8341 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     8401 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     8461 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     8521 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     8581 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     8641 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8701 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8761 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8821 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8881 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8941 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     9001 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     9061 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     9121 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     9181 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     9241 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     9301 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     9361 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     9421 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     9481 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     9541 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     9601 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9661 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9721 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9781 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     9841 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9901 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9961 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    10021 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    10081 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    10141 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    10201 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    10261 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    10321 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    10381 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    10441 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    10501 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    10561 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    10621 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    10681 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    10741 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    10801 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    10861 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10921 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10981 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    11041 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    11101 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    11161 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    11221 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    11281 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    11341 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    11401 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    11461 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    11521 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    11581 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    11641 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    11701 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    11761 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    11821 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    11881 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11941 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    12001 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    12061 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ct
//
