LOCUS       sequence-343833-       16527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGH3.17::GUSPlus::tOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 16527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI012
FEATURES             Location/Qualifiers
     source          1..16527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(5719..5733,5924..7741)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          5734..5923
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             7748..7765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7778..8485
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(8728..9438,9439..9441,9442..9444))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8728..9438,9439..9441,9442..9444))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8770..8791)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9119..9138
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9378..9399
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    10080..10104
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10625..11416
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11662..12250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12151..12170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12404..12421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12436..12576
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12502..12521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12662..12684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12920..13114
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13180..14253)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14682..15311)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctaagct cataaacatt tacctttcat ggggattaca tttttaagtc
      241 tatagctata tccattattc agttatgtgc ataaatcagc ttcttcttct tcttcttttg
      301 taactaaagc atgcataata agaaagccga tattggacat agaccaacaa agctatgtaa
      361 ttggaatgat cagccaatta ggtaacacat caaagaaaag aaataataga tcctccgatg
      421 tttggtaaat ttattaataa ttatttacac aagttggtaa ttgtggaata atttgtctac
      481 acaaggttga caattgtggc tgagcgatta tgttagagtg catcaagttg aaagtgtagg
      541 attttgttgg actcttttgg tgccttggta aaataagatg atataatcgg ggtggttttg
      601 attgagcttc caccaagata gagtgttcat attttattag ctagtagact taaagattta
      661 tgtcactcat cgtaatgaga aatttctaca atttccactt atatacaatg caaaacaaac
      721 taaattggta caacaaacat atattcatgt gaacctcata tgttcctcat gcatagttac
      781 caatagttcg tatctctcgt cttttattgg gagttatctt atcaatgtgc tgaatttggc
      841 tataatgatg tttttcaaat tgagcacacg accatgagta ctttattttt tgttttttca
      901 aaatgactta aaatcgttgt gtatcattta tcgagtctct attcgaaagg ccagactaac
      961 tcgatttgga ctggtgccaa gacaaaagct tgtttttggg gaggtgcgga aaggtgaaac
     1021 cctccgtagt acgtgatgta tctcaggaat agaaatgttc tgattttatc atttaatctt
     1081 tgtggagttt gatgaatata taaaggtgat ttagagtttt tatgtcatta tttacatgat
     1141 tactaaaaga aaccctaggc taaaagatat ttgcctcaaa attgaaaaag ttgaatattt
     1201 taacacttta ctaatatata ctttgttaag caccattttt ccctcctatt ttctctcctt
     1261 atttcaagac tacatgtgcc ctcatagaga cacttgcttt tgcatttcac tttatgttaa
     1321 tctgagcaat aaaagacctc taaagaccat atctaagcaa atccctgaac aaatgtccta
     1381 atcaacaatt taattaatac tagtttcttt tcggtttcaa ggcgtttacc aaaaaccaaa
     1441 caactcttta cacaaaacta gttctattca ctttgggatc ggtgatgtgc ttattgctat
     1501 taattaatac tagatatgat catagtctct tctattccaa gcaacgcata ccactttgtt
     1561 tatgtggtac accagtcatt tacttgccta ctcaatagaa gttttcactt ttgattttgt
     1621 atttctctcc ataagtataa tattaggaat aattaaagct atataagtaa agaatgcgaa
     1681 aatcttgatg gatttaacat tatatatata tatatatata tatatctttc tagatttaga
     1741 tttaaactcg gattgttatg caatgacgca actagtataa atgactaggc tacatcttac
     1801 atgttatggt cagctaaaaa ttgttatgtt aaatgtgtga ttaaaattaa tttaattgag
     1861 gtgtatgtac acacgaagga ttttatattg ctatagatag ataaataaat aaaaataatt
     1921 tatactgttt ggactgacca agttataaca ctaagaaaaa caataatatg ggaattccac
     1981 agtttatttt tcactaattt tcaaaatccg ttattttttt ttaaaaaatg ttctaactaa
     2041 acgagagaat taaagttata ttctcttcaa ggaagctggc aaatttattt aaagtctcac
     2101 ttgtacatca aacgtacgtt gaatcattta taagagctga gtgtgtaaga tacgtaattt
     2161 aaatttgaca agtcattagc tttgccaaat atttcgaaca ctatgaagtc tgtgatcatt
     2221 aacttatttt tcttgtcaac agaagtcaca tgttacatca caaatcctaa ttagtagaac
     2281 actagaaatc agatttagct ataaccatcc aacatataag ctatacactt gatttttgga
     2341 gacttgtggg taaactaact actcttgaaa tgtgagactc acatgtaagt cttgtttcgg
     2401 taattaatct ctaaatttta atcttatgta accctacgtt aatagaccga ataatatttt
     2461 ttaacttgag taaatcgaaa tcaatactca tattactaga ttataatctc atatcatatt
     2521 cctaattttt attgtatttt ttctgtttct cttattgcca tttatctgaa attttctttt
     2581 taatttattt tctcttatgg ttttgtcgtg attcaggagt agtaattaat tcacgtcatg
     2641 cgcgatttaa tatgaattta aaaatgttat ctcagtccat gagaagtagt aattgtgttg
     2701 tgcgtattgg tttgaagtag tccaccaaaa gagcaataaa tgcaaagctc gtaactttga
     2761 cctatttttt gtttgtctta ttacactact gatttttttt tcctttgcca tgtctataca
     2821 gtatacacta ttattctctc ttatttttca atatatgaac tacataacac atttaatata
     2881 aacaacatgc attagtttac attagataga aaacttaagc tgttacatga tccaggactt
     2941 tggtatccta gtggcatgtc cgcatgtgtt gcaatctctt aaaactctca aacagcaact
     3001 acaacatatt ctacatagtc tctagatttg tttgggtcaa ccatatagtc tcgaatttaa
     3061 acgtcaaatt cacagtacgt atatggttct taaccactgt atgccaatta ttatcactaa
     3121 ccctttcaat tatactgtat ttgaaacccg aatcttatat taaccttagc gttttaggct
     3181 taagtttatt gttgactttc gaactgttaa catactttta tgggtgtata tatatgactt
     3241 atgctacaac caacttttga tcctttaact tctagcataa gtccatgact gtagtttcca
     3301 atggatatgc ttagaatccc gttgacatat attttgagtg aagaattaga tgatgaacaa
     3361 tataaaagta tttaagcctt ttaacaaaaa tataaaaata tattaaaaaa aaatcctgtt
     3421 aataaattct tttacgggaa tgaccaatca aaatatatgt gggcttattg ttccacgaac
     3481 caggatgaaa cacatcagat gatacatcat tcaaaggttt tgcgaaagtt ctttttatct
     3541 aactaggatt tcggatggtt tgcctaaaat aacataatag aagaaaatat gggcgttacg
     3601 tatcaggaaa atattatcac aaaacaacat ttcaagaatt cattactttt aaaacagaaa
     3661 tagtaaggtt agaaaatatt caaaaagagt gcatgtcttt taaagttgat gggtcggtct
     3721 tatagattgc gcatgtcctg tatctctccc aaacattgtg gaccggtcta tgtatttgtg
     3781 aaaggaaata tgccaacttt tactagtctc tctctcgcat gtgtgtgagt gaaatagttt
     3841 taatatttca aatttatttc caaaacaatt cttacaagga ttatcaactg cgcagcttaa
     3901 aaaatccaat tatactcaac tagtattatc actctatagt tcagaatact tttcttgaaa
     3961 atatgaaata tatataaact ataaatttta tgtttttata ttatttaatg aagacaatca
     4021 atgggattaa gaccattaac gtgtgaaaca taagacatat atgatttatc gtaaggttga
     4081 gaatctatgt atgctttttc agggaccaaa aggaatatat aacatccctt cagaaaaaaa
     4141 aagatttaca tgatttgggt tgttacgttt caaaaagaaa attaaaactc tagtttaaaa
     4201 tctggtagat gtttcagccc cttgtttaat aaatctgaaa cgagattctc accatcattt
     4261 ttttgggata aattatcact attattttca tatatatgtt attttaaatt gattattaat
     4321 tatttagtag aatggtataa tcatgaatta taaacaaata cgtggaataa aataactaaa
     4381 tcgagatgtt taattaaatc aacttaattg tcaaggaaaa gatttatttt agttttatca
     4441 ttaaatgact aaaccaaaat aaactaagca atataaaaag cctaaagcaa aggaattgac
     4501 gaaacatgcc taaagtgtat gacatagcgt atttgaatga aatttgttca cgagaaaaag
     4561 atcatcttct ccaaaccctc tcaggcctct tttctctctg ttcacatctg tttcctcgtc
     4621 acacaagaca tatacattaa cctaattcaa ctgttccatg tcttttaaag ttttcttgat
     4681 ggaattaagc tgatgcatgt tattttatgt tgtaattatc atcatttatg gttagttagt
     4741 caattggtct atttatatta tatgtatatc aacgtaacga ccctatcata tgcatttgga
     4801 tctgcatcac caagatgatg actttataat atccaagaga tttaacaatc ttaacaatcc
     4861 ttctctctct taatcttgat tgccataagt caaacttgag attatttaat tactatatgg
     4921 agcccaatta tcgcatgtaa atgaggttaa tataaatagg cagatttaaa ctaacatata
     4981 tgtcaatgga atttgaataa ctaagagttg tttagtattt cacagcaatg caaatataca
     5041 ggttttaaga acaatatgca aatgaattta agggatatat aaaaagttct taaagaaaat
     5101 tccatagcca catatataaa gaatttgtta acttaagcta tcatatactt ctttttagta
     5161 taaactttat ttgaaaaatt tgcatgtgag ttcataaagg tttcaacgag ttcctaaata
     5221 aaatctagct aagaggcaat attagatgag tatagtgaag accaagtgag tatatctttt
     5281 ggcagacata tgcaaatctt gtgaatatga ttggattgat attattcaac ggatcataaa
     5341 gaaagagctt gattggagga ttaaacagca tataaacaaa tatgatatta aacaaaaaat
     5401 aacatataaa attataggga aagcctgcac aggtaatatt gaccaacaat agcttctttc
     5461 taactagaca aggccatgtg tgttgtagga cctcccaaat atatattttc taataattat
     5521 ttcatcattt gccaatttat tatataacca tttctcatcc ttctcctcat accaccaaaa
     5581 accaaccttc aagaacagag aagaagtgat tctctaaaca acaaaaataa aataaaactc
     5641 ttctttctct atttctgttg aaggtacctc atcattgcaa cacaaactta aagctttgtt
     5701 tgtgtctgct ttcagaaaat ggtagatctg agggtaaatt tctagttttt ctccttcatt
     5761 ttcttggtta ggaccctttt ctctttttat ttttttgagc tttgatcttt ctttaaactg
     5821 atctattttt taattgattg gttatggtgt aaatattaca tagctttaac tgataatctg
     5881 attactttat ttcgtgtgtc tatgatgatg atgatagtta cagaaccgac gaactagtct
     5941 gtacccgatc aacaccgaga cccgtggcgt cttcgacctc aatggcgtct ggaacttcaa
     6001 gctggactac gggaaaggac tggaagagaa gtggtacgaa agcaagctga ccgacactat
     6061 tagtatggcc gtcccaagca gttacaatga cattggcgtg accaaggaaa tccgcaacca
     6121 tatcggatat gtctggtacg aacgtgagtt cacggtgccg gcctatctga aggatcagcg
     6181 tatcgtgctc cgcttcggct ctgcaactca caaagcaatt gtctatgtca atggtgagct
     6241 ggtcgtggag cacaagggcg gattcctgcc attcgaagcg gaaatcaaca actcgctgcg
     6301 tgatggcatg aatcgcgtca ccgtcgccgt ggacaacatc ctcgacgata gcaccctccc
     6361 ggtggggctg tacagcgagc gccacgaaga gggcctcgga aaagtcattc gtaacaagcc
     6421 gaacttcgac ttcttcaact atgcaggcct gcaccgtccg gtgaaaatct acacgacccc
     6481 gtttacgtac gtcgaggaca tctcggttgt gaccgacttc aatggcccaa ccgggactgt
     6541 gacctatacg gtggactttc aaggcaaagc cgagaccgtg aaagtgtcgg tcgtggatga
     6601 ggaaggcaaa gtggtcgcaa gcaccgaggg cctgagcggt aacgtggaga ttccgaatgt
     6661 catcctctgg gaaccactga acacgtatct ctaccagatc aaagtggaac tggtgaacga
     6721 cggactgacc atcgatgtct atgaagagcc gttcggcgtg cggaccgtgg aagtcaacga
     6781 cggcaagttc ctcatcaaca acaaaccgtt ctacttcaag ggctttggca aacatgagga
     6841 cactcctatc aacggccgtg gctttaacga agcgagcaat gtgatggatt tcaatatcct
     6901 caaatggatc ggcgccaaca gcttccggac cgcacactat ccgtactctg aagagttgat
     6961 gcgtcttgcg gatcgcgagg gtctggtcgt gatcgacgag actccggcag ttggcgtgca
     7021 cctcaacttc atggccacca cgggactcgg cgaaggcagc gagcgcgtca gtacctggga
     7081 gaagattcgg acgtttgagc accatcaaga cgttctccgt gaactggtgt ctcgtgacaa
     7141 gaaccatcca agcgtcgtga tgtggagcat cgccaacgag gcggcgactg aggaagaggg
     7201 cgcgtacgag tacttcaagc cgttggtgga gctgaccaag gaactcgacc cacagaagcg
     7261 tccggtcacg atcgtgctgt ttgtgatggc taccccggag acggacaaag tcgccgaact
     7321 gattgacgtc atcgcgctca atcgctataa cggatggtac ttcgatggcg gtgatctcga
     7381 agcggccaaa gtccatctcc gccaggaatt tcacgcgtgg aacaagcgtt gcccaggaaa
     7441 gccgatcatg atcactgagt acggcgcaga caccgttgcg ggctttcacg acattgatcc
     7501 agtgatgttc accgaggaat atcaagtcga gtactaccag gcgaaccacg tcgtgttcga
     7561 tgagtttgag aacttcgtgg gtgagcaagc gtggaacttc gcggacttcg cgacctctca
     7621 gggcgtgatg cgcgtccaag gaaacaagaa gggcgtgttc actcgtgacc gcaagccgaa
     7681 gctcgccgcg cacgtctttc gcgagcgctg gaccaacatt ccagatttcg gctacaagaa
     7741 cgctagccat caccatcacc atcacgtgtg aaggatcctg ctttaatgag atatgcgaga
     7801 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     7861 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     7921 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     7981 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     8041 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     8101 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     8161 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     8221 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     8281 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     8341 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     8401 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     8461 gcgacaacat gtcgaggctc agcaggacct ctagagcgcc actggatttt ggttttagga
     8521 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
     8581 tatgctcaac acatgagcga aaccctataa gaaccctaat tcccttatct gggaactact
     8641 cacacattat tatagagaga gatagatttg tagagagaga ctggtgattt cagcgatgca
     8701 gccgggcggc gcttacagct cgtccttctt gtacagctcg tccatgccga gagtgatccc
     8761 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     8821 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     8881 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     8941 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
     9001 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     9061 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     9121 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     9181 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     9241 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     9301 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     9361 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     9421 cagctcctcg cccttgctca ccatgtcggc cgaggataat gataggagaa gtgaaaagat
     9481 gagaaagaga aaaagattag tcttcaccat ggctatcgtt cgtaaatggt gaaaattttc
     9541 agaaaattgc ttttgcttta aaagaaatga tttaaattgc tgcaatagaa gtagaatgct
     9601 tgattgcttg agattcgttt gttttgtata tgttgtgttg agaattcgcc cttgttttgc
     9661 tatttgtgta tgttttcttg ttttggtttg agtgaagagt gtagtgaagg gttggtttgt
     9721 atttataggt gagaaaggag atttgcatgg caatcacgtg taagaatgca tgcatgcatg
     9781 agtgtgtgtg gagagagaga gatgctctta cgttgttgtc gggattatgt gttggaaaag
     9841 agtgacaagt gacgaacaaa acaataccac tcatcatagc tccgcttatg catgctatgt
     9901 gtgatttgta gctcttgttc tgacatgaca ctatgaacaa ttttttaaaa agaaggttaa
     9961 acttttattt aaataaaaac cctatgttaa tttggtttca agggcgaatt caattcggcg
    10021 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    10081 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    10141 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    10201 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    10261 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    10321 agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt
    10381 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
    10441 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
    10501 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
    10561 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
    10621 catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
    10681 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
    10741 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
    10801 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
    10861 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
    10921 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
    10981 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
    11041 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
    11101 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
    11161 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
    11221 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
    11281 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
    11341 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
    11401 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc
    11461 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
    11521 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
    11581 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11641 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11701 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11761 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    11821 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11881 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11941 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12001 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12061 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    12181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    12241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    12301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    12361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    12421 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    12481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
    12541 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
    12601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    12661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
    12721 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
    12781 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
    12841 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
    12901 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
    12961 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
    13021 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
    13081 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
    13141 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
    13201 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
    13261 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
    13321 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
    13381 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
    13441 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
    13501 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
    13561 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
    13621 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
    13681 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
    13741 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
    13801 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
    13861 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
    13921 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
    13981 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
    14041 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
    14101 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
    14161 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
    14221 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
    14281 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
    14341 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    14401 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    14461 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    14521 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    14581 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    14641 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    14701 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    14761 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    14821 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    14881 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    14941 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    15001 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    15061 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    15121 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    15181 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    15241 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    15301 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    15361 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    15421 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    15481 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    15541 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    15601 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    15661 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    15721 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    15781 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    15841 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    15901 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    15961 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    16021 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    16081 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    16141 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    16201 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    16261 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    16321 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    16381 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    16441 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtacttta
    16501 aagtacttta aagtacttta aagtact
//
