LOCUS       sequence-343834-       10716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (pUBQ10::SMT2-FLAG::tOCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 10716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI046
FEATURES             Location/Qualifiers
     source          1..10716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1935..1958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1967..2674
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(2917..3627,3628..3630,3631..3633))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2917..3627,3628..3630,3631..3633))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2959..2980)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3308..3327
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3567..3588
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4269..4293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4814..5605
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5851..6439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6340..6359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6593..6610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6625..6765
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6691..6710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6851..6873
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7109..7303
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7369..8442)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8871..9500)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctaagct ttcgacgagt cagtaataaa cggcgtcaaa gtggttgcag
      241 ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa
      301 ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt
      361 tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt
      421 cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc
      481 aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt
      541 ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat
      601 atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg
      661 ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata
      721 atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctagt tctggtgtta
      781 gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagacta
      841 gcccgcggaa aatggactct ttaacactct tcttcaccgg tgcactcgtc gccgtcggta
      901 tctactggtt cctctgcgtt ctcggtccag cagagcgtaa aggcaaacga gccgtagatc
      961 tctctggtgg ctcaatctcc gccgagaaag tccaagacaa ctacaaacag tactggtctt
     1021 tcttccgccg tccaaaagaa atcgaaaccg ccgagaaagt tccagacttc gtcgacacat
     1081 tctacaatct cgtcaccgac atatacgagt ggggatgggg acaatccttc cacttctcac
     1141 catcaatccc cggaaaatct cacaaagacg ccacgcgcct ccacgaagag atggccgtag
     1201 atctgatcca agtcaaacct ggtcaaaaga tcctagacgt cggatgcggt gtcggcggtc
     1261 cgatgcgagc gattgcatct cactcgcgag ctaacgtagt cgggattaca ataaacgagt
     1321 atcaggtgaa cagagctcgt ctccacaata agaaagctgg tctcgacgcg ctttgcgagg
     1381 tcgtgtgtgg taacttcctc cagatgccgt tcgatgacaa cagtttcgac ggtgcttatt
     1441 ccatcgaagc cacgtgtcac gcgccgaagc tggaagaagt gtacgcagag atctacaggg
     1501 tgttgaaacc cggatctatg tatgtgtcgt acgagtgggt tacgacggag aaatttaagg
     1561 cggaggatga cgaacacgtg gaggtaatcc aagggattga gagaggcgat gcgttaccag
     1621 ggcttagggc ttacgtggat atagctgaga cggctaaaaa ggttgggttt gagatagtga
     1681 aggagaagga tctggcgagt ccaccggctg agccgtggtg gactaggctt aagatgggta
     1741 ggcttgctta ttggaggaat cacattgtgg ttcagatttt gtcagcggtt ggagttgctc
     1801 ctaaaggaac tgttgatgtt catgagatgt tgtttaagac tgcggattat ttgaccagag
     1861 gaggtgaaac cggaatattc tctccgatgc atatgattct ctgcagaaaa ccggagtcac
     1921 cggaggagag ttctgattac aaggacgacg atgacaagtg aggatcctgc tttaatgaga
     1981 tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa
     2041 aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat
     2101 atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac
     2161 cctactactt atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat
     2221 agcgacatct atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc
     2281 aattttaaaa aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt
     2341 caaatttcaa aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa
     2401 cgctcactga agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt
     2461 gagtattggc cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg
     2521 cggccgggta accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg
     2581 ccccaaatga agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg
     2641 gcgaattttg cgacaacatg tcgaggctca gcaggacctc tagagcgcca ctggattttg
     2701 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     2761 ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg
     2821 ggaactactc acacattatt atagagagag atagatttgt agagagagac tggtgatttc
     2881 agcgatgcag ccgggcggcg cttacagctc gtccttcttg tacagctcgt ccatgccgag
     2941 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     3001 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     3061 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     3121 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     3181 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     3241 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     3301 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     3361 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     3421 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     3481 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     3541 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     3601 cccggtgaac agctcctcgc ccttgctcac catgtcggcc gaggataatg ataggagaag
     3661 tgaaaagatg agaaagagaa aaagattagt cttcaccatg gctatcgttc gtaaatggtg
     3721 aaaattttca gaaaattgct tttgctttaa aagaaatgat ttaaattgct gcaatagaag
     3781 tagaatgctt gattgcttga gattcgtttg ttttgtatat gttgtgttga gaattcgccc
     3841 ttgttttgct atttgtgtat gttttcttgt tttggtttga gtgaagagtg tagtgaaggg
     3901 ttggtttgta tttataggtg agaaaggaga tttgcatggc aatcacgtgt aagaatgcat
     3961 gcatgcatga gtgtgtgtgg agagagagag atgctcttac gttgttgtcg ggattatgtg
     4021 ttggaaaaga gtgacaagtg acgaacaaaa caataccact catcatagct ccgcttatgc
     4081 atgctatgtg tgatttgtag ctcttgttct gacatgacac tatgaacaat tttttaaaaa
     4141 gaaggttaaa cttttattta aataaaaacc ctatgttaat ttggtttcaa gggcgaattc
     4201 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     4261 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     4321 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     4381 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     4441 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     4501 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     4561 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     4621 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     4681 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     4741 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     4801 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     4861 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     4921 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     4981 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     5041 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     5101 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     5161 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     5221 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     5281 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     5341 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     5401 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     5461 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     5521 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     5581 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     5641 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     5701 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     5761 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5821 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5881 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5941 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6001 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6061 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6121 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6181 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6241 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6301 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6361 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6421 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6481 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6541 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6601 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6661 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6721 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6781 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6841 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6901 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     6961 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     7021 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     7081 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     7141 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     7201 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     7261 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     7321 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     7381 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     7441 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     7501 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7561 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7621 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7681 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7741 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7801 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     7861 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     7921 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     7981 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     8041 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     8101 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     8161 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     8221 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     8281 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     8341 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     8401 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     8461 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
     8521 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     8581 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     8641 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     8701 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     8761 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     8821 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     8881 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     8941 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     9001 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     9061 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     9121 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     9181 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     9241 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     9301 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     9361 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     9421 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     9481 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     9541 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     9601 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     9661 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
     9721 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     9781 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
     9841 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
     9901 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
     9961 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    10021 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    10081 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    10141 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    10201 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    10261 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    10321 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    10381 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    10441 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    10501 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    10561 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    10621 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    10681 agtactttaa agtactttaa agtactttaa agtact
//
