LOCUS       sequence-34386-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: NRF2b (nfe212b)_R (OZ562)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..72
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(561..580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatcattgtg caaaggccct gagaaaaatc caagctttgg cggccgctga ctacaaagac
       61 gatgatgaca aatctagacc cggggagcgc cccttccagt gtcgcatttg catgcggaac
      121 ttttcgaagg ccaccaacct tgacaggcat acccgtactc ataccggtga aaaaccgttt
      181 cagtgtcgga tctgtatgcg aaatttctcc aggcccgaca acttgggccg ccatctacgt
      241 acgcacaccg gcgagaagcc attccaatgc cgaatatgca tgcgcaactt cagtcgggag
      301 gacaacctgg ggaggcacct aaaaacccac ctgaggggat cctctgcaca gtagtaacta
      361 gagactagaa aaaggccgac aagtcccgct ccgctgaaga tcctggcgta atagcgaaga
      421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
      481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata a
//
