LOCUS       sequence-343869-        6665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-Spike
FEATURES             Location/Qualifiers
     source          1..6665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3976..4075)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4092..4111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4093..4111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4116..4132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4116..4132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4563..5354
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5447..6035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5936..5955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6189..6206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6365..6392)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6484..6570)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6619..6641
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6633..6650
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6634..6650
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ttgtttttct tgttttattg ccactagtct ctagtcagtg tgttaatctt
      181 acaaccagaa ctcaattacc ccctgcatac actaattctt tcacacgtgg tgtttattac
      241 cctgacaaag ttttcagatc ctcagtttta cattcaactc aggacttgtt cttacctttc
      301 ttttccaatg ttacttggtt ccatgctata catgtctctg ggaccaatgg tactaagagg
      361 tttgataacc ctgtcctacc atttaatgat ggtgtttatt ttgcttccac tgagaagtct
      421 aacataataa gaggctggat ttttggtact actttagatt cgaagaccca gtccctactt
      481 attgttaata acgctactaa tgttgttatt aaagtctgtg aatttcaatt ttgtaatgat
      541 ccatttttgg gtgtttatta ccacaaaaac aacaaaagtt ggatggaaag tgagttcaga
      601 gtttattcta gtgcgaataa ttgcactttt gaatatgtct ctcagccttt tcttatggac
      661 cttgaaggaa aacagggtaa tttcaaaaat cttagggaat ttgtgtttaa gaatattgat
      721 ggttatttta aaatatattc taagcacacg cctattaatt tagtgcgtga tctccctcag
      781 ggtttttcgg ctttagaacc attggtagat ttgccaatag gtattaacat cactaggttt
      841 caaactttac ttgctttaca tagaagttat ttgactcctg gtgattcttc ttcaggttgg
      901 acagctggtg ctgcagctta ttatgtgggt tatcttcaac ctaggacttt tctattaaaa
      961 tataatgaaa atggaaccat tacagatgct gtagactgtg cacttgaccc tctctcagaa
     1021 acaaagtgta cgttgaaatc cttcactgta gaaaaaggaa tctatcaaac ttctaacttt
     1081 agagtccaac caacagaatc tattgttaga tttcctaata ttacaaactt gtgccctttt
     1141 ggtgaagttt ttaacgccac cagatttgca tctgtttatg cttggaacag gaagagaatc
     1201 agcaactgtg ttgctgatta ttctgtccta tataattccg catcattttc cacttttaag
     1261 tgttatggag tgtctcctac taaattaaat gatctctgct ttactaatgt ctatgcagat
     1321 tcatttgtaa ttagaggtga tgaagtcaga caaatcgctc cagggcaaac tggaaagatt
     1381 gctgattata attataaatt accagatgat tttacaggct gcgttatagc ttggaattct
     1441 aacaatcttg attctaaggt tggtggtaat tataattacc tgtatagatt gtttaggaag
     1501 tctaatctca aaccttttga gagagatatt tcaactgaaa tctatcaggc cggtagcaca
     1561 ccttgtaatg gtgttgaagg ttttaattgt tactttcctt tacaatcata tggtttccaa
     1621 cccactaatg gtgttggtta ccaaccatac agagtagtag tactttcttt tgaacttcta
     1681 catgcaccag caactgtttg tggacctaaa aagtctacta atttggttaa aaacaaatgt
     1741 gtcaatttca acttcaatgg tttaacaggc acaggtgttc ttactgagtc taacaaaaag
     1801 tttctgcctt tccaacaatt tggcagagac attgctgaca ctactgatgc tgtccgtgat
     1861 ccacagacac ttgagattct tgacattaca ccatgttctt ttggtggtgt cagtgttata
     1921 acaccaggaa caaatacttc taaccaggtt gctgttcttt atcaggatgt taactgcaca
     1981 gaagtccctg ttgctattca tgcagatcaa cttactccta cttggcgtgt ttattctaca
     2041 ggttctaatg tttttcaaac acgtgcaggc tgtttaatag gggctgaaca tgtcaacaac
     2101 tcatatgagt gtgacatacc cattggtgca ggtatatgcg ctagttatca gactcagact
     2161 aattctcctc ggcgggcacg tagtgtagct agtcaatcca tcattgccta cactatgtca
     2221 cttggtgcag aaaattcagt tgcttactct aataactcta ttgccatacc cacaaatttt
     2281 actattagtg ttaccacaga aattctacca gtgtctatga ccaagacatc agtagattgt
     2341 acaatgtaca tttgtggtga ttcaactgaa tgcagcaatc ttttgttgca atatggcagt
     2401 ttttgtacac aattaaaccg tgctttaact ggaatagctg ttgaacaaga caaaaacacc
     2461 caagaagttt ttgcacaagt caaacaaatt tacaaaacac caccaattaa agattttggt
     2521 ggttttaatt tttcacaaat attaccagat ccatcaaaac caagcaagag gtcatttatt
     2581 gaagatctac ttttcaacaa agtgacactt gcagatgctg gcttcatcaa acaatatggt
     2641 gattgccttg gtgatattgc tgctagagac ctcatttgtg cacaaaagtt taacggcctt
     2701 actgttttgc cacctttgct cacagatgaa atgattgctc aatacacttc tgcactgtta
     2761 gcgggtacaa tcacttctgg ttggaccttt ggtgcaggtg ctgcattaca aataccattt
     2821 gctatgcaaa tggcttatag gtttaatggt attggagtta cacagaatgt tctctatgag
     2881 aaccaaaaat tgattgccaa ccaatttaat agtgctattg gcaaaattca agactcactt
     2941 tcttccacag caagtgcact tggaaaactt caagatgtgg tcaaccaaaa tgcacaagct
     3001 ttaaacacgc ttgttaaaca acttagctcc aattttggtg caatttcaag tgttttaaat
     3061 gatatccttt cacgtcttga caaagttgag gctgaagtgc aaattgatag gttgatcaca
     3121 ggcagacttc aaagtttgca gacatatgtg actcaacaat taattagagc tgcagaaatc
     3181 agagcttctg ctaatcttgc tgctactaaa atgtcagagt gtgtacttgg acaatcaaaa
     3241 agagttgatt tttgtggaaa gggctatcat cttatgtcct tccctcagtc agcacctcat
     3301 ggtgtagtct tcttgcatgt gacttatgtc cctgcacaag aaaagaactt cacaactgct
     3361 cctgccattt gtcatgatgg aaaagcacac tttcctcgtg aaggtgtctt tgtttcaaat
     3421 ggcacacact ggtttgtaac acaaaggaat ttttatgaac cacaaatcat tactacagac
     3481 aacacatttg tgtctggtaa ctgtgatgtt gtaataggaa ttgtcaacaa cacagtttat
     3541 gatcctttgc aacctgaatt agactcattc aaggaggagt tagataaata ttttaagaat
     3601 catacatcac cagatgttga tttaggtgac atctctggca ttaatgcttc agttgtaaac
     3661 attcaaaaag aaattgaccg cctcaatgag gttgccaaga atttaaatga atctctcatc
     3721 gatctccaag aacttggaaa gtatgagcag tatataaaat ggccatggta catttggcta
     3781 ggttttatag ctggcttgat tgccatagta atggtgacaa ttatgctttg ctgtatgacc
     3841 agttgctgta gttgtctcaa gggctgttgt tcttgtggct cctgctgcaa atttgatgaa
     3901 gacgactctg agccagtgct caaaggagtc aaattacatt acacacttaa ttaagacgcg
     3961 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     4021 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     4081 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     4141 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     4201 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     4261 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     4321 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     4381 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     4441 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     4501 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     4561 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     4621 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     4681 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     4741 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     4801 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     4861 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     4921 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     4981 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     5041 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     5101 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     5161 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     5221 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     5281 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     5341 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6301 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     6361 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     6421 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     6481 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     6541 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     6601 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     6661 ggccc
//
