LOCUS       sequence-343871-        6247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ILF3 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pAPM-miR30-ILF3-ts1
FEATURES             Location/Qualifiers
     source          1..6247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(240..260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        284..494
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        306..502
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      353..488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     415..434
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        514..893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        895..1093
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1048..1068
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1111..1291
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1338..1463
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1960..2193
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2378..2422
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2720..2837
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2974..3381
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     ncRNA           4096..4110
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     LTR             4244..4424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4495..5083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4575..4594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5254..6045,6046..6114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5877..5896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6115..6219)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      421 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
      481 tggaggccta ggcttttgca aaaagctcgc gttgacattg attattgact agttattaat
      541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      901 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      961 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     1021 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     1081 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     1141 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1201 gtgcttcaag tagtgtgtgc ccgtctgttg tgagactctg gtaactagag atccctcaga
     1261 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1321 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1381 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1441 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1501 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1561 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1621 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1681 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1741 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1801 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1861 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1921 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1981 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2041 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2101 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2161 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2221 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2281 ttggaatcac acgacttgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2341 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2401 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2461 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2521 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2581 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2641 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2701 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2761 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2821 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
     2881 actgcagccc cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     2941 gaccccacct gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa
     3001 aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg
     3061 ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag
     3121 atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc
     3181 ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga
     3241 aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg
     3301 cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc
     3361 cgacagactg agtcgcccgg gggtctagaa cgcgtgccgc catgaccgaa tacaaaccta
     3421 ccgtgaggct ggctacaaga gatgatgtcc caagggctgt gagaacactg gccgccgctt
     3481 ttgccgatta ccctgccaca cgccacactg tggacccaga tcggcatatc gagagagtga
     3541 ctgagctgca ggaactgttc ctgacccgag tgggcctgga cattgggaag gtctgggtcg
     3601 cagacgatgg agcagctgtg gctgtctgga ccacaccaga gagcgtggaa gccggagctg
     3661 tctttgcaga gatcggccct agaatggcag aactgagcgg ctccaggctg gcagcacagc
     3721 agcagatgga gggactgctg gccccacaca ggcctaagga accagcatgg ttcctggcta
     3781 ccgtgggggt ctctcctgac catcagggca aaggactggg aagtgctgtg gtcctgccag
     3841 gagtggaggc tgcagaacga gctggagtcc ctgcatttct ggagacctct gctccacgaa
     3901 acctgccctt ctatgaacgg ctgggcttta ctgtgaccgc agatgtggag gtccccgaag
     3961 gacctaggac ctggtgcatg acacgcaaac ccggcgcctg agcgatcgcc gcggccgcct
     4021 tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gaaaggtgct
     4081 ggttccaaca aaatagtgaa gccacagatg tattttgttg gaaccagcac cttgtgccta
     4141 ctgcctcgga cttcaagggg ctagaattcg gcagctgtag atcttagcca ctttttaaaa
     4201 gaaaaggggg gactggaagg gctaactgca tccggactgt actgggtctc tctggttaga
     4261 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4321 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4381 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttcatgtg
     4441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4921 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6241 acctgac
//
