LOCUS       sequence-343883-       11024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (GUSPlus::tOCS in pFP100)*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI001
FEATURES             Location/Qualifiers
     source          1..11024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(619..1248)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    2579..2603
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(2680..2694,2885..4702)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          2695..2884
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4709..4726
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4739..5446
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(5689..6399,6400..6402,6403..6405))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5689..6399,6400..6402,6403..6405))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5731..5752)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6080..6099
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6339..6360
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    7041..7065
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7586..8377
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8623..9211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9112..9131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9365..9382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9397..9537
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9463..9482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9623..9645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9881..10075
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(10141..11024,1..190))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
       61 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
      121 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
      181 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
      241 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
      301 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
      361 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
      421 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
      481 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
      541 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
      601 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
      661 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
      721 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
      781 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
      841 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
      901 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
      961 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
     1021 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
     1081 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
     1141 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
     1201 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
     1261 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
     1321 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
     1381 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
     1441 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
     1501 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
     1561 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
     1621 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
     1681 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
     1741 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
     1801 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
     1861 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
     1921 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
     1981 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
     2041 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
     2101 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
     2161 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
     2221 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
     2281 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
     2341 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
     2401 cactatagca gcggaggggt tggatcaaag tactttaaag tactttaaag tactttaaag
     2461 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
     2521 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
     2581 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa
     2641 tctgatccaa gctcaagcta agcttgcatg cccgggaaaa tggtagatct gagggtaaat
     2701 ttctagtttt tctccttcat tttcttggtt aggacccttt tctcttttta tttttttgag
     2761 ctttgatctt tctttaaact gatctatttt ttaattgatt ggttatggtg taaatattac
     2821 atagctttaa ctgataatct gattacttta tttcgtgtgt ctatgatgat gatgatagtt
     2881 acagaaccga cgaactagtc tgtacccgat caacaccgag acccgtggcg tcttcgacct
     2941 caatggcgtc tggaacttca agctggacta cgggaaagga ctggaagaga agtggtacga
     3001 aagcaagctg accgacacta ttagtatggc cgtcccaagc agttacaatg acattggcgt
     3061 gaccaaggaa atccgcaacc atatcggata tgtctggtac gaacgtgagt tcacggtgcc
     3121 ggcctatctg aaggatcagc gtatcgtgct ccgcttcggc tctgcaactc acaaagcaat
     3181 tgtctatgtc aatggtgagc tggtcgtgga gcacaagggc ggattcctgc cattcgaagc
     3241 ggaaatcaac aactcgctgc gtgatggcat gaatcgcgtc accgtcgccg tggacaacat
     3301 cctcgacgat agcaccctcc cggtggggct gtacagcgag cgccacgaag agggcctcgg
     3361 aaaagtcatt cgtaacaagc cgaacttcga cttcttcaac tatgcaggcc tgcaccgtcc
     3421 ggtgaaaatc tacacgaccc cgtttacgta cgtcgaggac atctcggttg tgaccgactt
     3481 caatggccca accgggactg tgacctatac ggtggacttt caaggcaaag ccgagaccgt
     3541 gaaagtgtcg gtcgtggatg aggaaggcaa agtggtcgca agcaccgagg gcctgagcgg
     3601 taacgtggag attccgaatg tcatcctctg ggaaccactg aacacgtatc tctaccagat
     3661 caaagtggaa ctggtgaacg acggactgac catcgatgtc tatgaagagc cgttcggcgt
     3721 gcggaccgtg gaagtcaacg acggcaagtt cctcatcaac aacaaaccgt tctacttcaa
     3781 gggctttggc aaacatgagg acactcctat caacggccgt ggctttaacg aagcgagcaa
     3841 tgtgatggat ttcaatatcc tcaaatggat cggcgccaac agcttccgga ccgcacacta
     3901 tccgtactct gaagagttga tgcgtcttgc ggatcgcgag ggtctggtcg tgatcgacga
     3961 gactccggca gttggcgtgc acctcaactt catggccacc acgggactcg gcgaaggcag
     4021 cgagcgcgtc agtacctggg agaagattcg gacgtttgag caccatcaag acgttctccg
     4081 tgaactggtg tctcgtgaca agaaccatcc aagcgtcgtg atgtggagca tcgccaacga
     4141 ggcggcgact gaggaagagg gcgcgtacga gtacttcaag ccgttggtgg agctgaccaa
     4201 ggaactcgac ccacagaagc gtccggtcac gatcgtgctg tttgtgatgg ctaccccgga
     4261 gacggacaaa gtcgccgaac tgattgacgt catcgcgctc aatcgctata acggatggta
     4321 cttcgatggc ggtgatctcg aagcggccaa agtccatctc cgccaggaat ttcacgcgtg
     4381 gaacaagcgt tgcccaggaa agccgatcat gatcactgag tacggcgcag acaccgttgc
     4441 gggctttcac gacattgatc cagtgatgtt caccgaggaa tatcaagtcg agtactacca
     4501 ggcgaaccac gtcgtgttcg atgagtttga gaacttcgtg ggtgagcaag cgtggaactt
     4561 cgcggacttc gcgacctctc agggcgtgat gcgcgtccaa ggaaacaaga agggcgtgtt
     4621 cactcgtgac cgcaagccga agctcgccgc gcacgtcttt cgcgagcgct ggaccaacat
     4681 tccagatttc ggctacaaga acgctagcca tcaccatcac catcacgtgt gaaggatcct
     4741 gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt
     4801 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
     4861 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
     4921 tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct
     4981 gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat
     5041 tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac
     5101 ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg
     5161 gcgaactaat aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga
     5221 ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc
     5281 ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt
     5341 ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg
     5401 ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc tctagagcgc
     5461 cactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
     5521 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa
     5581 ttcccttatc tgggaactac tcacacatta ttatagagag agatagattt gtagagagag
     5641 actggtgatt tcagcgatgc agccgggcgg cgcttacagc tcgtccttct tgtacagctc
     5701 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     5761 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
     5821 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     5881 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     5941 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     6001 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     6061 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     6121 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     6181 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
     6241 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     6301 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     6361 gatgggcacc accccggtga acagctcctc gcccttgctc accatgtcgg ccgaggataa
     6421 tgataggaga agtgaaaaga tgagaaagag aaaaagatta gtcttcacca tggctatcgt
     6481 tcgtaaatgg tgaaaatttt cagaaaattg cttttgcttt aaaagaaatg atttaaattg
     6541 ctgcaataga agtagaatgc ttgattgctt gagattcgtt tgttttgtat atgttgtgtt
     6601 gagaattcgc ccttgttttg ctatttgtgt atgttttctt gttttggttt gagtgaagag
     6661 tgtagtgaag ggttggtttg tatttatagg tgagaaagga gatttgcatg gcaatcacgt
     6721 gtaagaatgc atgcatgcat gagtgtgtgt ggagagagag agatgctctt acgttgttgt
     6781 cgggattatg tgttggaaaa gagtgacaag tgacgaacaa aacaatacca ctcatcatag
     6841 ctccgcttat gcatgctatg tgtgatttgt agctcttgtt ctgacatgac actatgaaca
     6901 attttttaaa aagaaggtta aacttttatt taaataaaaa ccctatgtta atttggtttc
     6961 aagggcgaat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     7021 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     7081 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     7141 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     7201 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     7261 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     7321 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     7381 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     7441 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     7501 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     7561 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     7621 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     7681 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     7741 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     7801 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     7861 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     7921 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     7981 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     8041 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     8101 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     8161 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     8221 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     8281 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     8341 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     8401 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     8461 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     8521 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     8581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9361 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     9421 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     9481 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     9541 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     9601 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     9661 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     9721 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     9781 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     9841 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     9901 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     9961 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
    10021 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
    10081 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
    10141 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
    10201 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
    10261 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
    10321 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
    10381 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
    10441 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
    10501 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
    10561 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
    10621 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
    10681 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
    10741 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
    10801 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
    10861 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
    10921 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
    10981 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcg
//
