LOCUS       sequence-343884-       15583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (pGH3.17::SMT2-FLAG::tOCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 15583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI048
FEATURES             Location/Qualifiers
     source          1..15583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5239..5262
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      5271..5978
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(6221..6931,6932..6934,6935..6937))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6221..6931,6932..6934,6935..6937))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6263..6284)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6612..6631
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6871..6892
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    7573..7597
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8118..8909
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9155..9743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9644..9663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9897..9914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9929..10069
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9995..10014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10155..10177
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10413..10607
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10673..11746)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12175..12804)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    14135..14159
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtggtacacc agtcatttac ttgcctactc aatagaagtt ttcacttttg attttgtatt
       61 tctctccata agtataatat taggaataat taaagctata taagtaaaga atgcgaaaat
      121 cttgatggat ttaacattat atatatatat atatatatat atctttctag atttagattt
      181 aaactcggat tgttatgcaa tgacgcaact agtataaatg actaggctac atcttacatg
      241 ttatggtcag ctaaaaattg ttatgttaaa tgtgtgatta aaattaattt aattgaggtg
      301 tatgtacaca cgaaggattt tatattgcta tagatagata aataaataaa aataatttat
      361 actgtttgga ctgaccaagt tataacacta agaaaaacaa taatatggga attccacagt
      421 ttatttttca ctaattttca aaatccgtta ttttttttta aaaaatgttc taactaaacg
      481 agagaattaa agttatattc tcttcaagga agctggcaaa tttatttaaa gtctcacttg
      541 tacatcaaac gtacgttgaa tcatttataa gagctgagtg tgtaagatac gtaatttaaa
      601 tttgacaagt cattagcttt gccaaatatt tcgaacacta tgaagtctgt gatcattaac
      661 ttatttttct tgtcaacaga agtcacatgt tacatcacaa atcctaatta gtagaacact
      721 agaaatcaga tttagctata accatccaac atataagcta tacacttgat ttttggagac
      781 ttgtgggtaa actaactact cttgaaatgt gagactcaca tgtaagtctt gtttcggtaa
      841 ttaatctcta aattttaatc ttatgtaacc ctacgttaat agaccgaata atatttttta
      901 acttgagtaa atcgaaatca atactcatat tactagatta taatctcata tcatattcct
      961 aatttttatt gtattttttc tgtttctctt attgccattt atctgaaatt ttctttttaa
     1021 tttattttct cttatggttt tgtcgtgatt caggagtagt aattaattca cgtcatgcgc
     1081 gatttaatat gaatttaaaa atgttatctc agtccatgag aagtagtaat tgtgttgtgc
     1141 gtattggttt gaagtagtcc accaaaagag caataaatgc aaagctcgta actttgacct
     1201 attttttgtt tgtcttatta cactactgat ttttttttcc tttgccatgt ctatacagta
     1261 tacactatta ttctctctta tttttcaata tatgaactac ataacacatt taatataaac
     1321 aacatgcatt agtttacatt agatagaaaa cttaagctgt tacatgatcc aggactttgg
     1381 tatcctagtg gcatgtccgc atgtgttgca atctcttaaa actctcaaac agcaactaca
     1441 acatattcta catagtctct agatttgttt gggtcaacca tatagtctcg aatttaaacg
     1501 tcaaattcac agtacgtata tggttcttaa ccactgtatg ccaattatta tcactaaccc
     1561 tttcaattat actgtatttg aaacccgaat cttatattaa ccttagcgtt ttaggcttaa
     1621 gtttattgtt gactttcgaa ctgttaacat acttttatgg gtgtatatat atgacttatg
     1681 ctacaaccaa cttttgatcc tttaacttct agcataagtc catgactgta gtttccaatg
     1741 gatatgctta gaatcccgtt gacatatatt ttgagtgaag aattagatga tgaacaatat
     1801 aaaagtattt aagcctttta acaaaaatat aaaaatatat taaaaaaaaa tcctgttaat
     1861 aaattctttt acgggaatga ccaatcaaaa tatatgtggg cttattgttc cacgaaccag
     1921 gatgaaacac atcagatgat acatcattca aaggttttgc gaaagttctt tttatctaac
     1981 taggatttcg gatggtttgc ctaaaataac ataatagaag aaaatatggg cgttacgtat
     2041 caggaaaata ttatcacaaa acaacatttc aagaattcat tacttttaaa acagaaatag
     2101 taaggttaga aaatattcaa aaagagtgca tgtcttttaa agttgatggg tcggtcttat
     2161 agattgcgca tgtcctgtat ctctcccaaa cattgtggac cggtctatgt atttgtgaaa
     2221 ggaaatatgc caacttttac tagtctctct ctcgcatgtg tgtgagtgaa atagttttaa
     2281 tatttcaaat ttatttccaa aacaattctt acaaggatta tcaactgcgc agcttaaaaa
     2341 atccaattat actcaactag tattatcact ctatagttca gaatactttt cttgaaaata
     2401 tgaaatatat ataaactata aattttatgt ttttatatta tttaatgaag acaatcaatg
     2461 ggattaagac cattaacgtg tgaaacataa gacatatatg atttatcgta aggttgagaa
     2521 tctatgtatg ctttttcagg gaccaaaagg aatatataac atcccttcag aaaaaaaaag
     2581 atttacatga tttgggttgt tacgtttcaa aaagaaaatt aaaactctag tttaaaatct
     2641 ggtagatgtt tcagcccctt gtttaataaa tctgaaacga gattctcacc atcatttttt
     2701 tgggataaat tatcactatt attttcatat atatgttatt ttaaattgat tattaattat
     2761 ttagtagaat ggtataatca tgaattataa acaaatacgt ggaataaaat aactaaatcg
     2821 agatgtttaa ttaaatcaac ttaattgtca aggaaaagat ttattttagt tttatcatta
     2881 aatgactaaa ccaaaataaa ctaagcaata taaaaagcct aaagcaaagg aattgacgaa
     2941 acatgcctaa agtgtatgac atagcgtatt tgaatgaaat ttgttcacga gaaaaagatc
     3001 atcttctcca aaccctctca ggcctctttt ctctctgttc acatctgttt cctcgtcaca
     3061 caagacatat acattaacct aattcaactg ttccatgtct tttaaagttt tcttgatgga
     3121 attaagctga tgcatgttat tttatgttgt aattatcatc atttatggtt agttagtcaa
     3181 ttggtctatt tatattatat gtatatcaac gtaacgaccc tatcatatgc atttggatct
     3241 gcatcaccaa gatgatgact ttataatatc caagagattt aacaatctta acaatccttc
     3301 tctctcttaa tcttgattgc cataagtcaa acttgagatt atttaattac tatatggagc
     3361 ccaattatcg catgtaaatg aggttaatat aaataggcag atttaaacta acatatatgt
     3421 caatggaatt tgaataacta agagttgttt agtatttcac agcaatgcaa atatacaggt
     3481 tttaagaaca atatgcaaat gaatttaagg gatatataaa aagttcttaa agaaaattcc
     3541 atagccacat atataaagaa tttgttaact taagctatca tatacttctt tttagtataa
     3601 actttatttg aaaaatttgc atgtgagttc ataaaggttt caacgagttc ctaaataaaa
     3661 tctagctaag aggcaatatt agatgagtat agtgaagacc aagtgagtat atcttttggc
     3721 agacatatgc aaatcttgtg aatatgattg gattgatatt attcaacgga tcataaagaa
     3781 agagcttgat tggaggatta aacagcatat aaacaaatat gatattaaac aaaaaataac
     3841 atataaaatt atagggaaag cctgcacagg taatattgac caacaatagc ttctttctaa
     3901 ctagacaagg ccatgtgtgt tgtaggacct cccaaatata tattttctaa taattatttc
     3961 atcatttgcc aatttattat ataaccattt ctcatccttc tcctcatacc accaaaaacc
     4021 aaccttcaag aacagagaag aagtgattct ctaaacaaca aaaataaaat aaaactcttc
     4081 tttctctatt tctgttgaag gtacctcatc attgcaacac aaacttaaag ctttgtttgt
     4141 gtctgctttc agaaaatgga ctctttaaca ctcttcttca ccggtgcact cgtcgccgtc
     4201 ggtatctact ggttcctctg cgttctcggt ccagcagagc gtaaaggcaa acgagccgta
     4261 gatctctctg gtggctcaat ctccgccgag aaagtccaag acaactacaa acagtactgg
     4321 tctttcttcc gccgtccaaa agaaatcgaa accgccgaga aagttccaga cttcgtcgac
     4381 acattctaca atctcgtcac cgacatatac gagtggggat ggggacaatc cttccacttc
     4441 tcaccatcaa tccccggaaa atctcacaaa gacgccacgc gcctccacga agagatggcc
     4501 gtagatctga tccaagtcaa acctggtcaa aagatcctag acgtcggatg cggtgtcggc
     4561 ggtccgatgc gagcgattgc atctcactcg cgagctaacg tagtcgggat tacaataaac
     4621 gagtatcagg tgaacagagc tcgtctccac aataagaaag ctggtctcga cgcgctttgc
     4681 gaggtcgtgt gtggtaactt cctccagatg ccgttcgatg acaacagttt cgacggtgct
     4741 tattccatcg aagccacgtg tcacgcgccg aagctggaag aagtgtacgc agagatctac
     4801 agggtgttga aacccggatc tatgtatgtg tcgtacgagt gggttacgac ggagaaattt
     4861 aaggcggagg atgacgaaca cgtggaggta atccaaggga ttgagagagg cgatgcgtta
     4921 ccagggctta gggcttacgt ggatatagct gagacggcta aaaaggttgg gtttgagata
     4981 gtgaaggaga aggatctggc gagtccaccg gctgagccgt ggtggactag gcttaagatg
     5041 ggtaggcttg cttattggag gaatcacatt gtggttcaga ttttgtcagc ggttggagtt
     5101 gctcctaaag gaactgttga tgttcatgag atgttgttta agactgctga ttatttgacc
     5161 agaggaggtg aaaccggaat attctctccg atgcatatga ttctctgcag aaaaccggag
     5221 tcaccggagg agagttctga ttacaaggac gacgatgaca agtgaggatc ctgctttaat
     5281 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
     5341 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
     5401 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
     5461 gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt
     5521 ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa
     5581 atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct
     5641 tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta
     5701 ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
     5761 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
     5821 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
     5881 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
     5941 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctctagagc gccactggat
     6001 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     6061 taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct aattccctta
     6121 tctgggaact actcacacat tattatagag agagatagat ttgtagagag agactggtga
     6181 tttcagcgat gcagccgggc ggcgcttaca gctcgtcctt cttgtacagc tcgtccatgc
     6241 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     6301 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     6361 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     6421 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     6481 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     6541 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     6601 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     6661 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     6721 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     6781 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     6841 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     6901 ccaccccggt gaacagctcc tcgcccttgc tcaccatgtc ggccgaggat aatgatagga
     6961 gaagtgaaaa gatgagaaag agaaaaagat tagtcttcac catggctatc gttcgtaaat
     7021 ggtgaaaatt ttcagaaaat tgcttttgct ttaaaagaaa tgatttaaat tgctgcaata
     7081 gaagtagaat gcttgattgc ttgagattcg tttgttttgt atatgttgtg ttgagaattc
     7141 gcccttgttt tgctatttgt gtatgttttc ttgttttggt ttgagtgaag agtgtagtga
     7201 agggttggtt tgtatttata ggtgagaaag gagatttgca tggcaatcac gtgtaagaat
     7261 gcatgcatgc atgagtgtgt gtggagagag agagatgctc ttacgttgtt gtcgggatta
     7321 tgtgttggaa aagagtgaca agtgacgaac aaaacaatac cactcatcat agctccgctt
     7381 atgcatgcta tgtgtgattt gtagctcttg ttctgacatg acactatgaa caatttttta
     7441 aaaagaaggt taaactttta tttaaataaa aaccctatgt taatttggtt tcaagggcga
     7501 attcaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     7561 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     7621 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     7681 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     7741 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     7801 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
     7861 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     7921 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     7981 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     8041 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     8101 aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag
     8161 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     8221 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     8281 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     8341 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     8401 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     8461 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     8521 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     8581 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     8641 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     8701 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     8761 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     8821 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     8881 gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc
     8941 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
     9001 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     9061 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9301 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9841 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9901 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     9961 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10021 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    10081 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10141 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10201 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
    10261 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
    10321 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
    10381 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
    10441 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
    10501 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
    10561 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
    10621 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
    10681 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
    10741 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
    10801 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
    10861 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
    10921 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
    10981 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
    11041 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
    11101 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
    11161 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
    11221 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
    11281 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
    11341 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
    11401 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
    11461 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
    11521 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
    11581 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
    11641 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
    11701 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
    11761 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
    11821 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
    11881 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
    11941 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
    12001 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
    12061 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
    12121 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
    12181 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
    12241 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
    12301 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
    12361 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
    12421 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    12481 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    12541 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    12601 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    12661 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    12721 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    12781 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    12841 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    12901 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    12961 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    13021 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    13081 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    13141 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    13201 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    13261 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    13321 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    13381 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    13441 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    13501 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    13561 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    13621 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    13681 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    13741 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    13801 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    13861 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    13921 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    13981 tcaaagtact ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct
    14041 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
    14101 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
    14161 acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct
    14221 cataaacatt tacctttcat ggggattaca tttttaagtc tatagctata tccattattc
    14281 agttatgtgc ataaatcagc ttcttcttct tcttcttttg taactaaagc atgcataata
    14341 agaaagccga tattggacat agaccaacaa agctatgtaa ttggaatgat cagccaatta
    14401 ggtaacacat caaagaaaag aaataataga tcctccgatg tttggtaaat ttattaataa
    14461 ttatttacac aagttggtaa ttgtggaata atttgtctac acaaggttga caattgtggc
    14521 tgagcgatta tgttagagtg catcaagttg aaagtgtagg attttgttgg actcttttgg
    14581 tgccttggta aaataagatg atataatcgg ggtggttttg attgagcttc caccaagata
    14641 gagtgttcat attttattag ctagtagact taaagattta tgtcactcat cgtaatgaga
    14701 aatttctaca atttccactt atatacaatg caaaacaaac taaattggta caacaaacat
    14761 atattcatgt gaacctcata tgttcctcat gcatagttac caatagttcg tatctctcgt
    14821 cttttattgg gagttatctt atcaatgtgc tgaatttggc tataatgatg tttttcaaat
    14881 tgagcacacg accatgagta ctttattttt tgttttttca aaatgactta aaatcgttgt
    14941 gtatcattta tcgagtctct attcgaaagg ccagactaac tcgatttgga ctggtgccaa
    15001 gacaaaagct tgtttttggg gaggtgcgga aaggtgaaac cctccgtagt acgtgatgta
    15061 tctcaggaat agaaatgttc tgattttatc atttaatctt tgtggagttt gatgaatata
    15121 taaaggtgat ttagagtttt tatgtcatta tttacatgat tactaaaaga aaccctaggc
    15181 taaaagatat ttgcctcaaa attgaaaaag ttgaatattt taacacttta ctaatatata
    15241 ctttgttaag caccattttt ccctcctatt ttctctcctt atttcaagac tacatgtgcc
    15301 ctcatagaga cacttgcttt tgcatttcac tttatgttaa tctgagcaat aaaagacctc
    15361 taaagaccat atctaagcaa atccctgaac aaatgtccta atcaacaatt taattaatac
    15421 tagtttcttt tcggtttcaa ggcgtttacc aaaaaccaaa caactcttta cacaaaacta
    15481 gttctattca ctttgggatc ggtgatgtgc ttattgctat taattaatac tagatatgat
    15541 catagtctct tctattccaa gcaacgcata ccactttgtt tat
//
