LOCUS       sequence-343939-        6261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of MS2 Virus-Like Particles packaged with the sequence 
            for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  TITLE     A library of SARS-CoV-2 Spike gene variant virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MS2_SC2_S_Alpha
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1088..1474
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1517..1534
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1913..1960
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2006..2024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        3678..3696
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      3711..3758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3856..3873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3966..4551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4046..4065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4725..5519)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5520..5624)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5925..5947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5939..5956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5940..5956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5961..5979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5980..6004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(6013..6018,6019..6036,6037..6042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             6062..6084
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggtcgttt taactcgact ggggccaaaa cgaaacagtg gcactacccc tctccgtatt
       61 cacggggggc gttaagtgtc acatcgatag atcaaggtgc ctacaagcga agtgggtcat
      121 cgtggggtcg cccgtacgag gagaaagccg gtttcggctt ctccctcgac gcacgctcct
      181 gctacagcct cttccctgta agccaaaact tgacttacat cgaagtgccg cagaacgttg
      241 cgaaccgggc gtcgaccgaa gtcctgcaaa aggtcaccca gggtaatttt aaccttggtg
      301 ttgctttagc agaggccagg tcgacagcct cacaactcgc gacgcaaacc attgcgctcg
      361 tgaaggcgta cactgccgct cgtcgcggta attggcgcca ggcgctccgc taccttgccc
      421 taaacgaaga tcgaaagttt cgatcaaaac acgtggccgg cagatggttg gagttgcagt
      481 tcggttggtt accactaatg agtgatatcc agggtgcata tgagatgctt acgaaggttc
      541 accttcaaga gtttcttcct atgagagccg tacgtcaggt cggtactaac atcaagttag
      601 atggccgtct gtcgtatcca gctgcaaact tccagacaac gtgcaacata tcgcgacgta
      661 tcgtgatatg gttttacata aacgatgcac gtttggcatg gttgtcgtcc ctaggtatct
      721 tgaacccact aggtatagtg tgggaaaagg tgcctttctc attcgttgtc gactggctcc
      781 tacctgtagg taacatgctc gagggcctta cggcccccgt gggatgctcc tacatgtcag
      841 gaacagttac tgacgtaata acgggtgagt ccatcataag cgttgacgct ccctacgggt
      901 ggactgtgga gagacagggc actgctaagg cccaaatctc agccatgcat cgaggggtac
      961 aatccgtatg gccaacaact ggcgcgtacg taaagtctcc tttctcgatg gtccatacct
     1021 tagatgcgtt agcattaatc aggcaacggc tctctagata gagccctcaa ccggagtttg
     1081 aagcatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
     1141 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
     1201 ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
     1261 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg tagagcttcc
     1321 tgtagccgca tggcgttcgt acttaaatat ggaactaacc attccaattt tcgctacgaa
     1381 ttccgactgc gagcttattg ttaaggcaat gcaaggtctt ctaaaagatg gaaacccgat
     1441 tccctcagca atcgcagcaa actccggcat ctacgctaac tttactcagt tcgttctcgt
     1501 cgacaatggc ggtacccatc accatcacca tcacggtacc ggcgacgtga ctgtcgcccc
     1561 aagcaacttc gctaacgggg tcgctgaatg gatcagctct aactcgcgtt cacaggctta
     1621 caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt
     1681 cgaggtgcct aaagtggcaa cccagactgt tggtggtgta gagcttcctg tagccgcatg
     1741 gcgttcgtac ttaaatatgg aactaaccat tccaattttc gctacgaatt ccgactgcga
     1801 gcttattgtt aaggcaatgc aaggtcttct aaaagatgga aacccgattc cctcagcaat
     1861 cgcagcaaac tccggcatct actaatagac gccggccacc gctgagcaat aactagcata
     1921 accccttggg gcctctaaac gggtcttgag gggttttttg tcgaacagaa agtaatcgta
     1981 ttgtacacgg ccgcataatc gaaattaata cgactcacta taggagagtc caaccaacag
     2041 aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa gtttttaacg
     2101 ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac tgtgttgctg
     2161 attattctgt cctatataat tccgcatcat tttccacttt taagtgttat ggagtgtctc
     2221 ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt gtaattagag
     2281 gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat tataattata
     2341 aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat cttgattcta
     2401 aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat ctcaaacctt
     2461 ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt aatggtgttg
     2521 aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact tatggtgttg
     2581 gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca ccagcaactg
     2641 tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat ttcaacttca
     2701 atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg cctttccaac
     2761 aatttggcag agacattgat gacactactg atgctgtccg tgatccacag acacttgaga
     2821 ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca ggaacaaata
     2881 cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc cctgttgcta
     2941 ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct aatgtttttc
     3001 aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat gagtgtgaca
     3061 tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct catcggcggg
     3121 cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt gcagaaaatt
     3181 cagttgctta ctctaataac tctattgcca tacccataaa ttttactatt agtgttacca
     3241 cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg tacatttgtg
     3301 gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt acacaattaa
     3361 accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa gtttttgcac
     3421 aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt aatttttcac
     3481 aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat ctacttttca
     3541 acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc cttggtgata
     3601 ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt ttgccacctt
     3661 tgctcacaga tgaaatgaca tgaggatcac ccatgtccgc tgagcaataa ctagcataac
     3721 cccttggggc ctctaaacgg gtcttgaggg gttttttgta atttcactgc ccgctttcca
     3781 gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg
     3841 ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg
     3901 ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4321 actggtaaca ggattagcag agcgaggtat gtaggcgcta cagagttctt gaagtggtgg
     4381 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttattagaa aaattcatcc
     4741 agcagacgat aaaacgcaat acgctggcta tccggtgccg caatgccata cagcaccaga
     4801 aaacgatccg cccattcgcc gcccagttct tccgcaatat cacgggtggc cagcgcaata
     4861 tcctgataac gatccgccac gcccagacgg ccgcaatcaa taaagccgct aaaacggcca
     4921 ttttccacca taatgttcgg caggcacgca tcaccatggg tcaccaccag atcttcgcca
     4981 tccggcatgc tcgctttcag acgcgcaaac agctctgccg gtgccaggcc ctgatgttct
     5041 tcatccagat catcctgatc caccaggccc gcttccatac gggtacgcgc acgttcaata
     5101 cgatgtttcg cctgatgatc aaacggacag gtcgccgggt ccagggtatg cagacgacgc
     5161 atggcatccg ccataatgct cactttttct gccggcgcca gatggctaga cagcagatcc
     5221 tgacccggca cttcgcccag cagcagccaa tcacggcccg cttcggtcac cacatccagc
     5281 accgccgcac acggaacacc ggtggtggcc agccagctca gacgcgccgc ttcatcctgc
     5341 agctcgttca gcgcaccgct cagatcggtt ttcacaaaca gcaccggacg accctgcgcg
     5401 ctcagacgaa acaccgccgc atcagagcag ccaatggtct gctgcgccca atcatagcca
     5461 aacagacgtt ccacccacgc tgccgggcta cccgcatgca ggccatcctg ttcaatcata
     5521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5641 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5701 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5761 agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc
     5821 aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5881 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5941 taaaacgacg gccagtgagg taatacgact cactataggg gaattgtgag cggataacaa
     6001 ttcccctcta gatttacact ttatgcttcc ggctcgtatg tttctagaga attcaaataa
     6061 ttttgtttaa ctttaagaag gagatttaaa ttggctatcg ctgtaggtag ccggaattcc
     6121 attcctagga ggtttgacct gtgcgagctt ttagtaccct tgatagggag aacgaaacct
     6181 tcgtcccctc cgttcgcgtt tacgcggacg gtgagactga agataactca ttctctttaa
     6241 aatatcgttc gaactggact c
//
