LOCUS       sequence-343944-        6261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of MS2 Virus-Like Particles packaged with the sequence 
            for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  TITLE     A library of SARS-CoV-2 Spike gene variant virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MS2_SC2_S_Beta
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(4..9,10..27,28..33)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             53..75
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1340..1726
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1769..1786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2165..2212
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2258..2276
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        3930..3948
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      3963..4010
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4108..4125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4218..4803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4298..4317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4977..5771)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5772..5876)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6177..6199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6191..6208
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6192..6208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6213..6231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6232..6256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agatttacac tttatgcttc cggctcgtat gtttctagag aattcaaata attttgttta
       61 actttaagaa ggagatttaa attggctatc gctgtaggta gccggaattc cattcctagg
      121 aggtttgacc tgtgcgagct tttagtaccc ttgataggga gaacgaaacc ttcgtcccct
      181 ccgttcgcgt ttacgcggac ggtgagactg aagataactc attctcttta aaatatcgtt
      241 cgaactggac tcccggtcgt tttaactcga ctggggccaa aacgaaacag tggcactacc
      301 cctctccgta ttcacggggg gcgttaagtg tcacatcgat agatcaaggt gcctacaagc
      361 gaagtgggtc atcgtggggt cgcccgtacg aggagaaagc cggtttcggc ttctccctcg
      421 acgcacgctc ctgctacagc ctcttccctg taagccaaaa cttgacttac atcgaagtgc
      481 cgcagaacgt tgcgaaccgg gcgtcgaccg aagtcctgca aaaggtcacc cagggtaatt
      541 ttaaccttgg tgttgcttta gcagaggcca ggtcgacagc ctcacaactc gcgacgcaaa
      601 ccattgcgct cgtgaaggcg tacactgccg ctcgtcgcgg taattggcgc caggcgctcc
      661 gctaccttgc cctaaacgaa gatcgaaagt ttcgatcaaa acacgtggcc ggcagatggt
      721 tggagttgca gttcggttgg ttaccactaa tgagtgatat ccagggtgca tatgagatgc
      781 ttacgaaggt tcaccttcaa gagtttcttc ctatgagagc cgtacgtcag gtcggtacta
      841 acatcaagtt agatggccgt ctgtcgtatc cagctgcaaa cttccagaca acgtgcaaca
      901 tatcgcgacg tatcgtgata tggttttaca taaacgatgc acgtttggca tggttgtcgt
      961 ccctaggtat cttgaaccca ctaggtatag tgtgggaaaa ggtgcctttc tcattcgttg
     1021 tcgactggct cctacctgta ggtaacatgc tcgagggcct tacggccccc gtgggatgct
     1081 cctacatgtc aggaacagtt actgacgtaa taacgggtga gtccatcata agcgttgacg
     1141 ctccctacgg gtggactgtg gagagacagg gcactgctaa ggcccaaatc tcagccatgc
     1201 atcgaggggt acaatccgta tggccaacaa ctggcgcgta cgtaaagtct cctttctcga
     1261 tggtccatac cttagatgcg ttagcattaa tcaggcaacg gctctctaga tagagccctc
     1321 aaccggagtt tgaagcatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg
     1381 aactggcgac gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag
     1441 ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca
     1501 gaatcgcaaa tacaccatca aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg
     1561 tgtagagctt cctgtagccg catggcgttc gtacttaaat atggaactaa ccattccaat
     1621 tttcgctacg aattccgact gcgagcttat tgttaaggca atgcaaggtc ttctaaaaga
     1681 tggaaacccg attccctcag caatcgcagc aaactccggc atctacgcta actttactca
     1741 gttcgttctc gtcgacaatg gcggtaccca tcaccatcac catcacggta ccggcgacgt
     1801 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
     1861 ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
     1921 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg tagagcttcc
     1981 tgtagccgca tggcgttcgt acttaaatat ggaactaacc attccaattt tcgctacgaa
     2041 ttccgactgc gagcttattg ttaaggcaat gcaaggtctt ctaaaagatg gaaacccgat
     2101 tccctcagca atcgcagcaa actccggcat ctactaatag acgccggcca ccgctgagca
     2161 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgtcgaacag
     2221 aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggagag
     2281 tccaaccaac agaatctatt gttagatttc ctaatattac aaacttgtgc ccttttggtg
     2341 aagtttttaa cgccaccaga tttgcatctg tttatgcttg gaacaggaag agaatcagca
     2401 actgtgttgc tgattattct gtcctatata attccgcatc attttccact tttaagtgtt
     2461 atggagtgtc tcctactaaa ttaaatgatc tctgctttac taatgtctat gcagattcat
     2521 ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg gcaaactgga aagattgctg
     2581 attataatta taaattacca gatgatttta caggctgcgt tatagcttgg aattctaaca
     2641 atcttgattc taaggttggt ggtaattata attacctgta tagattgttt aggaagtcta
     2701 atctcaaacc ttttgagaga gatatttcaa ctgaaatcta tcaggccggt agcacacctt
     2761 gtaatggtgt taaaggtttt aattgttact ttcctttaca atcatatggt ttccaaccca
     2821 cttatggtgt tggttaccaa ccatacagag tagtagtact ttcttttgaa cttctacatg
     2881 caccagcaac tgtttgtgga cctaaaaagt ctactaattt ggttaaaaac aaatgtgtca
     2941 atttcaactt caatggttta acaggcacag gtgttcttac tgagtctaac aaaaagtttc
     3001 tgcctttcca acaatttggc agagacattg ctgacactac tgatgctgtc cgtgatccac
     3061 agacacttga gattcttgac attacaccat gttcttttgg tggtgtcagt gttataacac
     3121 caggaacaaa tacttctaac caggttgctg ttctttatca ggatgttaac tgcacagaag
     3181 tccctgttgc tattcatgca gatcaactta ctcctacttg gcgtgtttat tctacaggtt
     3241 ctaatgtttt tcaaacacgt gcaggctgtt taataggggc tgaacatgtc aacaactcat
     3301 atgagtgtga catacccatt ggtgcaggta tatgcgctag ttatcagact cagactaatt
     3361 ctcctcggcg ggcacgtagt gtagctagtc aatccatcat tgcctacact atgtcacttg
     3421 gtgtagaaaa ttcagttgct tactctaata actctattgc catacccaca aattttacta
     3481 ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa gacatcagta gattgtacaa
     3541 tgtacatttg tggtgattca actgaatgca gcaatctttt gttgcaatat ggcagttttt
     3601 gtacacaatt aaaccgtgct ttaactggaa tagctgttga acaagacaaa aacacccaag
     3661 aagtttttgc acaagtcaaa caaatttaca aaacaccacc aattaaagat tttggtggtt
     3721 ttaatttttc acaaatatta ccagatccat caaaaccaag caagaggtca tttattgaag
     3781 atctactttt caacaaagtg acacttgcag atgctggctt catcaaacaa tatggtgatt
     3841 gccttggtga tattgctgct agagacctca tttgtgcaca aaagtttaac ggccttactg
     3901 ttttgccacc tttgctcaca gatgaaatga catgaggatc acccatgtcc gctgagcaat
     3961 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg taatttcact
     4021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc atagctgttt
     4081 ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgc tacagagttc
     4621 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttattag
     4981 aaaaattcat ccagcagacg ataaaacgca atacgctggc tatccggtgc cgcaatgcca
     5041 tacagcacca gaaaacgatc cgcccattcg ccgcccagtt cttccgcaat atcacgggtg
     5101 gccagcgcaa tatcctgata acgatccgcc acgcccagac ggccgcaatc aataaagccg
     5161 ctaaaacggc cattttccac cataatgttc ggcaggcacg catcaccatg ggtcaccacc
     5221 agatcttcgc catccggcat gctcgctttc agacgcgcaa acagctctgc cggtgccagg
     5281 ccctgatgtt cttcatccag atcatcctga tccaccaggc ccgcttccat acgggtacgc
     5341 gcacgttcaa tacgatgttt cgcctgatga tcaaacggac aggtcgccgg gtccagggta
     5401 tgcagacgac gcatggcatc cgccataatg ctcacttttt ctgccggcgc cagatggcta
     5461 gacagcagat cctgacccgg cacttcgccc agcagcagcc aatcacggcc cgcttcggtc
     5521 accacatcca gcaccgccgc acacggaaca ccggtggtgg ccagccagct cagacgcgcc
     5581 gcttcatcct gcagctcgtt cagcgcaccg ctcagatcgg ttttcacaaa cagcaccgga
     5641 cgaccctgcg cgctcagacg aaacaccgcc gcatcagagc agccaatggt ctgctgcgcc
     5701 caatcatagc caaacagacg ttccacccac gctgccgggc tacccgcatg caggccatcc
     5761 tgttcaatca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5881 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5941 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6001 aatcaaaaga atagaccgag atagggttga gtggccgcta cagggcgctc ccattcgcca
     6061 ttcaggctgc gcaactgttg ggaagggcgt ttcggtgcgg gcctcttcgc tattacgcca
     6121 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6181 gtcacgacgt tgtaaaacga cggccagtga ggtaatacga ctcactatag gggaattgtg
     6241 agcggataac aattcccctc t
//
