LOCUS       sequence-343946-        6261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of MS2 Virus-Like Particles packaged with the sequence 
            for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  TITLE     A library of SARS-CoV-2 Spike gene variant virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MS2_SC2_S_Gamma
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2..7,8..25,26..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1338..1724
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1767..1784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2163..2210
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2256..2274
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        3928..3946
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      3961..4008
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4106..4123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4216..4801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4296..4315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4975..5769)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5770..5874)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6175..6197
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6189..6206
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6190..6206
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6211..6229
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6230..6254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atttacactt tatgcttccg gctcgtatgt ttctagagaa ttcaaataat tttgtttaac
       61 tttaagaagg agatttaaat tggctatcgc tgtaggtagc cggaattcca ttcctaggag
      121 gtttgacctg tgcgagcttt tagtaccctt gatagggaga acgaaacctt cgtcccctcc
      181 gttcgcgttt acgcggacgg tgagactgaa gataactcat tctctttaaa atatcgttcg
      241 aactggactc ccggtcgttt taactcgact ggggccaaaa cgaaacagtg gcactacccc
      301 tctccgtatt cacggggggc gttaagtgtc acatcgatag atcaaggtgc ctacaagcga
      361 agtgggtcat cgtggggtcg cccgtacgag gagaaagccg gtttcggctt ctccctcgac
      421 gcacgctcct gctacagcct cttccctgta agccaaaact tgacttacat cgaagtgccg
      481 cagaacgttg cgaaccgggc gtcgaccgaa gtcctgcaaa aggtcaccca gggtaatttt
      541 aaccttggtg ttgctttagc agaggccagg tcgacagcct cacaactcgc gacgcaaacc
      601 attgcgctcg tgaaggcgta cactgccgct cgtcgcggta attggcgcca ggcgctccgc
      661 taccttgccc taaacgaaga tcgaaagttt cgatcaaaac acgtggccgg cagatggttg
      721 gagttgcagt tcggttggtt accactaatg agtgatatcc agggtgcata tgagatgctt
      781 acgaaggttc accttcaaga gtttcttcct atgagagccg tacgtcaggt cggtactaac
      841 atcaagttag atggccgtct gtcgtatcca gctgcaaact tccagacaac gtgcaacata
      901 tcgcgacgta tcgtgatatg gttttacata aacgatgcac gtttggcatg gttgtcgtcc
      961 ctaggtatct tgaacccact aggtatagtg tgggaaaagg tgcctttctc attcgttgtc
     1021 gactggctcc tacctgtagg taacatgctc gagggcctta cggcccccgt gggatgctcc
     1081 tacatgtcag gaacagttac tgacgtaata acgggtgagt ccatcataag cgttgacgct
     1141 ccctacgggt ggactgtgga gagacagggc actgctaagg cccaaatctc agccatgcat
     1201 cgaggggtac aatccgtatg gccaacaact ggcgcgtacg taaagtctcc tttctcgatg
     1261 gtccatacct tagatgcgtt agcattaatc aggcaacggc tctctagata gagccctcaa
     1321 ccggagtttg aagcatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa
     1381 ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct
     1441 ctaactcgcg ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga
     1501 atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg
     1561 tagagcttcc tgtagccgca tggcgttcgt acttaaatat ggaactaacc attccaattt
     1621 tcgctacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctt ctaaaagatg
     1681 gaaacccgat tccctcagca atcgcagcaa actccggcat ctacgctaac tttactcagt
     1741 tcgttctcgt cgacaatggc ggtacccatc accatcacca tcacggtacc ggcgacgtga
     1801 ctgtcgcccc aagcaacttc gctaacgggg tcgctgaatg gatcagctct aactcgcgtt
     1861 cacaggctta caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca
     1921 ccatcaaagt cgaggtgcct aaagtggcaa cccagactgt tggtggtgta gagcttcctg
     1981 tagccgcatg gcgttcgtac ttaaatatgg aactaaccat tccaattttc gctacgaatt
     2041 ccgactgcga gcttattgtt aaggcaatgc aaggtcttct aaaagatgga aacccgattc
     2101 cctcagcaat cgcagcaaac tccggcatct actaatagac gccggccacc gctgagcaat
     2161 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg tcgaacagaa
     2221 agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta taggagagtc
     2281 caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa
     2341 gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac
     2401 tgtgttgctg attattctgt cctatataat tccgcatcat tttccacttt taagtgttat
     2461 ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt
     2521 gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaac gattgctgat
     2581 tataattata aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat
     2641 cttgattcta aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat
     2701 ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt
     2761 aatggtgtta aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact
     2821 tatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca
     2881 ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat
     2941 ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg
     3001 cctttccaac aatttggcag agacattgct gacactactg atgctgtccg tgatccacag
     3061 acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca
     3121 ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc
     3181 cctgttgcta ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct
     3241 aatgtttttc aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat
     3301 gagtgtgaca tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct
     3361 cctcggcggg cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt
     3421 gcagaaaatt cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt
     3481 agtgttacca cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg
     3541 tacatttgtg gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt
     3601 acacaattaa accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa
     3661 gtttttgcac aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt
     3721 aatttttcac aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat
     3781 ctacttttca acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc
     3841 cttggtgata ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt
     3901 ttgccacctt tgctcacaga tgaaatgaca tgaggatcac ccatgtccgc tgagcaataa
     3961 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgta atttcactgc
     4021 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc
     4081 ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg
     4141 taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4201 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcgcta cagagttctt
     4621 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttattagaa
     4981 aaattcatcc agcagacgat aaaacgcaat acgctggcta tccggtgccg caatgccata
     5041 cagcaccaga aaacgatccg cccattcgcc gcccagttct tccgcaatat cacgggtggc
     5101 cagcgcaata tcctgataac gatccgccac gcccagacgg ccgcaatcaa taaagccgct
     5161 aaaacggcca ttttccacca taatgttcgg caggcacgca tcaccatggg tcaccaccag
     5221 atcttcgcca tccggcatgc tcgctttcag acgcgcaaac agctctgccg gtgccaggcc
     5281 ctgatgttct tcatccagat catcctgatc caccaggccc gcttccatac gggtacgcgc
     5341 acgttcaata cgatgtttcg cctgatgatc aaacggacag gtcgccgggt ccagggtatg
     5401 cagacgacgc atggcatccg ccataatgct cactttttct gccggcgcca gatggctaga
     5461 cagcagatcc tgacccggca cttcgcccag cagcagccaa tcacggcccg cttcggtcac
     5521 cacatccagc accgccgcac acggaacacc ggtggtggcc agccagctca gacgcgccgc
     5581 ttcatcctgc agctcgttca gcgcaccgct cagatcggtt ttcacaaaca gcaccggacg
     5641 accctgcgcg ctcagacgaa acaccgccgc atcagagcag ccaatggtct gctgcgccca
     5701 atcatagcca aacagacgtt ccacccacgc tgccgggcta cccgcatgca ggccatcctg
     5761 ttcaatcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5881 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     5941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     6001 tcaaaagaat agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt
     6061 caggctgcgc aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc
     6121 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6181 cacgacgttg taaaacgacg gccagtgagg taatacgact cactataggg gaattgtgag
     6241 cggataacaa ttcccctcta g
//
