LOCUS       sequence-34403-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Dow EC, Liu H, Rice AP
  TITLE     T-loop phosphorylated Cdk9 localizes to nuclear speckle domains 
            which may serve as sites of active P-TEFb function and exchange 
            between the Brd4 and 7SK/HEXIM1 regulatory complexes.
  JOURNAL   J Cell Physiol. 2010 Jul . 224(1):84-93.
  PUBMED    20201073
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol. 2010 Jul . 224(1):84-93."
COMMENT     Sequence Label: pBABE-Flag-Cdk9-D167N-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..303
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             386..409
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct
       61 ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc
      121 agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac
      181 agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac
      241 agtcctgctg accaccccca ccgccctcaa agtaaacggc atcgcagctt ggatacacgc
      301 cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc cggatccatt
      361 taaattcgaa ttcagatcta ccatggacta caaagacgat gacgacaagc ttgcggccgc
      421 gatggcaaag cagtacgact cggtggagtg ccctttttgt gatgaagttt ccaaatacga
      481 gaagctcgcc aagatcggcc aaggcacctt cggggaggtg ttcaaggcca ggcaccgcaa
      541 gaccggccag aaggtggctc tgaagaaggt gctgatggaa aacgagaagg aggggttccc
      601 cattacagcc ttgcgggaga tcaagatcct tcagcttcta aaacacgaga atgtggtcaa
      661 cttgattgag atttgtcgaa ccaaagcttc cccctataac cgctgcaagg gtagtatata
      721 cctggtgttc gacttctgcg agcatgacct tgctgggctg ttgagcaatg ttttggtcaa
      781 gttcacgctg tctgagatca agagggtgat gcagatgctg cttaacggcc tctactacat
      841 ccacagaaac aag
//
