LOCUS       sequence-34408-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Wang Y, Dow EC, Liang YY, Ramakrishnan R, Liu H, Sung TL, Lin X, 
            Rice AP
  TITLE     Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 
            T-loop.
  JOURNAL   J Biol Chem. 2008 Nov 28. 283(48):33578-84.
  PUBMED    18829461
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Nov 28. 283(48):33578-84."
COMMENT     Sequence Label: HA-Cdk9 T186A
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..168
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatgtac ccatacgacg tcccagacta cgcttaccca
      121 tacgacgtcc cagactacgc ttacccatac gacgtcccag actacgctac cggtgcaaag
      181 cagtacgact cggtggagtg ccctttttgt gatgaagttt ccaaatacga gaagctcgcc
      241 aagatcggcc aaggcacctt cggggaggtg ttcaaggcca ggcaccgcaa gaccggccag
      301 aaggtggctc tgaagaaggt gctgatggaa aacgagaagg aggggttccc cattacagcc
      361 ttgcgggaga tcaagatcct tcagcttcta aaacacgaga atgtggtcaa cttgattgag
      421 atttgtcgaa ccaaagcttc cccctataac cgctgcaagg gtagtatata cctggtgctc
      481 gacttctgcg agcatgacct tgctgggctg ttgagcaatg ttttggtcaa gttcacgctg
      541 tctgagatca agagggtgat gcagatgctg cttaacggcc tctactacat ccacagaaac
      601 aagatcctgc atagggacat gaaggctgct aatgtgctta tcactcgtga tggggtcctg
      661 aagctggcag actttgggct ggcccgggcc ttcagcctgg ccaagaacag ccagcccaac
      721 cgctacgcca accgtgtggt gacactctgg taccggcccc cggagctgtt gctcggggag
      781 cgggactacg gcccccccat tgacctgtgg ggtgctgggt gcatcatggc agagatgtgg
      841 acccgcagcc ccatcatgca gggcaacacg gagcagcacc aactcgccct catca
//
