LOCUS       sequence-34412-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Greene SB, Gunaratne PH, Hammond SM, Rosen JM
  TITLE     A putative role for microRNA-205 in mammary epithelial cell 
            progenitors.
  JOURNAL   J Cell Sci. 2010 Feb 15. 123(Pt 4):606-18.
  PUBMED    20103531
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2010 Feb 15. 123(Pt 4):606-18."
COMMENT     Sequence Label: pGL3-PTEN 3'UTR
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(73..269)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      87..222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    324..415
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(356..375)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    429..477
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(745..766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
      121 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagcgatg
      181 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
      241 gactatggtt gctgactaat tgagatgcag atcgcagatc tcgagcccgg gctagcacgc
      301 gtaagagctc ggtacctatc gatagagaaa tgttctggca cctgcacttg cactggggac
      361 agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt atgttagtac
      421 tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata ttttattgcg
      481 gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag cttgggctgg
      541 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      601 gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
      661 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      781 gactccaacg tcaaagggcg aaaaaccgtc tatcaggcga tggcccacta cgtgaaccat
      841 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatc
//
