LOCUS       sequence-344141-        6499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses the extracellular and transmembrane domain of murine CD19 
            with a C terminal fusion of the SIINFEKL epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Veatch JR, Singhi N, Srivastava S, Szeto JL, Jesernig B, Stull SM, 
            Fitzgibbon M, Sarvothama M, Yechan-Gunja S, James SE, Riddell SR
  TITLE     A therapeutic cancer vaccine delivers antigens and adjuvants to 
            lymphoid tissues using genetically modified T cells.
  JOURNAL   J Clin Invest. 2021 Aug 16;131(16):e144195. doi: 10.1172/JCI144195.
  PUBMED    34396986
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2021-08-16"; volume: "131(16):e144195. doi"; pages:
            " 10.1172/JCI144195"
COMMENT     Sequence Label: pMP71-tCD19-SIINFEKL
FEATURES             Location/Qualifiers
     source          1..6499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..35
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     27..44
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2403..2806
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3312..3902
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3927..3943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3927..3943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3940..3962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3951..3967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3975..3981,3982..3999,4000..4005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4020..4041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4158..4175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4329..4917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4409..4428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5088..5879,5880..5948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5711..5730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5949..6053)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6121..6139
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6177..6199)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6299..6318
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatta gtactctagc
       61 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
      121 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
      181 gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga
      241 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
      301 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      361 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      421 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      481 ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
      541 tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc
      601 tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc
      661 gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgt
      721 gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttgg ctaactagat
      781 ctgtatctgg cggtcccgcg gaagaactga cgagttcgta ttcccggccg cagcccctgg
      841 gagacgtccc agcggcctcg ggggcccgtt ttgtggccca ttctgtatca gttaacctac
      901 ccgagtcgga ctttttggag ctccgccact gtccgagggg tacgtggctt tgttggggga
      961 cgagagacag agacacttcc cgcccccgtc tgaatttttg ctttcggttt tacgccgaaa
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agctcgacaa agttaagtaa tagtccctct ctccaagctc
     1141 acttacaggc ggccgccatg ccatctcctc tccctgtctc cttcctcctc tttcttacct
     1201 tagtaggagg caggccccag aagtccttac tggtggaggt agaagaggga ggcaatgttg
     1261 tgctgccatg cctcccggac tcctcacctg tctcttctga gaagctggct tggtatcgag
     1321 gtaaccagtc aacacccttc ctggagctga gccccgggtc ccctggcctg ggattgcacg
     1381 tggggtccct gggcatcttg ctagtgattg tcaatgtctc agaccatatg gggggcttct
     1441 acctgtgcca gaagaggccc cctttcaagg acatctggca gcctgcctgg acagtgaacg
     1501 tggaggatag tggggagatg ttccggtgga atgcttcaga cgtcagggac ctggactgtg
     1561 acctaaggaa caggtcctct gggagccaca ggtccacttc tggttcccag ctgtatgtgt
     1621 gggctaaaga ccatcctaag gtctggggaa caaagcctgt atgtgcccct cgggggagca
     1681 gtttgaatca gagtctaatc aaccaagatc tcactgtggc acccggctcc acactttggc
     1741 tgtcctgtgg ggtaccccct gtcccagtgg ccaaaggctc catctcctgg acccatgtgc
     1801 atcctaggag acctaatgtt tcactactga gcctaagcct tgggggagag cacccggtca
     1861 gagagatgtg ggtttggggg tctcttctgc ttctgcccca agccacagct ttagatgaag
     1921 gcacctatta ttgtctccga ggaaacctga ccatcgagag gcacgtgaag gtcattgcaa
     1981 ggtcagtgtg gctctggctg ttgagaactg gtggatggat agtcccagtg gtgactttag
     2041 tatatgtcat cttctgtatg gtttctctgg tggcttttct ctattgtcaa agagccttta
     2101 tcctgagaag gaaaaggggt ggcagcggcg gtgaggtgtc aggacttgaa caacttgaat
     2161 ccattattaa cttcgagaaa ctcactgaat ggacaagctc atgagaattc gagcatctta
     2221 ccgccattta ttcccatatt tgttctgttt ttcttgattt gggtatacat ttaaatgtta
     2281 ataaaacaaa atggtggggc aatcatttac attttatggg atatgtaatt actagttcag
     2341 gtgtattgcc acaagacaaa catgttaaga aactttcccg ttatttacgc tctgttcctg
     2401 ttaatcaacc tctggattac aaaatttgtg aaagattgac tgatattctt aactatgttg
     2461 ctccttttac gctgtgtgga tatgctgctt taatgcctct gtatcatgct attgcttccc
     2521 gtacggcttt cgttttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2581 tgtggcccgt tgtccgtcaa cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca
     2641 ctggctgggg cattgccacc acctgtcaac tcctttctgg gactttcgct ttccccctcc
     2701 cgatcgccac ggcagaactc atcgccgcct gccttgcccg ctgctggaca ggggctaggt
     2761 tgctgggcac tgataattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     2821 tcgcctgtgt tgccaactgg atcctgcgcg ggacgtcctt ctgctacgtc ccttcggctc
     2881 tcaatccagc ggacctccct tcccgaggcc ttctgccggt tctgcggcct ctcccgcgtc
     2941 ttcgctttcg gcctccgacg agtcggatct ccctttgggc cgcctccccg cctgtttcgc
     3001 ctcggcgtcc ggtccgtgtt gcttggtcgt cacctgtgca gaattgcgaa ccatggattc
     3061 caccgtgaac tttgtctcct ggcatgcaaa tcgtcaactt ggcatgccaa gaataattcg
     3121 gatccaagct taggcctgct cgctttcttg ctgtcccatt tctattaaag gttcctttgt
     3181 tccctaagtc caactactaa actgggggat attatgaagg gccttgagca tctggattct
     3241 gcctagcgct aagcttaaca cgagccatag atagaataaa agattttatt tagtctccag
     3301 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     3361 ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa
     3421 cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3481 ctcagggcca agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag
     3541 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca
     3601 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     3661 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     3721 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
     3781 cgtcgcccgg gtacccgtgt tctcaataaa ccctcttgca gttgcatccg actcgtggtc
     3841 tcgctgttcc ttgggagggt ctcctctgag tgattgactg cccacctcgg gggtctttca
     3901 ttctcgagca gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4141 gggcgctctt ccgctccctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6061 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     6121 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6181 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6241 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6301 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6361 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     6421 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     6481 aggcgattaa gttgggtaa
//
