LOCUS       sequence-344146-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT
            amber suppressor tRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Elsaesser Lab
  TITLE     Elsaesser plasmids 2021
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..110)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(136..591)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(273..294)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     485..504
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     718..740
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     732..749
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     733..749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   879..941
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2732..2943
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2956..3224
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3261..3968
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3406..3424)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3674..3693
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(4020..4022,4023..4025,4026..4736)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4020..4022,4023..4025,4026..4736)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4065..4086)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4326..4345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4673..4694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4740..4766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4791..5365,5366..5368,5369..5377)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4958..4975)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5185..5204
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5390..5788
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5854..5874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5916..6037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5953..5972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6007..6026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6800..6834
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7325..7341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7325..7341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7338..7360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7349..7365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7373..7379,7380..7397,7398..7403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7418..7439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7556..7573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7727..8315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7807..7826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8486..9277,9278..9341,1..5))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9109..9128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      121 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      181 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      241 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      301 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      361 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      421 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
      481 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
      541 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
      601 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      661 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      721 agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc gttcattcac gtttttgaac
      781 ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga
      841 agatgctcga cacgctgcag aacacgcagc tagattaacc ctagaaagat aatcatattg
      901 tgacgtacgt taaagataat catgtgtaaa attgacgcat gtgttttatc ggtctgtata
      961 tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac
     1021 taataataaa ttcaacaaac aatttattta tgtttattta tttattaaaa aaaacaaaaa
     1081 ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc ccccaaagcc
     1141 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
     1201 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
     1261 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
     1321 gcagacacct ggggggatac ggggaaaagg cctccacggc caactagtga aaaaccgcac
     1381 ttgtccggaa accccgggaa tctaacccgg ctgaacggat ttagagtccg ttcgatctac
     1441 atgatcaggt ttccgaggta cccaggcggc gcacaagcta tataaacctg aaggaaatct
     1501 caactttaca cttaggtcaa gttacttatc gtactagagc ttcagcagga aatttaacta
     1561 aaatctaatt taaccagcat agcaaatatc atttattccc aaaatgctaa agtttgagat
     1621 aaacggactt gatttccggc tgttttgaca ctatccagaa tgccttgcag atgggtgggg
     1681 catgctaaat actgcaggaa aaaccgcact tgtccggaaa ccccgggaat ctaacccggc
     1741 tgaacggatt tagagtccgt tcgatctaca tgatcaggtt tccgaggtac ccaggcggcg
     1801 cacaagctat ataaacctga aggaaatctc aactttacac ttaggtcaag ttacttatcg
     1861 tactagagct tcagcaggaa atttaactaa aatctaattt aaccagcata gcaaatatca
     1921 tttattccca aaatgctaaa gtttgagata aacggacttg atttccggct gttttgacac
     1981 tatccagaat gccttgcaga tgggtggggc atgctaaata ctgcaggaaa aaccgcactt
     2041 gtccggaaac cccgggaatc taacccggct gaacggattt agagtccgtt cgatctacat
     2101 gatcaggttt ccgaggtacc caggcggcgc acaagctata taaacctgaa ggaaatctca
     2161 actttacact taggtcaagt tacttatcgt actagagctt cagcaggaaa tttaactaaa
     2221 atctaattta accagcatag caaatatcat ttattcccaa aatgctaaag tttgagataa
     2281 acggacttga tttccggctg ttttgacact atccagaatg ccttgcagat gggtggggca
     2341 tgctaaatac tgcaggaaaa accgcacttg tccggaaacc ccgggaatct aacccggctg
     2401 aacggattta gagtccgttc gatctacatg atcaggtttc cgaggtaccc aggcggcgca
     2461 caagctatat aaacctgaag gaaatctcaa ctttacactt aggtcaagtt acttatcgta
     2521 ctagagcttc agcaggaaat ttaactaaaa tctaatttaa ccagcatagc aaatatcatt
     2581 tattcccaaa atgctaaagt ttgagataaa cggacttgat ttccggctgt tttgacacta
     2641 tccagaatgc cttgcagatg ggtggggcat gctaaatact gcagaactag tgctctagaa
     2701 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     3001 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     3061 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     3121 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     3181 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     3241 cctactctag agctagcacc atggtgagca agggcgagga ggataacatg gccatcatca
     3301 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     3361 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     3421 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     3481 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     3541 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     3601 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     3661 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     3721 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     3781 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     3841 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     3901 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     3961 tgtacaagtc cggactcaga tctcgagctc aagcttcacc atggcactag caattagcca
     4021 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4081 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4141 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4201 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4261 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4321 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4381 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4441 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4501 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4561 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4621 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4681 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagatgt
     4741 acccatacga tgttccagat tacgcttaat gataaggatc cgcggccgcg cccctctccc
     4801 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4861 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     4921 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     4981 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5041 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5101 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5161 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5221 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5281 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5341 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gcgtccggaa tggccaagcc
     5401 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     5461 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     5521 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     5581 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     5641 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5701 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     5761 gccctctggt tatgtgtggg agggctgacc atgggtcgac aatcaacctc tggattacaa
     5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5881 cgctgcttta atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg
     5941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
     6061 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
     6121 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
     6181 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
     6241 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     6301 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     6361 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     6421 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     6481 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
     6541 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     6601 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     6661 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     6721 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     6781 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     6841 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     6901 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     6961 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     7021 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     7081 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     7141 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     7201 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     7261 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     7321 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7381 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7441 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8401 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8461 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8521 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8581 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8641 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8701 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8761 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8821 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8881 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8941 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9001 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9061 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9121 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9181 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9241 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9301 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat a
//
