LOCUS       sequence-34424-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of ArchT-GFP for neuronal inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, 
            Baratta MV, Winkle J, Desimone R, Boyden ES
  TITLE     A high-light sensitivity optical neural silencer: development and 
            application to optogenetic control of non-human primate cortex.
  JOURNAL   Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
  PUBMED    21811444
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Syst 
            Neurosci."; date: "2011"; volume: "5"; pages: "18. Epub 2011 Apr 13"
COMMENT     Sequence Label: pAAV-FLEX-ArchT-GFP
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(800..833)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(904..937)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctcctcgccc ttgctcactg ctactaccgg tcggggctcg ggggcctcgg tgtcgcccag
       61 gatggccctg ctcctcagca ggatgaagcc gaagcccact ttggcggtca cgtccagcac
      121 catgaacagc agggtctcga ttcccagtcc caccactcca gctccctcgg ttccgatgat
      181 ccacaggatg gggtaggcgg tccacagcac cagcaccagg gcggtcaggg tgttgaaggt
      241 gctagccacc tctggtcccc tctccttagc agcagccctc aggctggtag ccaggaagta
      301 cagcaccacg atcatgcaga tggtgctgaa cagccaccag ctgtacctag ccagtggggt
      361 gtggctcagg gcgccgatca ggccggtcac gatcatcagg gcgtccactc ccaccagggt
      421 tccgatgctc accctgtcca ccttagccag cagggccagg tcgagcagaa gcaaaggggt
      481 ggtgaacagc cagtcggcgt atctggcgta gtagatgtcc agcacctcgc cggccacggt
      541 cacctcggtc aggccgatgc cgaagaacat gctcaggtag gcggcgctgg cgatgccggg
      601 caccaggatt gtgatgctgt agtactccct ggcctccttg tcggtcacgc cccagccctt
      661 cacgatgaag tagaaggtgc cgatcagcat cagcagggtt ccgattccca gccacagggt
      721 ctctggcctt ccgtcgccca gcangtcgta tcctgcctgc agagcgatgg ggtccatggt
      781 ggcggtaccg aattctagta taacttcgta taggatactt tatacgaagt tatcattggg
      841 attcttccta ttttgatcca agcatcacca tcgaccctct agtccagatc tcaccatcga
      901 cccataactt cgtatagcat acattatacg aagttatgtc cctcgaagag gttcg
//
