LOCUS       sequence-34428-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
  TITLE     RNAi reveals doublecortin is required for radial migration in rat 
            neocortex.
  JOURNAL   Nat Neurosci. 2003 Dec . 6(12):1277-83.
  PUBMED    14625554
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2003 Dec . 6(12):1277-83."
COMMENT     Sequence Label: DCX 3UTRhp
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    56..190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(80..99)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     134..153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(288..308)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(288..306)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(327..343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(340..362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    351..367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(375..381,382..399,400..405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    420..441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(558..575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggctatagac acaatagcct tgttggtcac ttgagctttt tctagataat acgtagatcc
       61 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      181 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      241 ggaggttttt taattcttgc ggccgcggcg gtacccagct tttgttccct ttagtgaggg
      301 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      361 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
      421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      661 ggaaagaaca tgtgagcaaa agg
//
