LOCUS       sequence-34429-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
  TITLE     RNAi reveals doublecortin is required for radial migration in rat 
            neocortex.
  JOURNAL   Nat Neurosci. 2003 Dec . 6(12):1277-83.
  PUBMED    14625554
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2003 Dec . 6(12):1277-83."
COMMENT     Sequence Label: DCX 3UTRhp
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(102..122)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(102..120)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(141..157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(141..157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(154..176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(189..195,196..213,214..219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(372..389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(623..642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaattc ttgcggccgc ggcggtaccc agcttttgtt ccctttagtg agggttaatt
      121 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      181 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
      241 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      301 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      841 aactatcgtc ttgagtccaa cccggta
//
