LOCUS       sequence-34430-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRPF1 (3MO8)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             500..517
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(570..588)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      584..631
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(755..774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcgaggact ccccgctgga tgctctggac ctcgtgtggg
      121 ccaaatgccg aggctatcca tcatacccag ctctgatcat tgatccaaag atgccccgag
      181 aaggtatgtt ccaccatggg gttcccatcc ctgtgccccc actggaggtg ctgaaacttg
      241 gggagcagat gacccaggaa gcccgagagc atctctacct cgtcctcttc tttgacaaca
      301 aacgaacctg gcagtggctg cccaggacca agctggttcc tctgggtgtg aaccaggacc
      361 tagacaagga gaagatgctg gagggccgca agtccaacat ccgcaagtca gtacagatcg
      421 cctaccacag ggctctgcag caccgcagca aggtgcaagg cgagcagagc tgatgacgaa
      481 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      541 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      601 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      661 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
//
