LOCUS       sequence-34431-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LaminB1 (3MOV)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcatggcga ctgcgacccc cgtgccgccg
      121 cggatgggca gccgcgctgg cggccccacc acgccgctga gccccacgcg cctgtcgcgg
      181 ctccaggaga aggaggagct gcgcgagctc aatgaccggc tggcggtgta catcgacaag
      241 gtgcgcagcc tggagacgga gaacagcgcg ctgcagctgc aggtgacgga gcgcgaggag
      301 gtgcgcggcc gtgagctcac cggcctcaag gcgctctacg agaccgagct ggccgacgcg
      361 cgacgcgcgc tcgacgacac ggcccgcgag cgcgccaagc tgcagatcga gctgggcaag
      421 tgcaaggcgg aacacgacca gctgctcctc aactatgcta agaaggaatc tgatcttaat
      481 ggcgcccaga tcaagcttcg agaatatgaa gcagcactga attcgaaaga tgcagctctt
      541 gctactgcac ttggtgacaa aaaaagttta gagggagatt tggaggatct gaaggatcag
      601 attgcccagt tggaagcctc cttagctgca gccaaaaaac agttagcaga tgaaacttta
      661 cttaaagtag atttggagaa tcgttgtcag agccttactg aggacttgga gtttcgcaaa
      721 agcatgtatg aagaggagat taacgagacc agaaggaagc atgaaacgcg cttggtagag
      781 gtggattctg ggcgtcaaat tgagtatgag tacaagctgg cgcaagccct tcatgagatg
      841 agagagcaac atgatgccca agtgaggctg tat
//
