LOCUS       sequence-34434-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GRM7
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     87..149
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             162..179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             201..221
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgtacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
       61 tcgggcgcgg atctcggtcc gaaaccatga aattcttagt caacgttgcc cttgttttta
      121 tggtcgtata catttcttac atctatgcgg ccgctccgga acatcaccat caccatcacg
      181 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggat atgtacgccc
      241 cgcactcaat ccggatcgag ggggacgtca ccctcggggg gctgttcccc gtgcacgcca
      301 agggtcccag cggagtgccc tgcggcgaca tcaagaggga aaacgggatc cacaggctgg
      361 aagcgatgct ctacgccctg gaccagatca acagtgatcc caacctactg cccaacgtga
      421 cgctgggcgc gcggatcctg gacacttgtt ccagggacac ttacgcgctc gaacagtcgc
      481 ttactttcgt ccaggcgctc atccagaagg acacctccga cgtgcgctgc accaacggcg
      541 aaccgccggt tttcgtcaag ccggagaaag tagttggagt gattggggct tcggggagtt
      601 cggtctccat catggtagcc aacatcctga ggctcttcca gatcccccag attagttatg
      661 catcaacggc acccgagcta agtgatgacc ggcgctatga cttcttctct cgcgtggtgc
      721 cacccgattc cttccaagcc caggccatgg tagacattgt aaaggcccta ggctggaatt
      781 atgtgtctac cctcgcatcg gaaggaagtt atggagagaa aggtgtggag tccttcacgc
      841 agatttccaa agaggc
//
