LOCUS       sequence-34435-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK6 (3MSE)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcattag cccgaacgtg ctgaacaaca tgaaatcata
      121 tatgaaacac tctaatatcc gcaatattat tatcaacatt atggcgcatg aactgtccgt
      181 gattaataat catattaaat acattaacga gctgttttac aaactggaca ccaaccacaa
      241 cggtagcctg agccatcgcg aaatttatac cgtgctggca agcgtaggta ttaaaaagtg
      301 ggacattaat cgtatcctgc aggccctgga tatcaatgat cgcggaaaca ttacctatac
      361 cgaattcatg gcgggttgtt atcgttggaa aaacattgaa tccacgtttc tgaaggccgc
      421 attcaacaaa attgataaag acgaagatgg gtatatttca aaatccgata ttgttagcct
      481 ggtgcatgac aaggtgctgg ataacaatga tattgacaac tttttcctgt ccgttcatag
      541 tatcaaaaaa ggtattccac gtgaacacat tattaacaaa attagctttc aagaatttaa
      601 ggattacatg ctgtcgacct tttgatgacg aagcttgcgg ccgcactcga ggatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
//
