LOCUS       sequence-34436-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP21 (3MTN)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtt ctggtcatgt tggccttcga aacctgggaa
      121 acacgtgctt cctgaatgct gtgctgcagt gtctgagcag cactcgacct cttcgggact
      181 tctgtctgag aagggacttc cggcaagagg tgcctggagg aggccgagcc caagagctca
      241 ctgaagcctt tgcagatgtg attggtgccc tctggcaccc tgactcctgc gaagctgtga
      301 atcctactcg attccgagct gtcttccaga aatatgttcc ctccttctct ggatacagcc
      361 agcaggatgc ccaagagttc ctgaagctcc tcatggagcg gctacacctt gaaatcaacc
      421 gccgaggccg ccgggctcca ccgatacttg ccaatggtcc agttccctct ccaccccgcc
      481 gaggaggggc tctgctagaa gaacctgagt taagtgatga tgaccgagcc aacctaatgt
      541 ggaaacgtta cctggagcga gaggacagca agattgtgga cctgtttgtg ggccagttga
      601 aaagttgtct caagtgccag gcctgtgggt atcgctccac gaccttcgag gttttttgtg
      661 acctgtccct gcccatcccc aagaaaggat ttgctggggg caaggtgtct ctgcgggatt
      721 gtttcaacct tttcactaag gaagaagagc tagagtcgga gaatgcccca gtgtgtgacc
      781 gatgtcggca gaaaactcga agtaccaaaa agttgacagt acaaagattc cctcgaatcc
      841 tcgtgctcca tctgaatcga
//
