LOCUS       sequence-34437-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SUV39H1
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             309..326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(379..397)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      393..440
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(564..583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     774..795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc gaagtcgagt acctgtgcga ttacaagaag
      121 atccgcgaac aggaatatta cctggtgaaa tggcgtggat atccagactc agagagcacc
      181 tgggagccac ggcagaatct caagtgtgtg cgtatcctca agcagttcca caaggactta
      241 gaaagggagc tgctccggcg gcaccaccgg tcaaagacct gatgacgaag cttgcggccg
      301 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      361 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      421 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      781 cttgttccaa actggaacaa cactcaaccc tatctcggtc ta
//
